ASCC3 (NM_001284271) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASC1p200; HELIC1; RNAH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284271, the custom clone sequence may differ by one or more nucleotides ATGGCTTTACCTCGTCTCACAGGAGCCTTGCGTTCCTTTTCAAATGTCACCAAGCAAGATAATTATAATG AAGAAGTGGCTGACTTAAAGATAAAGCGATCTAAACTTCATGAACAAGTTTTAGATTTGGGCCTGACATG GAAGAAGATAATAAAATTTTTGAATGAAAAACTGGAGAAGAGTAAAATGCAAAGTATAAATGAAGACTTA AAAGATATATTACATGCTGCAAAGCAGATAGTTGGAACTGATAATGGGAGAGAAGCAATTGAAAGTGGGG CTGCATTTCTCTTCATGACATTTCACTTGAAGGACTCTGTTGGTCACAAGGAAACAAAGGCTATCAAACA GATGTTTGGCCCCTTTCCTTCATCATCTGCCACTGCAGCTTGTAATGCTACTAATCGAATTATTTCTCAT TTTAGTCAAGATGATCTTACTGCTCTTGTGCAGATGACAGAAAAAGAACATGGCGATAGGGTTTTTTTTG GTAAAAATTTAGCATTTTCATTTGACATGCATGATTTGGACCACTTTGACGAACTGCCAATAAATGGTGA AACTCAGAAAACTATAAGCCTAGATTATAAGAAGTTTCTGAATGAACATCTCCAGGAGGCTTGCACCCCA GAACTCAAGCCTGTGGAAAAAACAAATGGCTCCTTTTTGTGGTGTGAAGTTGAAAAGTACCTAAATTCAA CTTTGAAGGAAATGACTGAAGTGCCAAGAGTAGAAGATCTTTGCTGTACTTTATATGATATGCTTGCTTC TATTAAAAGTGGTGATGAACTTCAGGATGAGCTATTTGAACTGCTGGGACCTGAAGGACTTGAACTTATT GAGAAACTCCTCCAGAACAGAATTACAATTGTGGATAGATTTCTTAATTCTTCAAATGATCATAGGTTTC AGGCTCTTCAAGACAATTGTAAAAAAATTTTAGGAGAAAATGCTAAACCCAATTATGGTTGTCAAGTCAC TATTCAGTCTGAACAAGAAAAGCAGTTAATGAAACAATATCGACGTGAAGAAAAAAGAATTGCCAGACGA GAAAAAAAGGCTGGAGAAGATTTAGAAGTTTCAGAAGGACTTATGTGCTTTGATCCTAAGGAATTGCGGA TACAAAGAGAACAGGCACTTCTGAATGCTAGAAGTGTTCCAATTCTGAGCAGGCAGAGAGATGCAGACGT TGAAAAAATACATTATCCCCATGTGTATGATTCCCAGGCTGAAGCCATGAAAACATCAGCATTTATTGCT GGTGCAAAGATGATTTTGCCAGAAGGAATCCAAAGAGAGAATAACAAGCTTTATGAAGAAGTAAGGATTC CCTACAGCGAACCAATGCCACTCAGCTTTGAGGAAAAGCCAGTTTATATCCAAGACTTAGATGAGATCGG ACAGCTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATCCAGTCAATAGTGTTTGAGACTGCCTACAAC ACCAATGAGAACATGCTGATTTGTGCCCCTACAGGAGCTGGAAAAACCAACATTGCAATGCTGACAGTCT TGCATGAAATTCGCCAACATTTTCAACAAGGTGTTATCAAAAAGAATGAATTTAAGATTGTATATGTTGC TCCAATGAAAGCCTTGGCAGCTGAAATGACAGATTACTTCAGCAGACGTCTAGAGCCACTAGGCATCATT GTGAAAGAATTGACTGGTGACATGCAGTTGTCCAAAAGTGAAATTTTACGAACTCAGATGCTTGTGACCA CACCAGAAAAATGGGATGTAGTGACAAGAAAGAGTGTTGGGGATGTAGCTCTTTCCCAGATTGTAAGGCT CCTTATTCTTGATGAAGTTCATTTGCTGCATGAAGATAGAGGACCAGTATTAGAAAGCATAGTTGCCCGT ACTTTACGGCAGGTGGAATCCACACAGAGTATGATAAGGATTCTCGGACTGTCTGCAACTTTACCTAACT ACCTCGATGTTGCCACATTTTTACATGTTAATCCATACATTGGACTTTTCTTCTTTGATGGCCGTTTTCG ACCAGTACCTCTTGGACAGACATTTTTGGGGATTAAATGTGCAAATAAGATGCAGCAGTTGAATAACATG GATGAAGTATGTTATGAAAATGTTTTGAAGCAAGTAAAGGCTGGACACCAGGTACACTTATTTTATCTGC TTTTACACTTATTTATCTGCTTTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001284271
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284271.1, NP_001271200.1
RefSeq Size	2849 bp
RefSeq ORF	2196 bp
Locus ID	10973
UniProt ID	Q8N3C0
Protein Families	Transcription Factors
Gene Summary	This gene encodes a protein that belongs to a family of helicases that are involved in the ATP-dependent unwinding of nucleic acid duplexes. The encoded protein is the largest subunit of the activating signal cointegrator 1 complex that is involved in DNA repair and resistance to alkylation damage. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (3) lacks several exons in the 3' coding region and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR compared to variant 1. The encoded protein (isoform c) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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