STAG3 (NM_001282718) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282718, the custom clone sequence may differ by one or more nucleotides ATGTCTTCCCCGTTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCTG CCAGTCTACCCTTTGATGACAGGGACTCAAACCATACCTCAGAGGGGAATGGCGACTCTTTGTTAGCTGA TGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCACCGAAA ACAACACCGGTGGCAAAACATCCAAAGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAGTCAG AGCCACCAGCCAATGATCTTTTCAATGCTGTGAAAGCCGCCAAAAGTGACATGCAGGACTCGGGGGACTA CCCTCTCATAGCTCCAGGTCCATCCTGGAAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATTG GTCTGTCAGTGCCAGTACAGCCTCCTCTATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACTG GCCTCTCAGACTCACAAGTCCGCGCCTTCCGTCACACTAGCACCCTGGCTGCTATGAAACTGATGACCTC CCTGGTAAAAGTTGCCCTCCAACTGAGTGTGCACCAAGATAACAATCAGCGTCAGTATGAGGCTGAAAGA AACAAGGGGCCAGGGCAGAGGGCACCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAGAGCTCCAAG AGCATCAAGAGGAGATTGAGGGGATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGGA TGTCCTTCCTGAGATCCGTGCTATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCACGTCT TTCCTCACCGACAGCTATTTAAAATATATTGGTTGGACTCTGCATGATAAGCACCGAGAAGTCCGCCTGA AGTGTGTGAAGGCCCTGAAAGGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGAGCTCTTCACCAG CCGCTTCAAGGACCGGATGGTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTGGAGGCTGTCAGA TTACTGATACTTATCCTTAAGAACATGGAAGGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCAG TTGTGTATGCCTCTCATCGAGGCCTGGCCTCTGCCGCAGGCGAATTTCTGTACTGGAAACTCTTCTACCC TGAGTGCGAGATAAGAATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAGGCGCCCAGAGGACTTTCTTC CAGCTTCTGCTGTCCTTCTTTGTGGAGAGCGAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGT GGGACTGTGCAGGGGCTCGGCTGAAGGACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAA CCTGGGTGATGTGCAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTCAGAGGGG CACCCGCCTGTGGGCCGGGTCACTGGGAGGAAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATG ACAGGGTGAAGTTGACTGAGCACCTCATCCCCCTGCTGCCCCAGCTCCTGGCCAAGTTCTCAGCTGATGC AGAGAAGGTCACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACCTCCACATCTACTGCACTGGGCGCTTG GAGAAGCACCTGGAGCTGTTCCTGCAGCAACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGC TTGAGGCTGGGGCGCATGCCCTCTACCTGCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTT TGCCCGCAGCCAGCTAGTAGATTTGCTGACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGTCG TCCTTCCTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAACGCCTCTCTGCCTTCTACAACA CTCATGACCTGACTCGCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGG AGAGGTTCCTCACCAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACC CACATTTCTAAATCAGATGCTTCCCAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGTGGCCTTCTGTG AACTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGCTTTTGTCTTATTAAGTGA TCTACTTCTCATCTTTAGCCCTCAGATGATTGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTTT CCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGACC TGGGCAGTGGTGATTCCCAGGAGGATCATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTAGC CGGGTTCTGCAAGCTGTTGCTTTATGGGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACACTAC AACAAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGACCGAA GTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGCTGTACACAGAACTGCTGCAGGAGCATGGGCC CCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGAGGTTTGCCTTGAGTTTT GGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGGAAGGCATCCAGTTCTCCTTGT CTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCCTCCAAATCTGGCATTCCTGGAGCTCCTTTCAGAGTT TTCCCCCCGACTCTTCCATCAGGACAAGCAGCTTTTACTGTCCTATCTAGAAAAGTGCCTGCAGCATGTC TCCCAGGCACCTGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCTGTGGAGAACA CAGCAGAGACCAGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGGCCTGCCAAGCCTAA CAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCGCCCACGCCCACCCTC ACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGGAAGAAGATGGCTCAG AGTTGGATTTTGCCCAGGGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTGGGTCCACAATATTT CCAGACTCCACACAACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGACTCAGCCTTATGGAAGAG GACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAATGAAGAACGGCAGGATACAGACATGCAAGCAA GTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAGCTGGATATTGAGGATTT CTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282718
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282718.1, NP_001269647.1
RefSeq Size	4187 bp
RefSeq ORF	3504 bp
Locus ID	10734
UniProt ID	Q9UJ98
Protein Pathways	Oocyte meiosis
Gene Summary	The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (4) differs in the 5' UTR and lacks 2 consecutive in-frame exons in the 5' coding region compared to variant 1. The resulting shorter isoform (3) lacks an in internal protein segment compared to isoform 1.

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