SMC1L2 (SMC1B) (NM_001291501) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SMC1BETA; SMC1L2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291501, the custom clone sequence may differ by one or more nucleotides ATGGCCCACCTGGAGCTGCTGCTTGTGGAAAATTTCAAGTCGTGGCGGGGCCGCCAGGTCATTGGCCCCT TCCGGAGGTTCACCTGCATCATCGGCCCCAACGGCTCTGGAAAATCTAATGTAATGGATGCACTTAGTTT TGTAATGGGAGAGAAAATAGCTAATTTAAGAGTGAAAAATATTCAAGAACTCATTCATGGAGCACATATT GGAAAACCTATTTCTTCTTCTGCAAGTGTAAAAATTATATATGTGGAGGAAAGTGGCGAAGAGAAAACAT TTGCAAGGATTATCCGAGGGGGATGCTCAGAATTTCGCTTTAATGATAATCTTGTGAGTCGTTCTGTTTA CATTGCAGAGTTGGAAAAGATAGGCATAATAGTCAAAGCACAAAATTGTTTGGTTTTTCAGGGAACTGTA GAGTCAATTTCAGTGAAGAAACCCAAAGAAAGGACCCAGTTTTTTGAGGAAATCAGCACTTCAGGAGAGC TTATAGGAGAATATGAAGAAAAGAAAAGAAAGTTACAAAAAGCCGAAGAGGATGCACAGTTTAACTTTAA TAAGAAAAAAAATATAGCGGCAGAGCGCAGACAAGCAAAATTAGAGAAGGAAGAGGCAGAACGTTACCAG AGTCTCCTTGAAGAACTGAAAATGAACAAGATACAACTGCAGCTTTTTCAACTATACCATAATGAGAAAA AGATTCATCTCCTGAACACCAAGTTAGAGCATGTGAATAGGGATTTGAGTGTCAAAAGAGAGTCTTTGTC TCATCATGAAAACATAGTTAAAGCCAGGAAAAAGGAACATGGAATGCTAACTAGACAACTACAACAAACA GAAAAAGAATTAAAATCGGTTGAAACCCTTTTAAATCAGAAGAGGCCTCAGTACATTAAAGCCAAAGAAA ACACTTCTCACCACCTTAAGAAATTAGATGTGGCTAAGAAATCAATAAAGGACAGCGAAAAACAATGTTC TAAACAGGAAGATGATATAAAAGCCCTGGAGACAGAGCTGGCTGATTTAGATGCTGCATGGAGAAGTTTT GAAAAGCAGATTGAGGAAGAAATTTTACATAAAAAGCGAGACATTGAACTGGAAGCCAGTCAGCTGGATC GTTATAAAGAACTTAAGGAACAAGTAAGAAAGAAAGTAGCTACAATGACTCAACAACTGGAAAAACTGCA GTGGGAACAGAAGACAGATGAAGAAAGACTGGCATTTGAAAAGAGGAGGCATGGAGAAGTTCAGGGAAAT CTAAAACAAATAAAAGAACAAATAGAAGATCATAAAAAACGAATAGAGAAGTTAGAGGAGTATACAAAGA CATGCATGGATTGCTTGAAAGAGAAAAAACAGCAAGAGGAAACCCTAGTGGATGAAATTGAAAAAACAAA ATCAAGAATGTCTGAAGTTAATGAAGAATTGAATCTTATTAGAAGTGAATTGCAGAATGCTGGGATTGAT ACCCATGAGGGAAAACGTCAGCAAAAGAGAGCAGAGGTTCTGGAACACCTTAAAAGACTGTACCCAGATT CTGTGTTTGGAAGACTATTTGACCTGTGTCATCCTATTCATAAGAAATACCAGCTGGCTGTTACTAAGGT TTTTGGCCGGTTCATCACTGCCATTGTTGTAGCCTCTGAAAAGGTAGCAAAAGATTGTATTCGATTTCTG AAGGAGGAAAGAGCTGAACCTGAGACATTCCTCGCTCTAGATTACCTTGATATCAAGCCAATCAATGAAA GACTAAGGGAGCTTAAAGGCTGTAAAATGGTGATTGATGTCATAAAGACTCAGTTTCCTCAGCTGAAGAA