NFATC4 (NM_001288802) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NF-AT3; NF-ATC4; NFAT3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288802, the custom clone sequence may differ by one or more nucleotides ATGCCTGCTTCAATCTCCTCCATCTTCCCAGGTCCAACTCTGCTTTTGTCTTGTGGCTCAGAAGAACTGG ACTCAGAGGATGCCCCGCCATGCTGCCGTCTGGCCTTGGGAGAGCCCCCTCCCTATGGCGCTGCACCTAT CGGTATTCCCCGACCTCCACCCCCTCGGCCTGGCATGCATTCGCCACCGCCGCGACCAGCCCCCTCACCT GGCACCTGGGAGAGCCAGCCCGCCAGGTCGGTGAGGCTGGGAGGACCAGGAGGGGGTGCTGGGGGTGCTG GGGGTGGCCGTGTTCTCGAGTGTCCCAGCATCCGCATCACCTCCATCTCTCCCACGCCGGAGCCGCCAGC AGCGCTGGAGGACAACCCTGATGCCTGGGGGGACGGCTCTCCTAGAGATTACCCCCCACCAGAAGGCTTT GGGGGCTACAGAGAAGCAGGGGGCCAGGGTGGGGGGGCCTTCTTCAGCCCAAGCCCTGGCAGCAGCAGCC TGTCCTCGTGGAGCTTCTTCTCCGATGCCTCTGACGAGGCAGCCCTGTATGCAGCCTGCGACGAGGTGGA GTCTGAGCTAAATGAGGCGGCCTCCCGCTTTGGCCTGGGCTCCCCGCTGCCCTCGCCCCGGGCCTCCCCT CGGCCATGGACCCCCGAAGATCCCTGGAGCCTGTATGGTCCAAGCCCCGGAGGCCGAGGGCCAGAGGATA GCTGGCTACTCCTCAGTGCTCCTGGGCCCACCCCAGCCTCCCCGCGGCCTGCCTCTCCATGTGGCAAGCG GCGCTATTCCAGCTCGGGAACCCCATCTTCAGCCTCCCCAGCTCTGTCCCGCCGTGGCAGCCTGGGGGAA GAGGGGTCTGAGCCACCTCCACCACCCCCATTGCCTCTGGCCCGGGACCCGGGCTCCCCTGGTCCCTTTG ACTATGTGGGGGCCCCACCAGCTGAGAGCATCCCTCAGAAGACACGGCGGACTTCCAGCGAGCAGGCAGT GGCTCTGCCTCGGTCTGAGGAGCCTGCCTCATGCAATGGGAAGCTGCCCTTGGGAGCAGAGGAGTCTGTG GCTCCTCCAGGAGGTTCCCGGAAGGAGGTGGCTGGCATGGACTACCTGGCAGTGCCCTCCCCACTCGCTT GGTCCAAGGCCCGGATTGGGGGACACAGCCCTATCTTCAGGACCTCTGCCCTACCCCCACTGGACTGGCC TCTGCCCAGCCAATATGAGCAGCTGGAGCTGAGGATCGAGGTACAGCCTAGAGCCCACCACCGGGCCCAC TATGAGACAGAAGGCAGCCGTGGAGCTGTCAAAGCTGCCCCTGGCGGTCACCCCGTAGTCAAGCTCCTAG GCTACAGTGAGAAGCCACTGACCCTACAGATGTTCATCGGCACTGCAGATGAAAGGAACCTGCGGCCTCA TGCCTTCTATCAGGTGCACCGTATCACAGGCAAGATGGTGGCCACGGCCAGCTATGAAGCCGTAGTCAGT GGCACCAAGGTGTTGGAGATGACTCTGCTGCCTGAGAACAACATGGCGGCCAACATTGACTGCGCGGGAA TCCTGAAGCTTCGGAATTCAGACATTGAGCTTCGGAAGGGTGAGACGGACATCGGGCGCAAAAACACACG TGTACGGCTGGTGTTCCGGGTACACGTGCCCCAGGGCGGCGGGAAGGTCGTCTCAGTACAGGCAGCATCG GTGCCCATCGAGTGCTCCCAGCGCTCAGCCCAGGAGCTGCCCCAGGTGGAGGCCTACAGCCCCAGTGCCT GCTCTGTGAGAGGAGGCGAGGAACTGGTACTGACTGGCTCCAACTTCCTGCCAGACTCCAAGGTGGTGTT CATTGAGAGGGGTCCTGATGGGAAGCTGCAATGGGAGGAGGAGGCCACAGTGAACCGACTGCAGAGCAAC GAGGTGACGCTGACCCTGACTGTCCCCGAGTACAGCAACAAGAGGGTTTCCCGGCCAGTCCAGGTCTACT TTTATGTCTCCAATGGGCGGAGGAAACGCAGTCCTACCCAGAGTTTCAGGTTTCTGCCTGTGATCTGCAA AGAGGAGCCCCTACCGGACTCATCTCTGCGGGGTTTCCCTTCAGCATCGGCAACCCCCTTTGGCACTGAC ATGGACTTCTCACCACCCAGGCCCCCCTACCCCTCCTATCCCCATGAAGACCCTGCTTGCGAAACTCCTT ACCTATCAGAAGGCTTCGGCTATGGCATGCCCCCTCTGTACCCCCAGACGGGGCCCCCACCATCCTACAG ACCGGGCCTGCGGATGTTCCCTGAGACTAGGGGTACCACAGGTTGTGCCCAACCACCTGCAGTTTCCTTC CTTCCCCGCCCCTTCCCTAGTGACCCGTATGGAGGGCGGGGCTCCTCTTTCTCCCTGGGGCTGCCATTCT CTCCGCCAGCCCCCTTTCGGCCGCCTCCTCTTCCTGCATCCCCACCGCTTGAAGGCCCCTTCCCTTCCCA GAGTGATGTGCATCCCCTACCTGCTGAGGGATACAATAAGGTAGGGCCAGGCTATGGCCCTGGGGAGGGG GCTCCGGAGCAGGAGAAATCCAGGGGTGGCTACAGCAGCGGCTTCCGAGACAGTGTCCCTATCCAGGGTA TCACGCTGGAGGAAGGTGGGTGTGGGACTGGGGGCTGTGAGTGTGAGTGTGTGCAAGAGATTGCTCTGCA TGTTTGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001288802
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288802.1, NP_001275731.1
RefSeq Size	5538 bp
RefSeq ORF	2670 bp
Locus ID	4776
UniProt ID	Q14934
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
Gene Summary	This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (6) lacks part of the 5' coding region, contains an alternate 5'-terminal exon, and initiates translation from an alternate start codon, compared to variant 1. The encoded isoform (6) has a shorter and distinct N-terminus, compared to isoform 1.

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