DGKH (NM_001297429) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DGKeta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001297429, the custom clone sequence may differ by one or more nucleotides ATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGACCCACCTTCTGTAACGT GTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCCTGCGAAGGTACACACTGCCTGCAAAGA TTTATACCATCCAATATGTCCACTTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAACAGC ACCGATTCCGATGGTTTCTGTAGAGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATT CTAAGAGTGGAGATAATCAGGGAGTAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGT GTTTGATTTAATGAATGGAGGTCCTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTCCGGATT CTTGTTTGTGGAGGCGATGGAAGTGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTGAATAAAC AGTGTCAGCTGGGAGTGTTGCCTTTGGGTACAGGAAATGACCTTGCCCGAGTTCTTGGCTGGGGAGGTTC ATATGACGATGACACCCAACTTCCTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAAATGTTGGAC AGGTGGAGTATAATGACATATGAACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAG CATCTGAAGAATTTTATATGACGATTTATGAAGACTCAGTTGCAACGCATCTTACAAAAATCCTCAATTC TGATGAACATGCAGTGGTCATATCTTCTGCCAAGACGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAA GTAGAAAAGACGTATGACAAAACCTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAG TCCTAAACGAGAAGCTCGAACAACTGCTGCAGGCTTTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCC TGGCCTCAGCCCTCTCATTGTGGAAGAAGATGCTGTGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGC AAAGAGCAGCTTGGGGATGACGTTACAAAACCTTCCTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGT TGCGGGCAAATAGTTTAAAGAAAGCAGTGAGGCAAGTCATTGAGGAAGCCGGAAAAGTTATGGATGACCC GACAGTTCACCCCTGTGAACCAGCTAATCAGTCCTCTGATTATGACAGCACAGAAACAGATGAATCTAAG GAGGAAGCTAAAGATGATGGTGCCAAAGAATCAATAACTGTTAAAACTGCACCTCGGTCTCCAGATGCCC GGGCAAGTTATGGCCATTCCCAAACTGATTCTGTCCCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAACCT CCCTGTGCTCAATACCAGAATAATCTGCCCAGGTTTAAGAGCAGGACTGGCTGCCTCAATTGCTGGGAGT TCGATTATCAACAAAATGTTACTGGCAAACATTGATCCTTTTGGTGCCACGCCGTTTATTGACCCGGATC TAGATTCCGTAGATGGATATTCAGAAAAATGTGTCATGAACAATTACTTTGGGATTGGATTAGATGCAAA AATTTCATTAGAATTTAATAATAAAAGAGAGGAGCACCCTGAAAAATGCAGGAGCCGAACTAAAAACTTG ATGTGGTATGGAGTCCTTGGAACCCGGGAGTTATTACAGAGATCGTACAAGAATTTAGAACAAAGGGTTC AACTTGAGTGTGATGGGCAGTATATTCCTCTTCCCAGCTTGCAAGGCATAGCCGTGTTGAACATTCCCAG CTATGCTGGAGGCACTAACTTTTGGGGTGGAACTAAAGAGGATGATATATTTGCTGCACCATCCTTTGAT GACAAGATCCTGGAAGTTGTAGCAATATTTGATAGCATGCAAATGGCAGTTTCAAGGGTCATTAAACTGC AGCATCATCGAATAGCCCAGTGCCGTACAGTGAAAATCACTATATTTGGTGACGAAGGAGTCCCAGTGCA AGTGGATGGTGAAGCGTGGGTTCAGCCTCCAGGGATTATCAAAATTGTGCACAAAAACAGAGCACAAATG CTAACAAGGGACAGAGCCTTTGAGAGCACTCTGAAATCTTGGGAAGATAAGCAGAAGTGTGATTCTGGTA AACCAGTTCTCCGAACCCATTTGTACATCCATCACGCCATTGACTTGGCAACAGAAGAGGTGTCGCAGAT GCAGCTATGCTCCCAGGCTGCAGAGGAGCTCATTACTAGGATATGTGACGCAGCCACAATTCACTGTCTT TTGGAGCAAGAACTGGCCCATGCTGTGAATGCCTGCTCCCATGCCCTGAATAAAGCCAACCCAAGGTGCC CGGAGAGTCTTACAAGAGACACTGCCACTGAAATAGCCATCAATGTGAAGGCGCTGTATAATGAAACAGA ATCTTTGCTAGTTGGCAGGGTTCCTTTGCAGCTGGAATCGCCACATGAAGAGCGAGTATCCAATGCCTTA CACTCTGTGGAGGTGGAATTACAGAAACTGACAGAGATTCCTTGGCTTTATTATATCTTACACCCAAATG AGGATGAGGAACCTCCTATGGATTGCACCAAAAGGAACAACAGAAGCACCGTATTTCGAATAGTGCCAAA GTTTAAAAAGGAAAAGGTTCAGAAGCAGAAGACAAGTTCACAGCCTGGATCTGGGGATACCGAAAGTGGG TCATGTGAAGCGAATTCTCCAGGGAATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001297429
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297429.1, NP_001284358.1
RefSeq Size	16746 bp
RefSeq ORF	2760 bp
Locus ID	160851
UniProt ID	Q86XP1
Protein Families	Druggable Genome
Protein Pathways	Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Gene Summary	This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, two exons in the central coding region, and an in-frame exon in the 3' coding region, compared to variant 1. It initiates translation at an alternate downstream start codon. The encoded isoform (5) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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