H6PD (NM_001282587) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CORTRD1; G6PDH; GDH; H6PDH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282587, the custom clone sequence may differ by one or more nucleotides ATGTTAGCAGAGCCTTTTAACTGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGCTTGGCCC TTCTGGGCTGCCTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTGCTGGGAGCAACTGGGGA CCTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTACCTGGATGAAGCGGGGAGGGGTCACAGT TTTAGCTTCCATGGAGCTGCTCTGACAGCCCCCAAGCAGGGTCAAGAGCTCATGGCCAAGGCCCTGGAAT CCCTCTCCTGCCCCAAGGACATGGCACCCAGTCACTGTGCAGAGCACAAGGATCAGTTCCTGCAGCTGAG CCAGTACCGCCAACTGAAGACGGCCGAGGACTATCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAG CACGCAGGCCTCCGGGAGGCTGGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAAGACATTG CCCGCAACATCAACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTTGTCCTTGAGAAACCCTT TGGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAACTCGGGACCTTTTTCCAGGAGGAGGAGATG TACCGGGTGGACCATTACTTAGGCAAGCAGGCTGTGGCGCAGATCCTGCCTTTCCGAGACCAGAACCGCA AGGCTTTGGACGGCCTCTGGAACCGGCACCATGTGGAGCGGGTGGAGATCATCATGAAAGAGACCGTGGA TGCTGAAGGCCGCACCAGCTTCTATGAGGAGTACGGTGTCATTCGCGACGTCCTCCAGAACCATCTGACG GAGGTCCTCACCCTCGTGGCCATGGAGCTGCCCCACAATGTCAGCAGTGCGGAGGCTGTGCTGCGGCACA AGCTTCAGGTCTTCCAGGCGCTGCGGGGCCTGCAGAGGGGCAGTGCCGTCGTGGGCCAGTACCAGTCTTA CAGTGAGCAGGTGCGCAGAGAGCTGCAGAAGCCAGACAGCTTCCACAGCCTGACGCCGACCTTCGCAGCC GTCCTAGTGCACATTGACAACCTTCGCTGGGAGGGCGTGCCTTTCATCCTGATGTCTGGCAAAGCCTTGG ACGAGAGAGTGGGCTACGCTCGGATCTTGTTCAAGAACCAGGCCTGCTGTGTGCAGAGCGAAAAGCACTG GGCCGCGGCGCAGAGCCAGTGCCTGCCCCGGCAGCTCGTCTTCCACATCGGCCATGGCGACCTGGGCAGC CCTGCCGTGCTGGTCAGCAGGAACCTGTTCAGGCCCTCCCTGCCCTCCAGCTGGAAGGAAATGGAGGGAC CACCTGGGCTCCGCCTTTTCGGCAGCCCTCTGTCCGATTACTACGCCTACAGCCCTGTGCGGGAGCGGGA CGCCCACTCCGTCCTCTTATCCCATATCTTCCATGGCCGGAAGAATTTCTTCATCACCACAGAGAACTTG CTGGCCTCCTGGAACTTCTGGACCCCTCTGCTGGAGAGCCTGGCCCATAAGGCCCCACGCCTCTACCCTG GAGGAGCTGAGAATGGCCGTCTGTTGGACTTTGAGTTCAGTAGCGGCCGGTTGTTCTTTTCCCAGCAGCA GCCGGAGCAGCTGGTGCCAGGGCCAGGGCCGGCCCCAATGCCCAGTGACTTCCAGGTCCTCAGGGCCAAG TACCGAGAGAGCCCGCTGGTCTCCGCCTGGTCCGAGGAGCTGATCTCTAAGCTGGCTAATGACATCGAGG CCACCGCTGTGCGAGCCGTGCGGCGCTTTGGCCAGTTCCACCTGGCACTGTCGGGGGGCTCGAGCCCCGT GGCCCTGTTCCAGCAGCTGGCCACGGCGCACTATGGCTTCCCCTGGGCCCACACGCACCTGTGGCTGGTT GACGAGCGCTGCGTCCCACTCTCAGACCCGGAGTCCAACTTCCAGGGCCTGCAGGCCCACCTGCTGCAGC ACGTCCGGATCCCCTACTACAACATCCACCCCATGCCTGTGCACCTGCAGCAGCGGCTCTGCGCCGAGGA GGACCAGGGCGCCCAGATCTATGCCAGGGAGATCTCAGCCCTGGTGGCCAACAGCAGCTTCGACCTGGTG CTGCTGGGCATGGGTGCCGACGGGCACACAGCCTCCCTCTTCCCACAGTCACCCACTGGCCTGGATGGCG AGCAGCTGGTCGTGCTGACCACGAGCCCCTCCCAGCCACACCGCCGCATGAGCCTTAGCCTGCCTCTCAT CAACCGCGCCAAGAAGGTGGCAGTCCTGGTCATGGGCAGGATGAAGCGTGAGATCACCACGCTGGTGAGC CGGGTGGGCCATGAGCCCAAGAAGTGGCCCATCTCGGGTGTCCTGCCGCACTCCGGCCAGCTGGTGTGGT ACATGGACTACGACGCCTTCCTGGGATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282587
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282587.1, NP_001269516.1
RefSeq Size	9043 bp
RefSeq ORF	2409 bp
Locus ID	9563
UniProt ID	O95479
Protein Pathways	Metabolic pathways, Pentose phosphate pathway
Gene Summary	There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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