MAP3K9 (NM_001284230) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MEKK9; MLK1; PRKE1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284230, the custom clone sequence may differ by one or more nucleotides ATGGAGCCCTCCAGAGCGCTTCTCGGCTGCCTAGCGAGCGCCGCCGCTGCCGCCCCGCCGGGGGAGGATG GAGCAGGGGCCGGGGCCGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGGCGGCGGCGGTGGGCCCCGGGGA GCTGGGCTGCGACGCGCCGCTGCCCTACTGGACGGCCGTGTTCGAGTACGAGGCGGCGGGCGAGGACGAG CTGACCCTGCGGCTGGGCGACGTGGTGGAGGTGCTGTCCAAGGACTCGCAGGTGTCCGGCGACGAGGGCT GGTGGACCGGGCAGCTGAACCAGCGGGTGGGCATCTTCCCCAGCAACTACGTGACCCCGCGCAGCGCCTT CTCCAGCCGCTGCCAGCCCGGCGGCGAGGACCCCAGTTGCTACCCGCCCATTCAGTTGTTAGAAATTGAT TTTGCGGAGCTCACCTTGGAAGAGATTATTGGCATCGGGGGCTTTGGGAAGGTCTATCGTGCTTTCTGGA TAGGGGATGAGGTTGCTGTGAAAGCAGCTCGCCACGACCCTGATGAGGACATCAGCCAGACCATAGAGAA TGTTCGCCAAGAGGCCAAGCTCTTCGCCATGCTGAAGCACCCCAACATCATTGCCCTAAGAGGGGTATGT CTGAAGGAGCCCAACCTCTGCTTGGTCATGGAGTTTGCTCGTGGAGGACCTTTGAATAGAGTGTTATCTG GGAAAAGGATTCCCCCAGACATCCTGGTGAATTGGGCTGTGCAGATTGCCAGAGGGATGAACTACTTACA TGATGAGGCAATTGTTCCCATCATCCACCGCGACCTTAAGTCCAGCAACATATTGATCCTCCAGAAGGTG GAGAATGGAGACCTGAGCAACAAGATTCTGAAGATCACTGATTTTGGCCTGGCTCGGGAATGGCACCGAA CCACCAAGATGAGTGCGGCAGGGACGTATGCTTGGATGGCACCCGAAGTCATCCGGGCCTCCATGTTTTC CAAAGGCAGTGATGTGTGGAGCTATGGGGTGCTACTTTGGGAGTTGCTGACTGGTGAGGTGCCCTTTCGA GGCATTGATGGCTTAGCAGTCGCTTATGGAGTGGCCATGAACAAACTCGCCCTTCCTATTCCTTCTACGT GCCCAGAACCTTTTGCCAAACTCATGGAAGACTGCTGGAATCCTGATCCCCACTCACGACCATCTTTCAC GAATATCCTGGACCAGCTAACCACCATAGAGGAGTCTGGTTTCTTTGAAATGCCCAAGGACTCCTTCCAC TGCCTGCAGGACAACTGGAAACACGAGATTCAGGAGATGTTTGACCAACTCAGGGCCAAAGAAAAGGAAC TTCGCACCTGGGAGGAGGAGCTGACGCGGGCTGCACTGCAGCAGAAGAACCAGGAGGAACTGCTGCGGCG TCGGGAGCAGGAGCTGGCCGAGCGGGAGATTGACATCCTGGAACGGGAGCTCAACATCATCATCCACCAG CTGTGCCAGGAGAAGCCCCGGGTGAAGAAACGCAAGGGCAAGTTCAGGAAGAGCCGGCTGAAGCTCAAGG ATGGCAACCGCATCAGCCTCCCTTCTGATTTCCAGCACAAGTTCACGGTGCAGGCCTCCCCTACCATGGA TAAAAGGAAGAGTCTTATCAACAGCCGCTCCAGTCCTCCTGCAAGCCCCACCATCATTCCTCGCCTTCGA