alpha 1 Catenin (CTNNA1) (NM_001290309) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAP102; MDPT2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290309, the custom clone sequence may differ by one or more nucleotides ATGAAGGCTGCTGCAGGAGAGTTCGCAGATGATCCCTGCTCTTCTGTGAAGCGAGGCAACATGGTTCGGG CAGCTCGAGCTTTGCTCTCTGCTGTTACCCGGTTGCTGATTTTGGCTGACATGGCAGATGTCTACAAATT ACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATCTTGAAGTTGAGGAATGCTGGCAATGAACAAGACTTA GGAATCCAGTATAAAGCCCTAAAACCTGAAGTGGATAAGCTGAACATTATGGCAGCCAAAAGACAACAGG AATTGAAAGATGTTGGCCATCGTGATCAGATGGCTGCAGCTAGAGGAATCCTGCAGAAGAACGTTCCGAT CCTCTATACTGCATCCCAGGCATGCCTACAGCACCCTGATGTCGCAGCCTATAAGGCCAACAGGGACCTG ATATACAAGCAGCTGCAGCAGGCGGTCACAGGCATTTCCAATGCAGCCCAGGCCACTGCCTCAGACGATG CCTCACAGCACCAGGGTGGAGGAGGAGGAGAACTGGCATATGCACTCAATAACTTTGACAAACAAATCAT TGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCTTCCCTGGAGGAGCGTCTGGAAAGCATCATT AGTGGGGCTGCCTTGATGGCCGACTCGTCCTGCACGCGTGATGACCGTCGTGAGCGAATTGTGGCAGAGT GTAATGCTGTCCGCCAGGCCCTGCAGGACCTGCTTTCGGAGTACATGGGCAATGCTGGACGTAAAGAAAG AAGTGATGCACTCAATTCTGCAATAGATAAAATGACCAAGAAGACCAGGGACTTGCGTAGACAGCTCCGC AAAGCTGTCATGGACCACGTTTCAGATTCTTTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAG CTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATT GATTGAGGTTGCCAACTTGGCCTGTTCCATCTCAAATAATGAAGAAGGTGTAAAGCTTGTTCGAATGTCT GCAAGCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAACCACAGA GTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGA TGCTGTCGATGACATTACTTCCATTGATGACTTCTTGGCTGTCTCAGAGAATCACATTTTGGAAGATGTG AACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTGGATGGCCTGGACCGCACAGCTGGTGCAATTCGAG GCCGGGCAGCCCGGGTCATTCACGTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGA GAAGGTTCTGGAAGCCACTAAGCTGCTCTCCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCA GCCGTGGAAGCCCTCAGCTCGGACCCTGCCCAGCCCATGGATGAGAATGAGTTTATCGATGCTTCCCGCC TGGTATATGATGGCATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGA CTCTGACTTTGAGACAGAAGATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTG ATAGCTGGCCAGAGTGCCCGGGCGATCATGGCTCAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAAC AGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAA TGACATCATTGTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAA GGACCACTCAAAAATACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCAGGATGG ACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAGGACCTGCTGGCCTACCT GCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTC GGCGGGGAGCTTGTTGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCAAGAACTTGATGA ATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCTACCAAATACCAAAAGTCACAGGGTATGGC TTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAG AAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAGGCCC TCAGCGAGTTCAAAGCTATGGACAGCATCTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290309
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290309.2, NP_001277238.1
RefSeq Size	3605 bp
RefSeq ORF	2412 bp
Locus ID	1495
UniProt ID	P35221
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction
Gene Summary	This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016] Transcript Variant: This variant (3) lacks an alternate exon in the 5' end compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (3) with a shorter N-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.