NLRX1 (NM_001282358) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CLR11.3; DLNB26; NOD5; NOD9; NOD26
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282358, the custom clone sequence may differ by one or more nucleotides ATGAGGTGGGGCCACCATTTGCCCAGGGCCTCTTGGGGCTCTGGTTTTAGAAGAGCACTCCAGCGACCAG ATGATCGTATCCCCTTCCTGATCCACTGGAGTTGGCCCCTTCAAGGGGAGCGTCCCTTTGGGCCCCCTAG GGCCTTTATACGCCACCACGGAAGCTCGGTAGATAGCGCTCCCCCACCCGGGAGGCATGGACGGCTGTTC CCCAGCGCCTCTGCAACTGAAGCTATACAGCGGCACCGCCGGAACCTGGCTGAGTGGTTCAGCCGGCTGC CCAGGGAGGAGCGCCAGTTTGGCCCAACCTTTGCCCTAGACACGGTCCACGTTGACCCTGTGATCCGCGA GAGTACCCCTGATGAGCTACTTCGCCCACCCGCGGAGCTGGCCCTGGAGCATCAGCCACCCCAGGCCGGG CTCCCCCCACTGGCCTTGTCTCAGCTCTTTAACCCGGATGCCTGTGGGCGCCGGGTGCAGACAGTGGTGC TGTATGGGACAGTGGGCACAGGCAAGAGCACGCTGGTGCGCAAGATGGTTCTGGACTGGTGTTATGGGCG GCTGCCGGCCTTCGAGCTGCTCATCCCCTTCTCCTGTGAGGACCTGTCATCCCTGGGCCCTGCCCCAGCC TCCCTGTGCCAACTTGTGGCCCAGCGCTACACGCCCCTGAAGGAGGTTCTGCCCCTGATGGCTGCTGCTG GGTCCCACCTCCTCTTTGTGCTCCATGGCTTAGAGCATCTCAACCTCGACTTCCGGCTGGCAGGCACGGG ACTTTGTAGTGACCCGGAGGAACCGCAGGAACCAGCTGCTATCATCGTCAACCTGCTGCGCAAATACATG CTGCCTCAGGCCAGCATTCTGGTGACCACTCGGCCCTCTGCCATTGGCCGTATCCCCAGCAAGTACGTGG GCCGCTATGGTGAGATCTGCGGTTTCTCTGATACCAACCTGCAGAAGCTCTACTTCCAGCTCCGCCTCAA CCAGCCGTACTGCGGGTATGCCGTTGGCGGTTCAGGTGTCTCTGCCACACCAGCTCAGCGTGACCACCTG GTGCAGATGCTCTCCCGGAACCTGGAGGGGCACCACCAGATAGCCGCTGCCTGCTTCCTGCCGTCCTATT GCTGGCTCGTTTGTGCCACCTTGCACTTCCTGCATGCCCCCACGCCTGCTGGGCAGACCCTTACAAGCAT CTATACCAGCTTCCTGCGCCTCAACTTCAGCGGGGAAACCCTGGACAGCACTGACCCCTCCAATTTGTCC CTGATGGCCTATGCAGCCCGAACCATGGGCAAGTTGGCCTATGAGGGGGTGTCCTCCCGCAAGACCTACT TCTCTGAAGAGGATGTCTGTGGCTGCCTGGAGGCTGGCATCAGGACGGAGGAGGAGTTTCAGCTGCTGCA CATCTTCCGTCGGGATGCCCTGAGGTTTTTCCTGGCCCCATGTGTGGAGCCAGGGCGTGCAGGCACCTTC GTGTTCACCGTGCCCGCCATGCAGGAATACCTGGCTGCCCTCTACATTGTGCTGGGTTTGCGCAAGACGA CCCTGCAAAAGGTGGGCAAGGAAGTGGCTGAGCTCGTGGGCCGTGTTGGGGAGGACGTCAGCCTGGTACT GGGCATCATGGCCAAGCTGCTGCCTCTGCGGGCTCTGCCTCTGCTCTTCAACCTGATCAAGGTGGTTCCA CGAGTGTTTGGGCGCATGGTGGGTAAAAGCCGGGAGGCGGTGGCTCAGGCCATGGTGCTGGAGATGTTTC GAGAGGAGGACTACTACAACGATGATGTTCTGGACCAGATGGGCGCCAGTATCCTGGGCGTGGAGGGCCC CCGGCGCCACCCAGATGAGCCCCCTGAGGATGAAGTCTTCGAGCTCTTCCCCATGTTCATGGGGGGGCTT CTCTCTGCCCACAACCGAGCTGTGCTAGCTCAGCTTGGCTGCCCCATCAAGAACCTGGATGCCCTGGAGA ATGCCCAGGCCATCAAGAAGAAGCTGGGCAAGCTGGGCCGGCAGGTGCTGCCCCCATCAGAGCTCCTTGA CCACCTCTTCTTCCACTATGAGTTCCAGAACCAGCGCTTCTCCGCTGAGGTGCTCAGCTCCCTGCGTCAG CTCAACCTGGCAGGTGTGCGCATGACACCAGTCAAGTGCACAGTGGTGGCAGCTGTGCTGGGCAGCGGAA GGCATGCCCTGGATGAGGTGAACTTGGCCTCCTGCCAGCTAGATCCTGCTGGGCTGCGCACACTCCTGCC TGTCTTCCTGCGTGCCCGGAAGCTGGGCTTGCAACTCAACAGCCTGGGCCCTGAGGCCTGCAAGGACCTC CGAGACCTGTTGCTGCATGACCAGTGCCAAATTACCACACTGCGGCTGTCCAACAACCCGCTGACGGCGG CAGGTGTTGCCGTGCTAATGGAGGGGCTGGCAGGAAACACCTCAGTGACGCACCTGTCCCTGCTGCACAC GGGCCTTGGGGACGAAGGCCTGGAGCTGCTGGCTGCCCAGCTGGACCGCAACCGGCAGCTGCAGGAGCTG AACGTGGCGTACAACGGTGCTGGTGACACAGCGGCCCTGGCCCTGGCCAGAGCTGCCCGGGAGCACCCTT CCCTGGAACTGCTACACCTCTACTTCAATGAGCTGAGCTCAGAGGGCCGCCAGGTCTTGCGAGACTTGGG GGGTGCTGCTGAAGGTGGTGCCCGGGTGGTGGTGTCACTGACAGAGGGGACGGCGGTGTCAGAATACTGG TCAGTGATCCTCAGTGAAGTCCAGCGGAACCTCAATAGCTGGGATCGGGCCCGGGTTCAGCGACACCTTG AGCTCCTACTGCGGGATCTGGAAGATAGCCGGGGTGCCACCCTTAATCCTTGGCGCAAGGCCCAGCTGCT GCGAGTGGAGGGCGAGGTCAGGGCCCTCCTGGAGCAGCTGGGAAGCTCTGGAAGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282358
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282358.1, NP_001269287.1
RefSeq Size	3682 bp
RefSeq ORF	2928 bp
Locus ID	79671
UniProt ID	Q86UT6
Protein Pathways	RIG-I-like receptor signaling pathway
Gene Summary	The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 4. All variants encode the same protein.

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