LIMCH1 (NM_001289124) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LIMCH1A; LMO7B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289124, the custom clone sequence may differ by one or more nucleotides ATGCGAAAGGACACTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATCGACTGCT GGGATTCCGAGCGCAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTCCTTCGACAGCCTGGA TTCCTTTGGCTCTCGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGA GGAAGCGACTCTGAATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCAC GGCGGACTTCCCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCA GACGGCCTACGTCCCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGT ACCGCCACCTCCCCGCTGGGTGGGGAGAGGCCCTTCAGGAGCACCAGCATGTTTGACATGCGGTGTGAGG AGGAGGCCGCGGTGCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAG GGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGTTGGAAGAGTCGTAGAAGAAGTGTTTCTCAGGAC TTAATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGTTACTGGCTGGAGAAGATGGGACAAGTGAAC GAAGGAAAAGCATCAAAACCTACAGAGAAATTGTTCAAGAAAAAGAGCGGAGAGAGAGAGAGCTGCATGA AGCATATAAGAACGCTCGGTCCCAGGAGGAGGCAGAGGGGATCCTTCAACAGTACATTGAGAGGTTCACC ATCAGTGAGGCTGTTCTCGAACGCTTGGAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACAGAGCCAA ATTTATCCTCCTTCCTGAATGACCCCAATCCCATGAAATACCTGCGGCAACAGTCACTGCCTCCACCCAA ATTCACTGCCACTGTTGAAACCACCATTGCTCGTGCCAGTGTTCTGGATACCAGCATGTCAGCAGGCAGT GGGTCTCCAAGCAAAACTGTCACTCCCAAAGCAGTGCCTATGCTGACACCCAAGCCTTACTCCCAGCCCA AAAATTCTCAAGATGTTCTGAAGACCTTTAAGGTAGACGGGAAAGTCAGTGTGAATGGAGAGACGGTTCA TAGAGAGGAGGAGAAGGAAAGAGAGTGTCCCACGGTGGCACCTGCCCACTCCTTAACCAAATCCCAGATG TTTGAAGGTGTGGCCAGAGTGCACGGGTCTCCACTGGAGCTGAAACAAGACAACGGTAGCATCGAGATCA ACATAAAGAAGCCAAACTCTGTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACGGAACCGAATAGTCA AGAGGACAAGAATGATGGTGGAAAATCAAGAAAAGGGAATATAGAACTTGCCTCATCAGAACCACAGCAT TTTACAACAACTGTGACTCGATGCAGCCCGACCGTGGCCTTTGTGGAATTTCCCTCCAGCCCCCAGCTGA AGAATGATGTGTCGGAAGAAAAAGACCAGAAGAAACCAGAAAATGAAATGAGTGGAAAGGTGGAGTTGGT GCTGTCACAAAAGGTGGTAAAGCCAAAATCTCCAGAACCCGAAGCAACGCTGACATTTCCATTTCTGGAC AAAATGCCTGAAGCCAACCAACTACATTTGCCAAATCTCAATTCTCAAGAGTCGCCTGGCACTGCCAGTG TTCCGCTTAGAGTTCAGAACTCTTGGCGACGATCCCAGTTTTTCTCCCAGTCAGTGGATTCTCCAAGCAG TGAGAAGTCACCTGTTATGACACCTTTTAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCAGGCGACAG GAAAAATGGCAACAGGAACAGGAACGTTTGCTCCAGGAGAGATACCAGAAGGAGCAGGACAAGCTGAAAG AAGAGTGGGAAAAGGCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGATACTATGAGGAGGAGCGTAAGAT AATTGAAGACACTGTGGTTCCATTTACTGTTTCTTCAAGTTCCGCTGACCAGCTGTCTACCTCTTCCTCC ATGACTGAAGGCAGTGGGACAATGAATAAGATAGACCTGGGAAACTGTCAAGATGAAAAACAAGACAGAA GATGGAAGAAATCATTCCAGGGAGATGACAGTGACTTATTGCTGAAGACTAGGGAAAGTGATCGACTGGA GGAGAAGGGCAGCCTAACTGAAGGGGCCTTGGCTCATTCTGGGAACCCTGTATCAAAAGGAGTCCATGAA GACCATCAGCTGGATACCGAGGCTGGGGCCCCACACTGTGGAACAAACCCACAGCTTGCTCAGGATCCAT CCCAGAATCAGCAGACATCAAATCCAACGCACAGTTCAGAAGATGTGAAGCCAAAAACCCTCCCGCTGGA TAAAAGCATTAACCATCAGATCGAGTCTCCCAGTGAAAGGCGGAAGTCTATAAGTGGAAAGAAGCTGTGC TCTTCCTGTGGGCTTCCTTTGGGTAAAGGAGCTGCAATGATCATCGAGACCCTCAATCTCTATTTTCACA TCCAGTGTTTCAGGTGTGGAATTTGTAAAGGCCAGCTTGGAGATGCAGTGAGTGGGACGGATGTTAGGAT TCGAAATGGTCTCCTGAACTGTAATGATTGCTACATGCGATCCAGAAGTGCCGGGCAGCCTACAACATTG TGA
Restriction Sites	SgfI-MluI
ACCN	NM_001289124
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289124.1, NP_001276053.1
RefSeq Size	6187 bp
RefSeq ORF	2733 bp
Locus ID	22998
UniProt ID	Q9UPQ0
Gene Summary	Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function]

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