TOP3B (NM_001282113) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TOP3B1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282113, the custom clone sequence may differ by one or more nucleotides ATGAAGACTGTGCTCATGGTTGCTGAAAAGCCGTCCTTGGCACAGTCAATTGCCAAAATCCTCTCTAGAG GGAGCCTGTCCTCACACAAAGGGCTGAACGGGGCCTGCTCAGTCCACGAGTACACTGGGACCTTTGCTGG CCAGCCAGTGCGCTTCAAGATGACGTCTGTCTGTGGTCACGTGATGACCCTGGATTTCCTGGGAAAATAC AACAAATGGGACAAAGTGGACCCCGCAGAACTGTTCAGCCAAGCTCCCACGGAGAAGAAAGAAGCTAACC CCAAGCTGAACATGGTGAAGTTCCTGCAGGTGGAGGGCAGAGGCTGCGACTACATCGTGCTGTGGCTGGA CTGCGACAAGGAGGGGGAGAACATCTGCTTTGAGGTTCTTGATGCTGTTCTGCCCGTCATGAACAAGGCC CATGGTGGCGAGAAGACCGTGTTCCGGGCCAGGTTTAGCTCCATCACGGACACAGACATCTGTAATGCCA TGGCCTGCCTAGGCGAGCCTGACCACAACGAGGCGCTCTCAGTGGATGCTCGCCAGGAGCTGGACCTGCG AATCGGCTGTGCATTCACCAGGTTTCAGACTAAATATTTCCAGGGGAAATACGGTGATTTAGACAGCTCT CTCATCTCCTTTGGGCCGTGTCAGACTCCAACCCTGGGATTCTGTGTGGAGAGACATGATAAAATCCAGT CCTTCAAACCAGAGACCTACTGGGTGCTGCAGGCCAAGGTTAACACTGACAAAGACAGATCTCTCCTTTT GGACTGGGACCGAGTAAGAGTGTTTGACCGGGAGATCGCACAGATGTTTTTAAACATGACAAAGCTGGAG AAGGAAGCCCAGGTGGAGGCCACAAGCAGGAAAGAAAAGGCCAAGCAGAGGCCCCTGGCCCTGAACACTG TGGAGATGCTGCGTGTGGCCAGCTCTTCTCTGGGCATGGGGCCGCAGCACGCCATGCAGACGGCTGAGCG GCTCTACACGCAAGGCTACATCAGCTACCCACGGACAGAGACCACCCACTACCCTGAGAACTTTGACCTG AAGGGCTCTCTGCGGCAGCAGGCCAACCACCCCTACTGGGCCGACACGGTGAAGCGGTTGTTAGCAGAAG GTATCAACCGCCCGCGGAAAGGCCATGACGCCGGCGACCATCCCCCCATCACCCCCATGAAGTCTGCCAC AGAGGCCGAATTAGGGGGTGACGCGTGGCGGCTCTATGAGTACATCACCAGACACTTCATCGCCACGGTC AGCCATGACTGCAAGTACCTGCAGAGCACCATCTCCTTCAGAATTGGGCCCGAGCTCTTCACCTGCTCCG GGAAGACCGTCCTCTCACCAGGCTTCACGGAGGTCATGCCCTGGCAGAGCGTGCCCCTGGAGGAGAGCCT GCCCACTTGCCAGCGGGGTGATGCCTTCCCTGTGGGCGAGGTGAAGATGCTGGAGAAGCAGACGAACCCA CCCGACTACCTGACGGAGGCCGAGCTCATCACGCTCATGGAGAAGCATGGCATCGGCACGGATGCCAGCA TCCCTGTGCATATCAACAACATCTGCCAGCGCAACTATGTCACGGTGGAGAGCGGGCGCCGGCTCAAGCC CACCAACCTCGGCATCGTCCTGGTGCACGGCTACTATAAGATTGATGCAGAGCTGGTGCTCCCCACCATC CGCAGTGCAGTGGAGAAGCAGCTGAACCTGATCGCCCAGGGCAAGGCCGACTACCGCCAGGTCCTGGGCC ACACCCTGGACGTGTTCAAGAGGAAGTTCCACTACTTTGTCGACTCCATTGCTGGCATGGATGAGTTGAT GGAGGTGTCTTTCTCGCCCCTGGCGGCCACAGGCAAGCCCCTCTCACGCTGTGGGAAGTGCCACCGCTTC ATGAAGTACATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCCCACTGCGATGAGACCTACACGCTCCCCC AGAACGGCACCATCAAGCTCTACAAGGAGCTCCGCTGCCCTCTGGATGACTTCGAGCTGGTCCTGTGGTC ATCAGGCTCTCGGGGCAAGAGCTACCCGCTGTGCCCCTACTGCTACAACCACCCACCCTTCCGAGACATG AAGAAAGGCATGGGCTGCAACGAGTGTACGCACCCCTCCTGCCAGCACTCGCTGAGCATGCTGGGCATCG GCCAGTGCGTGGAATGTGAGAGCGGGGTGCTGGTGCTGGACCCCACCTCGGGCCCCAAGTGGAAGGTGGC CTGCAACAAGTGCAACGTGGTAGCGCACTGCTTCGAGAACGCCCACCGCGTGCGGGTGTCCGCCGACACC TGCAGTGTCTGTGAGGCCGCCTTGCTTGATGTGGACTTCAACAAGGCCAAGTCCCCACTCCCGGGCGATG AGACGCAGCACATGGGCTGCGTCTTTTGTGACCCCGTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCGGC CTCCTGCCACCCCATGCACCGCGGTGGACCAGGGAGAAGGCAGGGTCGAGGGCGGGGCCGGGCCAGGAGG CCCCCTGGGAAGCCCAACCCCAGACGGCCCAAGGACAAGATGTCAGCCCTGGCCGCCTACTTTGTATGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282113
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282113.1, NP_001269042.1
RefSeq Size	2901 bp
RefSeq ORF	2589 bp
Locus ID	8940
UniProt ID	O95985
Protein Pathways	Homologous recombination
Gene Summary	This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) contains an alternate 5' terminal exon and uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1-5 encode the same protein (isoform 1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.