Pumilio 2 (PUM2) (NM_001282791) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PUMH2; PUML2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282791, the custom clone sequence may differ by one or more nucleotides ATGTCCCAGCCTATTATGGTACAGAGAAGATCTGGACAGGGTTTTCATGGAAACAGTGAAGTAAATGCAA TACTGTCTCCGCGATCAGAAAGTGGAGGCCTTGGTGTGAGCATGGTAGAATATGTATTAAGTTCTTCTCC TGCTGATAAATTGGATTCTCGATTTAGGAAGGGAAATTTTGGCACTAGAGATGCTGAAACAGATGGACCT GAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAGGACCAAAACAGAGATCTTAAACAAGGAG ATGATGATGATTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAATGGATGCCGATTGCAAAGATTTTAA TCGTACTCCTGGAAGTCGTCAAGCCTCTCCAACTGAAGTAGTTGAGCGCTTGGGCCCCAATACTAATCCC TCAGAAGGACTGGGGCCTCTTCCTAATCCTACAGCTAATAAACCACTTGTTGAAGAATTTTCAAATCCTG AAACTCAGAATCTGGATGCCATGGAACAAGTTGGTCTGGAATCCTTACAGTTTGACTATCCTGGTAATCA GGTACCAATGGACTCTTCAGGAGCTACTGTAGGCCTTTTTGACTACAATTCCCAGCAGCAGCTCTTTCAG AGGACTAATGCACTAACAGTTCAACAGTTAACTGCAGCTCAACAGCAGCAATATGCATTAGCAGCAGCTC AGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTCCAGCTGCATTTGTGCCAAATCCATACAT TATTAGTGCTGCTCCTCCAGGGACCGATCCGTATACTGCAGCAGGATTGGCTGCAGCAGCTACATTAGCA GGTCCAGCAGTGGTTCCACCTCAGTATTACGGCGTTCCATGGGGGGTGTATCCAGCCAACTTATTTCAGC AGCAAGCTGCAGCTGCGGCAAATAACACAGCCAGTCAGCAAGCAGCATCACAAGCTCAGCCTGGACAGCA ACAGGTTCTCCGTGCTGGAGCAGGTCAGCGTCCTCTTACTCCCAATCAGGGTCAGCAAGGGCAGCAAGCA GAATCACTTGCGGCAGCTGCAGCAGCAAATCCAACATTGGCTTTTGGTCAGGGTCTTGCTACTGGCATGC CAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCTTAGTGGTTGGCCCTGGAGC AAGGACTGGCCTTGGAGCTCCAGTTCGGTTAATGGCTCCAACACCTGTTTTAATTAGTTCAGCAGCAGCA CAAGCTGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAGTAGCCTTACAGGCAGCACAAATGGTCTGTTTC GGCCAATTGGCACTCAGCCACCACAGCAGCAGCAACAGCAGCCAAGCACTAATCTGCAATCTAATTCATT TTATGGAAGCAGTTCTTTGACTAATAGCTCCCAGAGTAGTTCTTTATTTTCTCATGGACCTGGTCAACCT GGAAGTACATCTCTTGGCTTTGGAAGTGGTAACTCTTTGGGTGCTGCTATAGGCTCAGCCCTCAGTGGAT TTGGTTCATCAGGAGGACTGACAAATGGTAGTGGTCGATATATCTCTGCAGCACCTGGAGCAGAAGCAAA ATATCGAAGTGCTTCAAGCACTTCCAGTCTATTTAGCTCCAGCAGCCAGCTCTTTCCTCCTTCCCGGCTT CGGTATAATAGGTCTGATATTATGCCTTCTGGCCGCAGTAGATTATTGGAAGATTTCAGAAACAACCGCT TCCCAAACCTTCAGCTTAGAGACTTGATTGGACATATAGTTGAGTTTTCTCAAGACCAGCATGGTTCTAG ATTCATACAGCAAAAACTAGAGAGAGCTACTCCAGCTGAGCGACAGATGGTATTTAATGAAATTCTGCAA GCAGCCTATCAATTAATGACTGATGTTTTTGGCAACTATGTTATACAGAAGTTTTTTGAGTTTGGGAGTC TGGATCAAAAATTAGCCCTGGCTACTCGTATTCGTGGTCATGTTCTACCCTTAGCCTTGCAGATGTATGG CTGCCGCGTTATTCAGAAAGCATTAGAATCTATTTCTTCTGACCAGCAGAGTGAAATGGTAAAGGAGCTG GATGGTCATGTGCTCAAATGTGTGAAAGATCAGAATGGAAACCATGTTGTACAAAAATGTATCGAATGTG TTCAGCCACAGTCACTACAGTTCATCATTGATGCTTTCAAGGGACAAGTATTTGTGCTTTCAACTCATCC TTATGGCTGCAGAGTAATTCAGCGCATCCTAGAGCATTGCACTGCAGAACAGACCTTACCTATCTTAGAA GAACTCCACCAACATACAGAGCAGTTGGTACAGGATCAGTATGGCAATTATGTTATTCAGCATGTACTGG AACACGGTCGACCTGAAGACAAGAGCAAAATTGTTTCCGAAATCAGGGGAAAGGTTTTAGCCCTGAGTCA ACACAAATTTGCCAGCAATGTAGTAGAAAAGTGTGTTACTCATGCCTCCCGTGCTGAGAGAGCTTTACTG ATTGACGAGGTTTGCTGCCAGAATGATGGTCCTCACAGTGCCTTATACACCATGATGAAGGACCAGTATG CCAATTACGTGGTTCAAAAGATGATTGATATGGCTGAACCTGCTCAGAGAAAGATAATCATGCACAAGAT TCGACCTCACATTACTACTTTGCGCAAATACACATACGGGAAGCATATACTGGCCAAGTTGGAAAAGTAT TATTTGAAGAATAGCCCGGACCTAGGACCTATTGGAGGACCACCAAATGGAATGCTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282791
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282791.1, NP_001269720.1
RefSeq Size	6002 bp
RefSeq ORF	2790 bp
Locus ID	23369
Gene Summary	This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) contains a distinct 5' UTR, lacks an in-frame portion of the 5' coding region and lacks an alternate in-frame exon in the coding region compared to variant 1. The resulting isoform (4) has a shorter N-terminus compared to isoform 1.

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