MRTFA (NM_001282662) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BSAC; MAL; MKL; MKL1; MRTF-A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282662, the custom clone sequence may differ by one or more nucleotides ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCCAGGACAGAGG ACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTCAGGATGCACATTTTGGAAGA GACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGACTAGCCGATGACCTC AATGAGAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAGTCCA GCCTGAAGGAAGCCATCATTGTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTCGATGA GGACAGCAGCGATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCGTCACCC CTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGGTTGTGTCTCAAC TTCCGATGGGCCGGGATTCCAGAGAAATGCTTTTCCTGGCAGAGCAGCCTCCTCTGCCTCCCCCACCTCT GCTGCCTCCCAGCCTCACCAATGGAACCACTATCCCCACTGCCAAGTCCACCCCCACACTCATTAAGCAA AGCCAACCCAAGTCTGCCAGTGAGAAGTCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGA AGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCCCCCATGGACTC ATCCTACGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAACCAGCAGCAGCAG CAGCACCACAACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCCCTGGGAAGCAGCG GGACCCCCCCAGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCGGGCGCCCCTGGGCCCTGTGG GCTGGCACGTCAGAACAGCACCTCACTGACTGGCAAGCCGGGAGCCCTGCCGGCCAACCTGGACGACATG AAGGTGGCAGAGCTGAAGCAGGAGCTGAAGTTGCGATCACTGCCTGTCTCGGGCACCAAAACTGAGCTGA TTGAGCGCCTTCGAGCCTATCAAGACCAAATCAGCCCTGTGCCAGGAGCCCCCAAGGCCCCTGCCGCCAC CTCTATCCTGCACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGGCTGAGCACGGGGCCAGCC CTGGTGGCAGCAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCCAGCAGTGGGGTGGTGAAGT TTGGCAGCACGGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGCTCACTGCTCAGCACGGGCGA TGAAAACTCCACCCCCGGGGACACCTTTGGTGAGATGGTGACATCACCTCTGACGCAGCTGACCCTGCAG GCCTCGCCACTGCAGATCCTCGTGAAGGAGGAGGGCCCCCGGGCCGGGTCCTGTTGCCTGAGCCCTGGGG GGCGGGCGGAGCTAGAGGGGCGCGACAAGGACCAGATGCTGCAGGAGAAAGACAAGCAGATCGAGGCGCT GACGCGCATGCTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAGCTGCAGCTGGAGCAGGAGAAGCGA GCCCAGCAGCCCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTGAAGCAGGAGAACAGCTTCTCCAGCT GCCAGCTGAGCCAGCAGCCCCTGGGCCCCGCTCACCCATTCAACCCCAGCCTGGCGGCCCCAGCCACCAA CCACATAGACCCTTGTGCTGTGGCCCCGGGGCCCCCGTCCGTGGTGGTGAAGCAGGAAGCCTTGCAGCCT GAGCCCGAGCCGGTCCCCGCCCCCCAGTTGCTTCTGGGGCCTCAGGGCCCCAGCCTCATCAAGGGGGTTG CACCTCCCACCCTCATCACCGACTCCACAGGGACCCACCTTGTCCTCACCGTGACCAATAAGAATGCAGA CAGCCCTGGCCTGTCCAGTGGGAGCCCCCAGCAGCCCTCGTCCCAGCCTGGCTCTCCAGCGCCTGCCCCC TCTGCCCAGATGGACCTGGAGCACCCACTGCAGCCCCTCTTTGGGACCCCCACTTCTCTGCTGAAGAAGG AACCACCTGGCTATGAGGAAGCCATGAGCCAGCAGCCCAAACAGCAGGAAAATGGTTCCTCAAGCCAGCA GATGGACGACCTGTTTGACATTCTCATTCAGAGCGGAGGTAACCCCAGAAATTTCAGCAGATTTCAAGGA GCCGCCATCCCTGCCAGGGAAGGAGAAGCCATCCCCGAAGACAGTCTGTGGGTCCCCCCTGGCAGCACAG CCATCACCTTCTGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282662
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282662.1, NP_001269591.1
RefSeq Size	4560 bp
RefSeq ORF	2397 bp
Locus ID	57591
Protein Families	Transcription Factors
Gene Summary	The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, which results in a frameshift and an early stop codon compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus compared to isoform 5. CCDS Note: The coding region has been updated to start at a non-AUG translation start codon that is supported by publications and conservation.

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