HERC4 (NM_001278186) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278186, the custom clone sequence may differ by one or more nucleotides ATGATGAAAATGGAAGGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTA CCAGTCATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCTTGTGG ACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGGCTTGGTGGTAATGGG CAGCTGGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGGTACCCCTATA ATGGGCAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAAAAGAATTTTCTCAGGGGGAGA TCAAAGCTTTTCACATTACTCTAGTCCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCG ACAAAGCAGATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTC CTGTGGAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTTTTAGC TGTTAGCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCTT TTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGA ATCTTATTCCTAAACTGACTAGCTCCTTACCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGA ATGTCCCCTGATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAAC CTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGA TAGTAGAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTGA AAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTACATAGGGTAAATGAG AAAATGGGACAGATTATACAGTATGATAAATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAA ATGATTATATCAACTGGGTCCAACAGCAGGCCTATGGAATGTTGGCAGATATCCCTGTTACAATCTGTAC ATATCCATTTGTATTTGATGCCCAAGCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAG ATGGCTATTGATCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATC CCTGCTTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAAACAAA GAACATAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTGGATGCAGGAGGGGTG CGCAAAGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCTAAATACGGCATGTTTAGGTATTATG AAGATTCCAGGCTCATTTGGTTTTCTGATAAGACATTTGAAGACAGTGATTTGTTCCATTTGATTGGTGT TATCTGTGGCTTAGCAATTTATAATTGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAAA CTACTGAAAAAGAAGCCATCCTTGGATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAAC AGTTACTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTACGATCACAGTTGAAAA CTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTTAACAAACAAAATCGG CAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTATTTGATGCTTTTC ATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTCCTTCTGCTCTTTCAGCCTAATGAACTACAAGCAAT GGTCATTGGAAATACAAATTATGATTGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGG GCAGAACATCCTACGATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGT TTCTGTTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCA GTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTTCTGGATCTTCCAAAA TATACAGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAA TATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278186
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278186.1, NP_001265115.1
RefSeq Size	4255 bp
RefSeq ORF	2385 bp
Locus ID	26091
UniProt ID	Q5GLZ8
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (4) lacks two alternate exons compared to variant 1, and one causes a frameshift. These differences cause translation initiation at a downstream AUG and result in an isoform (d) with a shorter and distinct N-terminus and lacking an alternate internal segment, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.