AGTGATTCAGTTTGTGTGTGGAAATGGTCTTGTTTGTGAGACTATGGAAGAAGCAAGGCATATTGCACTC AGTGGACCTGAAAGACAGAAAACAGTAGCTCTTGATGGAACATTATTTTTAAAATCTGGAGTGATCTCTG GAGGGTCAAGTGACTTAAAATACAAGGCTAGATGCTGGGATGAGAAAGAGTTAAAGAATCTAAGAGACAG ACGAAGCCAGAAAATCCAAGAGCTAAAGGGTTTAATGAAGACACTCCGCAAAGAAACAGATTTGAAACAA ATACAGACCCTGATACAGGGAACTCAAACACGACTCAAATATTCACAAAATGAACTAGAGATGATTAAGA AGAAGCACCTTGTTGCTTTTTACCAGGAACAATCTCAGTTACAAAGTGAACTACTAAATATTGAGTCTCA ATGTATTATGTTGAGTGAAGGAATCAAGGAACGACAACGAAGAATTAAAGAATTTCAAGAAAAGATAGAT AAGGTAGAAGACGATATCTTCCAACACTTCTGTGAAGAAATTGGCGTGGAAAATATTCGTGAATTTGAGA ACAAACATGTTAAACGGCAACAAGAAATTGATCAAAAAAGATTAGAATTTGAAAAACAAAAAACTCGGCT TAATGTTCAACTTGAGTATAGTCGCAGTCACCTTAAGAAGAAACTGAATAAGATCAACACATTAAAAGAA ACTATCCAGAAAGGTAGTGAAGATATTGATCACCTAAAGAAGGCTGAAGAAAACTGTCTGCAGACAGTGA ATGAACTCATGGCAAAGCAGCAGCAACTTAAGGACATACGTGTCACTCAGAACTCCAGTGCCGAGAAAGT TCAAACTCAAATTGAAGAGGAACGGAAGAAGTTTCTGGCTGTTGATAGGGAAGTGGGGAAATTGCAAAAA GAAGTTGTAAGTATTCAAACTTCTCTGGAACAGAAACGATTAGAGAAGCATAACTTGCTGCTTGATTGCA AAGTGCAAGACATTGAGATAATCCTTTTGTCGGGGTCACTGGATGACATCATTGAAGTGGAGATGGGAAC TGAAGCAGAAAGTACCCAGGCAACAATTGATATCTATGAAAAAGAAGAAGCCTTTGAAATAGACTACAGC TCTCTAAAAGAGGATTTGAAGGCTCTACAGTCTGATCAAGAAATCGAGGCCCACCTTAGGCTCTTATTGC AGCAAGTAGCATCCCAGGAAGATATCTTACTGAAAACAGCAGCCCCAAACCTACGAGCACTGGAGAACTT AAAGACTGTCAGAGACAAGTTTCAAGAGTCCACAGATGCTTTTGAGGCCAGCAGAAAGGAAGCCAGACTG TGTAGGCAAGAGTTCGAGCAAGTGAAAAAAAGGAGATACGATCTTTTCACCCAGTGTTTTGAGCATGTCT CAATCTCAATTGATCAAATCTACAAGAAGCTCTGCAGAAACAACAGCGCCCAAGTGTCAAGTTACATCAA AGAGCAAACTCAAGACCAGTTTCAGATGATAGTCATCTCCCTAAAAGAAGAGTTCTATTCCAGAGCCGAC GCGCTGATCGGCATCTATCCTGAGTACGATGACTGCATGTTCAGCCGAGTTTTGACCCTAGATCTTTCTC AGTATCCAGACACTGAAGGCCAAGAAAGCAGCAAGAGACACGGAGAGTCCCGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001291501
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291501.1, NP_001278430.1
RefSeq Size	4044 bp
RefSeq ORF	3486 bp
Locus ID	27127
UniProt ID	Q8NDV3
Protein Pathways	Cell cycle, Oocyte meiosis
Gene Summary	SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 [PubMed 11564881]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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