USP8 (NM_001283049) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HumORF8; PITA4; SPG59; UBPY
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001283049, the custom clone sequence may differ by one or more nucleotides ATGCCTGCTGTGGCTTCAGTTCCTAAAGAACTCTACCTCAGTTCTTCACTAAAAGACCTTAATAAGAAGA CAGAAGTTAAACCAGAGAAAATAAGCACTAAGAGATATGAAGAAGCTGAAGTCCGGAAAAAACTTGAGGA AAAAGACAGGCAGGAGGAAGCACAGCGGCTACAACAAAAAAGGCAGGAAACAGGAAGAGAGGATGGTGGC ACATTGGCTAAAGGCTCTTTGGAGAATGTTTTGGATTCCAAAGACAAAACCCAAAAGAGCAATGGTGAAA AGAATGAAAAATGTGAGACCAAAGAGAAAGGAGCAATCACAGCAAAGGAACTATACACAATGATGACGGA TAAAAACATCAGCTTGATTATAATGGATGCTCGAAGAATGCAGGATTATCAGGATTCCTGTATTTTACAT TCTCTCAGTGTTCCTGAAGAAGCCATCAGTCCAGGAGTCACTGCTAGTTGGATTGAAGCACACCTGCCAG ATGATTCTAAAGACACATGGAAGAAGAGGGGGAATGTGGAGTATGTGGTACTTCTTGACTGGTTTAGTTC TGCCAAAGATTTACAGATTGGAACAACTCTCCGGAGTCTGAAAGATGCACTTTTCAAGTGGGAAAGTAAA ACTGTCCTGCGCAATGAGCCTTTGGTTTTAGAGGGAGGCTATGAAAACTGGCTCCTTTGTTATCCCCAGT ATACAACAAATGCTAAGGTCACTCCACCCCCACGACGCCAGAATGAAGAGGTGTCTATCTCATTGGATTT TACTTATCCCTCATTGGAAGAATCAATTCCTTCTAAACCTGCTGCCCAGACGCCACCTGCATCTATAGAA GTAGATGAAAATATAGAATTGATAAGTGGTCAAAATGAGAGAATGGGACCACTGAATATATCAACTCCAG TTGAACCAGTTGCTGCTTCTAAATCTGATGTTTCACCCATAATTCAGCCAGTGCCTAGTATAAAGAATGT TCCACAGATTGATCGTACTAAAAAACCAGCAGTCAAATTGCCTGAAGAGCATAGAATAAAATCTGAAAGT ACAAACCATGAGCAACAATCTCCTCAGAGTGGAAAAGTTATTCCTGATCGTTCCACCAAGCCAGTAGTTT TTTCTCCAACTCTCATGTTAACAGATGAAGAAAAGGCTCGTATTCATGCAGAAACTGCTCTTCTAATGGA AAAAAACAAACAAGAAAAAGAACTTCGGGAAAGGCAGCAAGAGGAACAGAAAGAGAAACTGAGGAAGGAA GAACAAGAACAAAAAGCCAAAAAGAAACAAGAAGCTGAAGAAAATGAAATTACAGAGAAGCAACAAAAAG CAAAAGAAGAAATGGAGAAGAAAGAAAGTGAACAGGCCAAGAAAGAAGATAAAGAAACCTCAGCAAAGAG GGGCAAAGAAATAACAGGAGTAAAAAGACAAAGTAAAAGTGAACATGAAACTTCTGATGCCAAGAAATCT GTAGAAGATAGGGGGAAAAGGTGTCCAACCCCAGAAATACAGAAAAAGTCAACAGGAGATGTGCCCCATA CATCTGTGACAGGGGATTCAGGTTCAGGCAAGGCTCAACGAGAACCTTTGACAAGAGCACGAAGTGAAGA AATGGGGAGGATCGTACCAGGACTGCCTTCAGGCTGGGCCAAGTTTCTTGACCCAATCACTGGAACCTTT CGTTATTATCATTCACCCACCAACACTGTTCATATGTACCCACCGGAAATGGCTCCTTCATCTGCACCTC CTTCCACCCCTCCAACTCATAAAGCCAAGCCACAGATTCCTGCTGAGCGGGATAGGGAACCTTCCAAACT GAAGCGCTCCTACTCCTCCCCAGATATAACCCAGGCTATTCAAGAGGAAGAGAAGAGGAAGCCAACAGTA ACTCCAACAGTTAATCGGGAAAACAAGCCAACATGTTATCCTAAAGCTGAGATCTCAAGGCTTTCTGCTT CTCAGATTCGGAACCTCAATCCTGTTTTTGGAGGTTCTGGACCAGCTCTTACTGGACTTCGTAACTTAGG AAATACTTGTTATATGAACTCAATATTGCAGTGCCTATGTAACGCTCCACATTTGGCTGATTATTTCAAC CGAAACTGTTATCAGGATGATATTAACAGGTCAAATTTGTTGGGGCATAAAGGTGAAGTGGCAGAAGAAT TTGGTATAATCATGAAAGCCCTGTGGACAGGACAGTATAGATATATCAGTCCAAAGGACTTTAAAATCAC CATTGGGAAGATCAATGACCAGTTTGCAGGATACAGTCAGCAAGATTCACAAGAATTGCTTCTGTTCCTA ATGGATGGTCTCCATGAAGATCTAAATAAAGCTGATAATCGGAAGAGATATAAAGAAGAAAATAATGATC ATCTCGATGACTTTAAAGCTGCAGAACATGCCTGGCAGAAACACAAGCAGCTCAATGAGTCTATTATTGT TGCACTTTTTCAGGGTCAATTCAAATCTACAGTACAGTGCCTCACATGTCACAAAAAGTCTAGGACATTT GAGGCCTTCATGTATTTGTCTCTACCACTAGCATCCACAAGTAAATGTACATTACAGGATTGCCTTAGAT TATTTTCCAAAGAAGAAAAACTCACAGATAACAACAGATTTTACTGCAGTCATTGCAGAGCTCGACGGGA TTCTCTAAAAAAGATAGAAATCTGGAAGTTACCACCTGTGCTTTTAGTGCATCTGAAACGTTTTTCCTAC GATGGCAGGTGGAAACAAAAATTACAGACATCTGTGGACTTCCCGTTAGAAAATCTTGACTTGTCACAGT ATGTTATTGGTCCAAAGAACAATTTGAAGAAATATAATTTGTTTTCTGTTTCAAATCACTACGGTGGGCT GGATGGAGGCCACTACACAGCCTATTGTAAAAATGCAGCAAGACAACGGTGGTTTAAGTTTGATGATCAT GAAGTTTCTGATATCTCCGTTTCTTCTGTGAAATCTTCAGCAGCTTATATCCTCTTTTATACTTCATTGG GACCACGAGTAACTGATGTAGCCACATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001283049
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001283049.1, NP_001269978.1
RefSeq Size	5250 bp
RefSeq ORF	3039 bp
Locus ID	9101
UniProt ID	P40818
Protein Families	Druggable Genome, Protease
Protein Pathways	Endocytosis
Gene Summary	This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) lacks three in-frame exons in the coding region compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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