GCCATCCAGTTGACACCAGGTGAAAGCAGCAAAACCTGGGGCAGGAGCTCAGTCGTCCCAAAGGAGGAAG GGGAGGAGGAGGAGAAGAGGGCCCCAAAGAAGAAGGGACGGACGTGGGGGCCAGGGACGCTTGGTCAGAA GGAGCTTGCCTCGGGAGATGAAGGATCCCCTCAGAGACGTGAGAAAGCTAATGGTTTAAGTACCCCATCA GAATCTCCACATTTCCACTTGGGCCTCAAGTCCCTGGTAGATGGATATAAGCAGTGGTCGTCCAGTGCCC CCAACCTGGTGAAGGGCCCAAGGAGTAGCCCGGCCCTGCCAGGGTTCACCAGCCTTATGGAGATGGAGGA TGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACAGCATTCACCCAGCCAGTCCTACCTCTGT ATCCCATTCCCTCGTGGAGAGGATGGCGATGGCCCCTCCAGTGATGGAATCCATGAGGAGCCCACCCCAG TCAACTCGGCCACGAGTACCCCTCAGCTGACGCCAACCAACAGCCTCAAGCGGGGCGGTGCCCACCACCG CCGCTGCGAGGTGGCTCTGCTCGGCTGTGGGGCTGTTCTGGCAGCCACAGGCCTAGGGTTTGACTTGCTG GAAGCTGGCAAGTGCCAGCTGCTTCCCCTGGAGGAGCCTGAGCCACCAGCCCGGGAGGAGAAGAAAAGAC GGGAGGGTCTTTTTCAGAGGTCCAGCCGTCCTCGTCGGAGCACCAGCCCCCCATCCCGAAAGCTTTTCAA GAAGGAGGAGCCCATGCTGTTGCTAGGAGACCCCTCTGCCTCCCTGACGCTGCTCTCCCTCTCCTCCATC TCCGAGTGCAACTCCACACGCTCCCTGCTGCGCTCCGACAGCGATGAAATTGTCGTGTATGAGATGCCAG TCAGCCCAGTCGAGGCCCCTCCCCTGAGTCCATGTACCCACAACCCCCTGGTCAATGTCCGAGTAGAGCG CTTCAAACGAGATCCTAACCAATCTCTGACTCCCACCCATGTCACCCTCACCACCCCCTCGCAGCCCAGC AGTCACCGGCGGACTCCTTCTGATGGGGCCCTTAAGCCAGAGACTCTCCTAGCCAGCAGGAGCCCCTCCA GCAATGGGTTGAGCCCCAGTCCTGGAGCAGGAATGTTGAAAACCCCCAGTCCCAGCCGAGACCCAGGTGA ATTCCCCCGTCTCCCTGACCCCAATGTGGTCTTCCCCCCAACCCCAAGGCGCTGGAACACTCAGCAGGAC TCTACCTTGGAGAGACCCAAGACTCTGGAGTTTCTGCCTCGGCCGCGTCCTTCTGCCAACCGGCAACGGC TGGACCCTTGGTGGTTTGTGTCCCCCAGCCATGCCCGCAGCACCTCCCCAGCCAACAGCTCCAGCACAGA GACGCCCAGCAACCTGGACTCCTGCTTTGCTAGCAGTAGCAGCACTGTAGAGGAGCGGCCTGGACTTCCA GCCCTGCTCCCGTTCCAGGCAGGGCCGCTGCCCCCGACTGAGCGGACGCTCCTGGACCTGGATGCAGAGG GGCAGAGTCAGGACAGCACCGTGCCGCTGTGCAGAGCGGAACTGAACACACACAGGCCTGCCCCTTATGA GATCCAGCAGGAGTTCTGGTCTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001284230
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284230.1, NP_001271159.1
RefSeq Size	11169 bp
RefSeq ORF	3315 bp
Locus ID	4293
UniProt ID	P80192
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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