EPC1 (NM_001282391) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Epl1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282391, the custom clone sequence may differ by one or more nucleotides ATGGAACATCATCTTCAGCGGGCTATTTCAGCACAGCAGGTGTATGGCGAGAAGAGGGATAATATGGTTA TACCGGTCCCAGAGGCAGAAAGTAATATTGCTTACTATGAGTCTATATATCCTGGGGAATTTAAGATGCC AAAGCAGCTCATTCACATACAGCCTTTTAGTTTGGATGCTGAACAGCCTGATTATGATTTGGATTCTGAA GATGAAGTATTTGTGAATAAACTGAAAAAGAAAATGGACATCTGCCCATTGCAATTTGAGGAGATGATTG ACCGCCTAGAAAAAGGCAGTGGTCAGCAGCCAGTCAGTCTGCAGGAAGCCAAACTACTGCTAAAAGAAGA TGATGAACTAATTAGAGAAGTTTATGAATATTGGATTAAAAAGAGAAAAAACTGTCGAGGGCCATCTCTT ATTCCATCAGTAAAACAAGAGAAGCGAGATGGTTCCAGCACAAATGATCCTTATGTGGCTTTTAGAAGGC GTACTGAAAAAATGCAGACTCGAAAAAATCGCAAAAATGATGAAGCCTCTTACGAAAAAATGCTTAAGCT GCGACGAGATCTAAGTCGAGCTGTTACTATTCTAGAGATGATAAAAAGAAGAGAAAAAAGTAAAAGAGAG CTATTGCACTTAACACTGGAAATTATGGAAAAGAGGTATAATTTGGGCGACTACAATGGAGAGATCATGT CTGAGGTTATGGCACAGAGACAGCCAATGAAACCTACTTATGCCATCCCCATCATCCCTATTACTAATAG CAGTCAATTTAAACACCAGGAAGCAATGGATGTGAAGGAGTTCAAAGTTAATAAGCAAGATAAAGCCGAT CTTATCCGACCGAAACGGAAATATGAAAAGAAGCCCAAAGTCTTACCATCGTCTGCCGCTGCTACTCCCC AACAGACGAGTCCTGCTGCACTGCCAGTCTTCAATGCTAAAGATCTGAATCAGTATGACTTTCCCAGCTC AGACGAAGAACCTCTCTCCCAGGTTTTGTCTGGCTCTTCGGAAGCTGAGGAAGACAATGATCCTGATGGT CCTTTTGCTTTCCGTAGGAAAGCAGGCTGTCAGTACTATGCTCCTCACTTAGACCAAACTGGCAACTGGC CTTGGACTAGTCCTAAAGATGGAGGATTAGGGGATGTGCGATATAGATACTGCTTAACTACTCTCACCGT ACCCCAAAGGTGTATTGGATTTGCACGAAGACGGGTTGGGCGCGGTGGAAGGGTCTTACTGGACAGAGCT CATTCAGACTATGACAGTGTGTTTCACCATCTGGATTTGGAAATGCTTTCCTCACCACAACATTCTCCAG TCAATCAGTTTGCCAATACCTCAGAAACAAATACCTCGGACAAATCTTTCTCTAAAGACCTCAGTCAGAT ACTAGTCAATATCAAATCATGTAGATGGCGGCATTTTAGGCCTCGGACACCATCCCTACATGACAGTGAC AATGATGAACTCTCCTGTAGAAAATTATATAGGAGTATAAACCGAACAGGAACAGCACAACCTGGGACCC AGACATGCAGTACCTCTACGCAAAGTAAAAGTAGCAGTGGTTCAGCACACTTTGCATTTACAGCCGAACA ATACCAGCAACATCAACAGCAACTGGCACTCATGCAGAAACAGCAGCTTGCACAAATTCAGCAACAGCAA GCAAATAGTAATTCCTCCACCAACACATCACAGGGTTTTGTTTCTAAGACTTTGGATTCTGCTAGTGCAC AGTTTGCTGCTTCTGCTTTGGTGACATCAGAACAACTGATGGGATTCAAGATGAAGGATGATGTGGTGCT TGGAATCGGGGTGAATGGCGTCCTTCCAGCCTCAGGAGTATACAAGGGCTTACACCTCAGTAGTACTACA CCAACAGCACTTGTACATACAAGTCCATCAACGGCAGGTTCAGCTTTGTTACAGCCTTCAAATATTACAC AGACTTCAAGTTCCCACAGTGCACTGAGTCATCAAGTAACTGCTGCCAATTCTGCAACAACTCAGGTTCT GATTGGGAACAACATTCGATTAACTGTACCTTCATCAGTTGCCACTGTAAACTCTATTGCCCCAATAAAT GCACGACATATACCTAGGACTTTAAGTGCTGTTCCATCATCTGCCTTAAAGCTGGCCGCTGCAGCAAACT GTCAAGTTTCCAAGGTCCCATCTTCATCCTCTGTAGATTCAGTTCCAAGGGAAAATCATGAATCAGAAAA GCCAGCACTGAACAACATAGCAGACAACACAGTAGCGATGGAGGTGACGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001282391
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282391.1, NP_001269320.1
RefSeq Size	3954 bp
RefSeq ORF	2292 bp
Locus ID	80314
UniProt ID	Q9H2F5
Protein Families	Transcription Factors
Gene Summary	This gene encodes a member of the polycomb group (PcG) family. The encoded protein is a component of the NuA4 histone acetyltransferase complex and can act as both a transcriptional activator and repressor. The encoded protein has been linked to apoptosis, DNA repair, skeletal muscle differentiation, gene silencing, and adult T-cell leukemia/lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (3) represents use of an alternate promoter, and thus differs in the 5' UTR and 5' coding region, and lacks an alternate, in-frame exon in the coding region compared to variant 1. These differences cause translation initiation at an alternate AUG, and the resulting isoform (d) has a distinct N-terminus and is shorter compared to isoform a.

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