Vesicle docking protein p115 (USO1) (NM_001290049) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	P115; TAP; VDP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290049, the custom clone sequence may differ by one or more nucleotides ATGAATTTCCTCCGCGGGGTAATGGGGGGTCAGAGTGCCGGACCCCAGCACACAGAAGCCGAGACGATTC AAAAGCTTTGTGACAGAGTAGCTTCATCTACTTTATTGGATGATCGAAGAAATGCTGTTCGTGCTCTCAA ATCATTATCTAAGAAATACCGCTTGGAAGTGGGTATACAAGCTATGGAACATCTTATTCATGTTTTACAA ACAGATCGTTCAGATTCTGAAATAATAGGTTATGCTTTGGACACACTATATAATATAATATCTAATGAAG AAGAAGAAGAAGTAGATGATGTTGAAGAAGAAAATTCCACAAGACAGAGTGAAGATTTGGGAAGCCAATT TACAGAAATTTTCATTAAGCAGCAGGAAAATGTCACTCTTCTGTTATCTTTATTGGAGGAGTTTGATTTC CATGTCCGCTGGCCTGGTGTGAAGCTTCTTACTTCTCTTTTAAAACAACTAGGGCCTCAGGTGCAACAAA TTATTTTAGTCAGTCCTATGGGTGTTTCAAGATTGATGGACTTACTAGCGGATTCCAGGGAAGTTATACG TAATGATGGCGTCTTACTACTGCAGGCACTAACAAGAAGCAATGGTGCAATCCAGAAAATTGTTGCTTTT GAAAATGCTTTCGAGAGACTACTGGACATTATTTCAGAGGAGGGGAACAGTGATGGAGGTATAGTAGTTG AAGATTGTTTGATTTTGCTCCAAAACTTATTAAAAAACAACAACTCCAATCAAAATTTTTTTAAAGAAGG CTCATATATTCAACGTATGAAACCTTGGTTTGAAGTTGGAGATGAAAATTCTGGCTGGTCTGCACAGAAA GTGACCAATCTACATCTAATGCTACAGCTTGTTCGTGTATTGGTATCTCCCACCAACCCTCCTGGTGCTA CCAGTAGCTGCCAGAAGGCTATGTTCCAGTGTGGGTTATTGCAGCAGCTTTGTACTATCCTAATGGCTAC TGGGGTTCCTGCTGATATCCTGACTGAGACCATAAATACTGTATCAGAAGTTATTCGAGGCTGCCAAGTA AACCAAGACTACTTTGCATCTGTAAATGCACCTTCAAACCCACCAAGACCGGCAATTGTAGTACTTCTCA TGTCCATGGTTAATGAAAGGCAGCCATTTGTTTTGCGCTGTGCTGTTCTCTATTGTTTCCAGTGTTTCTT GTATAAAAACCAAAAAGGACAAGGAGAAATCGTGTCAACACTTTTACCTTCTACCATTGATGCAACAGGT AATTCAGTTTCAGCTGGCCAGTTATTATGTGGAGGTTTGTTTTCTACTGATTCACTTTCAAACTGGTGTG CTGCTGTGGCCCTTGCCCATGCGTTGCAAGAAAATGCCACCCAGAAAGAACAGTTGCTCAGGGTTCAACT TGCTACAAGTATTGGCAACCCTCCAGTTTCTTTACTTCAACAGTGCACCAATATTCTTTCACAGGGTGAT AAGATCGACAGACGGGGAAGCAAAATACAAACAAGAGTTGGATTATTAATGTTGCTTTGTACCTGGCTAA GCAATTGTCCCATTGCAGTAACGCATTTTCTTCACAATTCAGCCAATGTTCCATTTCTTACAGGACAAAT TGCAGAAAATCTTGGAGAAGAAGAGCAGTTGGTCCAAGGCTTATGTGCCCTTTTGTTGGGCATTTCGATT TATTTCAATGATAACTCACTTGAGAGCTACATGAAAGAGAAGCTAAAACAACTGATTGAGAAGAGGATTG GCAAAGAGAATTTCATAGAGAAACTAGGATTTATTAGCAAACATGAGTTGTATTCCAGAGCATCTCAGAA ACCCCAGCCAAACTTTCCCAGTCCAGAATACATGATATTTGATCATGAGTTTACGAAGCTGGTAAAAGAA CTTGAAGGTGTTATAACTAAGGCTATTTATAAGTCCAGTGAAGAAGATAAAAAAGAAGAAGAGGTGAAAA AAACATTAGAACAGCATGACAATATTGTGACTCACTACAAAAATATGATTCGAGAGCAGGATCTCCAACT TGAGGAATTAAGGCAGCAGGTTTCTACATTAAAATGTCAAAATGAACAGCTCCAGACGGCAGTCACACAG CAAGTATCACAGATCCAGCAGCACAAAGACCAGTATAATCTTCTTAAAATACAGCTAGGAAAAGACAATC AGCATCAAGGTTCTTACAGTGAGGGGGCTCAGATGAATGGCATTCAGCCAGAAGAAATTGGTAGATTGCG AGAAGAGATAGAAGAATTAAAACGTAATCAGGAACTTTTACAAAGCCAGCTGACTGAAAAGGACTCTATG ATTGAAAATATGAAATCTTCCCAAACATCTGGCACAAATGAACAGTCTTCAGCAATAGTTTCAGCTAGAG ATTCTGAACAAGTTGCAGAATTAAAACAGGAACTGGCAACTTTAAAGTCTCAGTTAAACTCACAATCTGT GGAGATCACCAAACTACAGACAGAAAAGCAGGAACTGTTACAGAAAACAGAAGCGTTTGCAAAATCAGTT GAGGTACAAGGAGAGACCGAGACTATAATAGCCACCAAAACTACTGATGTAGAAGGAAGACTGTCAGCAT TATTACAAGAGACCAAAGAGTTAAAGAATGAAATTAAAGCTCTGTCTGAGGAAAGAACTGCCATTAAAGA GCAGCTGGATTCATCTAATAGTACCATTGCCATTTTACAAACTGAGAAAGACAAACTAGAGTTGGAAATT ACAGATTCTAAAAAAGAACAAGATGATCTCTTGGTGCTCTTGGCCGATCAAGATCAGAAAATACTGTCAT TGAAGAATAAACTCAAGGATCTTGGTCATCCAGTTGAAGAAGAGGATGAACTTGAATCTGGAGACCAAGA GGATGAGGATGATGAAAGTGAAGATCCTGGCAAGGATCTAGATCATATCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001290049
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290049.1, NP_001276978.1
RefSeq Size	4222 bp
RefSeq ORF	2922 bp
Locus ID	8615
UniProt ID	O60763
Gene Summary	The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.