Hephaestin (HEPH) (NM_001282141) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CPL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282141, the custom clone sequence may differ by one or more nucleotides ATGTGGGCCATGGAGTCAGGCCACCTCCTCTGGGCTCTGCTGTTCATGCAGTCCTTGTGGCCTCAACTGA CTGATGGAGCCACTCGAGTCTACTACCTGGGCATCCGGGATGTGCAGTGGAACTATGCTCCCAAGGGAAG AAATGTCATCACGAACCAGCCTCTGGACAGTGACATAGTGGCTTCCAGCTTCTTAAAGTCTGACAAGAAC CGGATAGGGGGAACCTACAAGAAGACCATCTATAAAGAATACAAGGATGACTCATACACAGATGAAGTGG CCCAGCCTGCCTGGTTGGGCTTCCTGGGGCCAGTGTTGCAGGCTGAAGTGGGGGATGTCATTCTTATTCA CCTGAAGAATTTTGCCACTCGTCCCTATACCATCCACCCTCATGGTGTCTTCTACGAGAAGGACTCTGAA GGTTCCCTATACCCAGATGGCTCCTCTGGGCCACTGAAAGCTGATGACTCTGTTCCCCCGGGGGGCAGCC ATATCTACAACTGGACCATTCCAGAAGGCCATGCACCCACCGATGCTGACCCAGCGTGCCTCACCTGGAT CTACCATTCTCATGTAGATGCTCCACGAGACATTGCAACTGGCCTAATTGGGCCTCTCATCACCTGTAAA AGAGGAGCCCTGGATGGGAACTCCCCTCCTCAACGCCAGGATGTAGACCATGATTTCTTCCTCCTCTTCA GTGTGGTAGATGAGAACCTCAGCTGGCATCTCAATGAGAACATTGCCACTTACTGCTCAGATCCTGCTTC AGTGGACAAAGAAGATGAGACATTTCAGGAGAGCAATAGGATGCATGCAATCAATGGCTTTGTTTTTGGG AATTTACCTGAGCTGAACATGTGTGCACAGAAACGTGTGGCCTGGCACTTGTTTGGCATGGGCAATGAAA TTGATGTCCACACAGCATTTTTCCATGGACAGATGCTGACTACCCGTGGACACCACACTGATGTGGCTAA CATCTTTCCAGCCACCTTTGTGACTGCTGAGATGGTGCCCTGGGAACCTGGTACCTGGTTAATTAGCTGC CAAGTGAACAGTCACTTTCGAGATGGCATGCAGGCACTCTACAAGGTCAAGTCTTGCTCCATGGCCCCTC CTGTGGACCTGCTCACAGGCAAAGTTCGACAGTACTTCATTGAGGCCCATGAGATTCAATGGGACTATGG CCCGATGGGGCATGATGGGAGTACTGGGAAGAATTTGAGAGAGCCAGGCAGTATCTCAGATAAGTTTTTC CAGAAGAGCTCCAGCCGAATTGGGGGCACTTACTGGAAAGTGCGATATGAAGCCTTTCAAGATGAGACAT TCCAAGAGAAGATGCATTTGGAGGAAGATAGGCATCTTGGAATCCTGGGGCCAGTGATCCGGGCTGAGGT GGGTGACACCATTCAGGTGGTCTTCTACAACCGTGCCTCCCAGCCATTCAGCATGCAGCCCCATGGGGTC TTTTATGAGAAAGACTATGAAGGCACTGTGTACAATGATGGGACATTTGAGATTTATTGCCAGGCAGGCA GCCATCGAGAAGCAGGGATGAGGGCAATCTATAATGTCTCCCAGTGTCCTGGCCACCAAGCCACCCCTCG CCAACGCTACCAAGCTGCAAGAATCTACTATATCATGGCAGAAGAAGTAGAGTGGGACTATTGCCCTGAC CGGAGCTGGGAACGGGAATGGCACAACCAGTCTGAGAAGGACAGTTATGGTTACATTTTCCTGAGCAACA AGGATGGGCTCCTGGGTTCCAGATACAAGAAAGCTGTATTCAGGGAATACACTGATGGTACATTCAGGAT CCCTCGGCCAAGGACTGGACCAGAAGAACACTTGGGAATCTTGGGTCCACTTATCAAAGGTGAAGTTGGT GATATCCTGACTGTGGTATTCAAGAATAATGCCAGCCGCCCCTACTCTGTGCATGCTCATGGAGTGCTAG AATCTACTACTGTCTGGCCACTGGCTGCTGAGCCTGGTGAGGTGGTCACTTATCAGTGGAACATCCCAGA GAGGTCTGGCCCTGGGCCCAATGACTCTGCTTGTGTTTCCTGGATCTATTATTCTGCAGTGGATCCCATC AAGGACATGTATAGTGGCCTGGTGGGGCCCTTGGCTATCTGCCAAAAGGGCATCCTGGAGCCCCATGGAG GACGGAGTGACATGGATCGGGAATTTGCATTGTTGTTCTTGATTTTTGATGAAAATAAGTCTTGGTATTT GGAGGAAAATGTGGCAACCCATGGGTCCCAGGATCCAGGCAGTATTAACCTACAGGATGAAACTTTCTTG GAGAGCAATAAAATGCATGCAATCAATGGGAAACTCTATGCCAACCTTAGGGGTCTTACCATGTACCAAG GAGAACGAGTGGCCTGGTACATGCTGGCCATGGGCCAAGATGTGGATCTACACACCATCCACTTTCATGC AGAGAGCTTCCTCTATCGGAATGGCGAGAACTACCGGGCAGATGTGGTGGATCTGTTCCCAGGGACTTTT GAGGTTGTGGAGATGGTGGCCAGCAACCCTGGGACATGGCTGATGCACTGCCATGTGACTGACCATGTCC ATGCTGGCATGGAGACCCTCTTCACTGTTTTTTCTCGAACAGAACACTTAAGCCCTCTCACCGTCATCAC CAAAGAGACTGAAAAAGCAGTGCCCCCCAGAGACATTGAAGAAGGCAATGTGAAGATGCTGGGCATGCAG ATCCCCATAAAGAATGTTGAGATGCTGGCCTCTGTTTTGGTTGCCATTAGTGTCACCCTTCTGCTCGTTG TTCTGGCTCTTGGTGGAGTGGTTTGGTACCAACATCGACAGAGAAAGCTACGACGCAATAGGAGGTCCAT CCTGGATGACAGCTTCAAGCTTCTGTCTTTCAAACAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282141
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282141.1, NP_001269070.1
RefSeq Size	3888 bp
RefSeq ORF	2910 bp
Locus ID	9843
UniProt ID	Q9BQS7
Protein Families	Druggable Genome, Transmembrane
Gene Summary	This gene encodes a member of the multicopper oxidase protein family. The encoded protein is involved in the transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system, and may be involved in copper transport and homeostasis. In mouse, defects in this gene can lead to severe microcytic anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (4) contains an alternate 5'-most exon, lacks three consecutive exons in the central coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (d) is shorter, compared to isoform a.

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