Epac2 (RAPGEF4) (NM_001282901) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282901, the custom clone sequence may differ by one or more nucleotides ATGATAAGAGATAGAAAATACCACCTAAAGACATACAGACAATGCTGTGTGGGAACTGAACTGGTGGACT GGATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCAAGCTGTTGGCATGTGGCAAGTCCTGTTAGA AGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTCCAAGACAAATATTTATTCTATCGATTTCTG GATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGGAGAAGAAGGAGTGTGATGAGGAGCTCCAGG ACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCACATGAGGATGATCCTTCGCAAACCACCTGG CCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAGCTTCTTCATATTAAAGCCTTATCCCATCTT TCTACCACAGTGAAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGGACTGTGT TGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTATTCTAAAAGGATCAGTGAATGTAGTCATTTA CGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTAGCACTAGTGAATGATGCC CCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCCATTTCTTAAGAGTAGACAAGGAGGATTTCA ACCGGATCCTAAGGGACGTGGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTGCT GGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTATACTGTG ATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGGCAACTTTAAATG AAGCAACAGATTCTGTTTTAAATGACTTTATTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTG CCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAAGGTACAGAACAGGAGAAAATGGATTATGCC CTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGTGGGCTGCCATGTATGGAGACCTCCTGCAAG AGGATGACGTGTCTATGGCCTTCCTGGAGGAGTTTTATGTATCTGTATCAGATGATGCCCGGATGATTGC TGCCCTCAAGGAGCAACTGCCAGAGTTGGAGAAGATTGTCAAGCAAATCTCAGAAGATGCAAAGGCACCA CAAAAGAAGCACAAGGTTCTTTTGCAACAGTTCAATACGGGCGATGAGAGAGCCCAGAAGCGCCAGCCTA TCCGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCATGGACCACACCTACACAACCATTCGGGTGCC AGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCCGACAAGCTGGGCTCCGGGGAGGGCCTGATC ATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCAAACCTAATGATGTTTCAGTATTTACGACGC TCACCATTAATGGACGCCTGTTTGCTTGCCCGCGAGAGCAATTCGATTCACTGACTCCCTTACCAGAACA GGAAGGCCCAACTGTTGGAACAGTGGGAACTTTTGAACTGATGAGCTCCAAAGATTTAGCATACCAGATG ACAATTTATGATTGGGAACTCTTCAACTGCGTGCATGAGCTGGAGCTAATCTATCACACATTTGGAAGGC ATAATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGATTTAATGAAATTCAGTTTTGGGT CGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGTGTTCAGCTATTAAAAAAATTTATTAAGATA GCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTTTTGCCATCGTCATGGGACTAAGTAACGTTG CTGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCAAGCAAGTTCAAGAAGTTCTATGCGGAGTTTGA AAGTTTAATGGACCCTTCAAGGAACCACAGGGCCTACAGGCTGACAGTAGCTAAGCTGGAACCTCCTCTC ATCCCCTTCATGCCTTTGCTCATTAAAGATATGACATTTACTCATGAGGGGAACAAGACGTTCATTGACA ATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAATACGGCCAGAACAGTGAGATACTACAGGAGCCA ACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACGGCAATTA AATGTGATTGACAACCAGAGAACTTTATCACAGATGTCACACAGATTAGAGCCTCGTCGACCATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001282901
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282901.1, NP_001269830.1
RefSeq Size	3725 bp
RefSeq ORF	2376 bp
Locus ID	11069
UniProt ID	Q8WZA2
Protein Pathways	Leukocyte transendothelial migration
Gene Summary	Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in its 5' UTR, lacks several exons in the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (e) is shorter at the N-terminus, compared to isoform a.

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