PIP5KI gamma (PIP5K1C) (NM_001300849) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LCCS3; PIP5K-GAMMA; PIP5K1-gamma; PIP5Kgamma
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300849, the custom clone sequence may differ by one or more nucleotides ATGGAGCTGGAGGTACCGGACGAGGCGGAGAGCGCTGAGGCGGGGGCCGTGCCCTCGGAGGCGGCGTGGG CGGCAGAGAGCGGGGCGGCGGCAGGTTTGGCTCAGAAGAAGGCGGCCCCAACAGAGGTTCTGTCCATGAC GGCACAGCCGGGCCCTGGCCATGGGAAGAAGTTGGGCCATCGAGGTGTGGACGCATCCGGCGAAACCACC TACAAGAAGACCACCTCCTCCACCCTGAAGGGTGCCATCCAGCTGGGCATCGGCTACACCGTGGGCCACC TGAGCTCCAAGCCCGAACGCGACGTGCTCATGCAGGACTTCTACGTGGTGGAGAGCATCTTCTTCCCCAG CGAAGGCAGCAACCTCACCCCCGCCCACCACTTCCAGGACTTCCGCTTCAAGACCTATGCACCTGTCGCC TTCCGCTACTTCCGGGAGCTCTTTGGGATCCGGCCAGATGATTACTTGTACTCCCTGTGCAATGAGCCGC TGATCGAGCTGTCCAACCCGGGCGCCAGTGGCTCCCTCTTCTACGTCACCAGCGACGACGAGTTCATCAT CAAGACCGTCATGCACAAGGAGGCCGAGTTCCTGCAGAAGCTGCTCCCTGGCTACTACATGAACCTCAAC CAGAACCCGCGGACGCTGCTGCCCAAGTTCTATGGGCTGTACTGCGTGCAGTCGGGGGGCAAGAACATCC GCGTCGTGGTCATGAACAACATCCTGCCCCGCGTGGTCAAGATGCACCTCAAGTTCGACCTCAAGGGCTC CACCTACAAGCGGCGCGCCAGCAAGAAGGAGAAGGAGAAGAGCTTCCCCACCTACAAGGACCTGGACTTC ATGCAGGACATGCCCGAGGGGCTCCTGCTGGACGCCGACACCTTCAGCGCCCTGGTCAAGACGCTGCAGC GGGACTGCCTGGTCCTGGAAAGTTTCAAGATCATGGACTACAGCCTGCTGCTGGGCGTGCACAACATCGA CCAGCACGAGCGCGAGCGGCAGGCGCAGGGCGCCCAGAGCACCTCAGATGAGAAGCGGCCTGTGGGCCAG AAGGCGCTCTACTCCACGGCCATGGAGTCCATCCAGGGTGGCGCCGCGCGCGGGGAGGCCATCGAATCGG ATGACACGATGGGCGGGATCCCCGCTGTGAACGGCCGCGGGGAGCGGCTGCTGCTGCACATTGGCATCAT CGACATCCTGCAGTCCTACAGGTTCATCAAGAAACTGGAGCACACCTGGAAGGCCCTCGTCCACGATGGG GACACGGTGTCCGTCCACCGCCCCAGCTTCTATGCCGAGCGCTTTTTCAAGTTCATGAGCAACACGGTCT TTCGGAAGAACTCCTCCCTGAAGTCCTCGCCCTCCAAGAAGGGGCGCGGCGGAGCCTTGCTAGCTGTGAA ACCGCTGGGGCCCACCGCTGCCTTCTCGGCCAGCCAGATCCCTAGCGAGCGGGAGGAGGCCCAGTACGAC CTGCGGGGGGCCCGCAGCTACCCCACGCTGGAGGACGAAGGCCGGCCCGACCTCCTGCCCTGCACGCCAC CTTCTTTCGAAGAAGCCACTACAGCCTCCATTGCCACGACTCTGTCATCCACATCCCTCTCCATTCCTGA GCGGTCCCCCTCGGAGACGTCGGAGCAGCCGCGGTACAGGCGGCGCACACAGTCGTCTGGACAGGATGGC AGGCCGCAGGAGGAGCCACCCGCGGAAGAGGATCTGCAGCAGATTACAGTGCAGGTGGAGCCTGCGTGCA GCGTGGAGATTGTGGTCCCCAAAGAGGAGGACGCAGGGGTGGAGGCTTCCCCGGCCGGTGCCTCTGCTGC TGTTGAAGTAGAAACTGCCAGCCAGGCCTCAGACGAGGAGGGCGCACCTGCCAGCCAGGCCTCGGACGAG GAGGACGCGCCCGCCACCGACATCTACTTTTTCACGGATGGGAGGTACTGGATTTACTCTCCCCGCCATC GCCGACTGCGGGCCGTGACGCTGAGCGCCTCGGGGACTGTAAGTGACCGCAGCCGGCCACCCTGGGGAGA AGGGGCAGTGCCCCTCGGGCAGCAGGGAGCCGCAGGTCCCCGGCCGGAAGCTCAGTGTCTGACGTCAGTT GTTTTCCAGAAGGGCTTTGGGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001300849
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300849.1, NP_001287778.1
RefSeq Size	3035 bp
RefSeq ORF	2124 bp
Locus ID	23396
UniProt ID	O60331
Protein Families	Druggable Genome
Protein Pathways	Endocytosis, Fc gamma R-mediated phagocytosis, Focal adhesion, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
Gene Summary	This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010] Transcript Variant: This variant (3, also known as PIPKIgamma_v5) encodes the longest isoform (3, also known as PIPKIgamma_i5).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.