TMEM16B (ANO2) (NM_001278596) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C12orf3; TMEM16B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278596, the custom clone sequence may differ by one or more nucleotides ATGGCGACTCCCGGGCCGCGCGATATACCCCTGCTCCCTGGCTCCCCACGCCGGCTGAGCCCTCAGGCAG GGTCCAGAGGGGGCCAGGGCCCCAAACATGGACAGCAGTGTCTCAAGATGCCAGGTCCCCGGGCCCCAGG TCTGCAGGGCGGTTCCAACAGAGATCCTGGCCAGCCCTGCGGTGGAGAGAGCACCCGCAGCAGCTCTGTC ATCAACAACTATCTGGATGCCAATGAGCCTGTGTCCTTGGAGGCCCGTCTTAGCCGCATGCACTTCCATG ACAGTCAGAGGAAGGTCGACTATGTACTTGCCTACCACTACCGGAAACGCGGGGTGCACCTGGCCCAAGG CTTCCCTGGCCACTCGCTGGCTATCGTCTCCAATGGGGAGACAGGCAAGGAGCCTCATGCTGGGGGCCCA GGTGACATTGAGCTGGGACCGCTCGATGCCCTGGAGGAGGAGAGGAAGGAGCAGCGGGAGGAATTTGAGC ACAATCTGATGGAGGCTGGACTGGAGCTTGAGAAGGACTTGGAGAATAAAAGCCAGGGATCCATCTTTGT CCGGATACACGCCCCGTGGCAGGTGCTGGCCAGAGAGGCAGAATTCTTGAAGATCAAAGTTCCTACCAAG AAAGAGATGTACGAGATCAAAGCAGGAGGCAGCATTGCAAAGAAGTTCAGCGCGGCTCTGCAGAAGCTGA GCTCGCACCTGCAGCCCCGAGTTCCAGAACACAGCAACAACAAGATGAAAAACCTCTCCTACCCATTCTC CAGGGAGAAGATGTACCTGTACAACATCCAGGAAAAGGACACCTTCTTTGATAATGCCACCCGCAGCCGC ATTGTGCACGAGATCCTGAAGCGCACAGCCTGCTCCCGAGCCAACAACACGATGGGTATTAACTCTCTGA TCGCAAACAATATCTATGAGGCTGCCTACCCTCTTCATGACGGTGAATACGATAGTCCAGAGGACGATAT GAATGACAGGAAGCTGCTATATCAAGAATGGGCGCGCTATGGAGTGTTCTATAAGTTCCAACCTATTGAC CTCATCAGAAAGTATTTTGGAGAAAAAATTGGACTGTATTTTGCCTGGCTGGGATTATATACATCATTCC TCATCCCATCTTCTGTAATTGGAGTGATTGTGTTTCTTTATGGATGTGCAACAATTGAAGAAGATATTCC CAGCAGAGAGATGTGTGACCAGCAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTCCTGTGATTAC TGGAACCTCAGCTCAGCCTGTGGGACCGCGCAGGCCAGCCACCTGTTTGACAACCCTGCCACCGTCTTCT TCTCTATCTTCATGGCTCTGTGGGCTACCATGTTCCTGGAAAACTGGAAGAGGCTACAGATGCGACTGGG CTACTTTTGGGACCTGACTGGCATAGAAGAGGAAGAAGAACGTGCCCAGGAACATTCCAGGCCTGAGTAT GAAACCAAAGTTCGAGAGAAAATGCTAAAGGAGAGCAACCAGTCTGCTGTCCAGAAATTGGAAACAAACA CGACGGAGTGTGGCGATGAGGATGATGAAGATAAACTGACCTGGAAGGATCGTTTCCCAGGTTACCTGAT GAACTTTGCCTCCATCTTATTCATGATTGCCCTGACATTCTCAATCGTCTTTGGGGTGATAGTGTATCGA ATAACAACTGCAGCCGCTCTGTCTCTCAATAAGGCTACACGCTCCAATGTCCGGGTGACAGTGACAGCAA CAGCAGTCATCATCAACCTCGTGGTCATCCTCATCCTGGACGAGATCTACGGCGCTGTGGCCAAGTGGCT CACCAAAATTGAGGTTCCGAAAACAGAACAGACTTTTGAAGAGCGCCTGATCCTCAAAGCTTTCTTGCTC AAGTTTGTCAATGCCTACTCCCCCATCTTCTATGTGGCCTTTTTCAAAGGGAGGTTTGTGGGCAGGCCTG GAAGCTACGTCTATGTATTCGATGGTTACCGCATGGAAGAGTGTGCTCCAGGGGGCTGTCTCATGGAGCT CTGCATTCAGCTCAGCATCATCATGTTGGGGAAGCAGTTGATCCAGAACAACATCTTTGAGATTGGAGTC CCGAAGCTAAAGAAACTATTTCGAAAGCTGAAAGATGAGACCGAAGCTGGAGAAACTGACTCTGCCCATT CGAAACATCCAGAGCAGTGGGACCTAGACTACAGCTTGGAACCATACACAGGACTGACTCCGGAGTACAT GGAAATGATCATCCAGTTTGGTTTTGTCACCCTCTTCGTGGCCTCCTTTCCCCTGGCACCTGTGTTTGCC CTCCTCAACAACGTCATTGAAGTGCGGCTCGATGCAAAGAAGTTTGTTACAGAGCTGAGACGGCCGGATG CTGTAAGAACCAAAGATATCGGAATCTGGTTTGACATTCTCTCTGGAATTGGCAAGTTCTCTGTTATCAG CAACGCTTTTGTCATTGCGATCACCTCCGACTTTATCCCCCGCCTGGTGTACCAGTACTCCTACAGTCAC AATGGGACTCTGCACGGCTTTGTCAACCACACCCTCTCCTTTTTCAACGTCAGCCAGCTGAAGGAGGGGA CGCAGCCAGAAAACTCACAGTTTGACCAGGAGGTTCAGTTCTGCAGGTTTAAGGATTACCGAGAGCCGCC ATGGGCCCCGAACCCTTATGAGTTTTCGAAACAGTACTGGTTTATTCTGTCCGCCCGTCTGGCTTTTGTC ATAATCTTCCAGAACCTCGTGATGTTCCTGAGCGTCCTCGTGGACTGGATGATTCCAGACATCCCCACGG ACATCAGCGACCAGATCAAGAAAGAGAAGAGCTTATTAGTGGATTTCTTCCTGAAAGAGGAGCATGAGAA GCTCAAGCTGATGGATGAGCCGGCTCTGAGGAGCCCAGGAGGTGGGGATCGAAGCAGGAGCCGGGCAGCC AGCTCAGCACCTTCAGGCCAAAGCCAGCTGGGCAGCATGATGTCGTCAGGCTCTCAGCACACCAATGTGT GA
Restriction Sites	SgfI-MluI
ACCN	NM_001278596
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278596.1, NP_001265525.1
RefSeq Size	3711 bp
RefSeq ORF	3012 bp
Locus ID	57101
UniProt ID	Q9NQ90
Protein Families	Transmembrane
Gene Summary	ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011] Transcript Variant: This variant (1) encodes the longer isoform (1).

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