IRAG1 (NM_001206880) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IRAG; JAW1L; MRVI1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001206880, the custom clone sequence may differ by one or more nucleotides ATGGTAAAAGCTCCCCAGAGTGAAGAGAGGCTGGCCAGAGGAGGAAAGGAGAATAACTCAGTTTTAGCCT GTGGAGCCCAGGCCTCTTGGAGCATCTTTGGGGCTGACGCAGCGGAGGTTCCGGGCACACGTGGCCACTC CCAGCAGGAGGCTGCCATGCCCCACATTCCCGAGGACGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAG AGCCCTGCCGGCCAAGGTCCTCCTGCCGCAGGAGTATCTTGCAGTCCAACTCCCACGATTGTCCTGACTG GGGATGCCACTTCACCAGAAGGAGAAACCGACAAAAACCTGGCCAACAGAGTTCACAGTCCCCACAAGAG GCTTTCTCACCGACACTTGAAGGTGTCCACTGCCTCCCTGACATCTGTGGACCCCGCGGGGCACATCATT GACCTGGTGAATGACCAGCTGCCAGACATCAGCATCTCAGAGGAGGACAAGAAGAAAAACCTGGCGCTGC TGGAAGAAGCCAAGTTGGTGAGTGAGCGATTCCTGACCCGCCGTGGGAGGAAGTCCAGGAGCAGCCCCGG AGACTCCCCATCAGCTGTTTCCCCGAACCTCAGCCCCAGCGCTTCTCCTACATCCTCTCGGAGCAACTCA CTTACAGTCCCCACCCCGCCAGGTTTGGATGTGTGCAGTGGCCCGCCATCCCCTCTGCCTGGAGCACCAC CACAGAAGGGGGATGAGGCCGACGTCTCTTCACCTCACCCTGGCGAGCCTGAGCACATCCTGATGAGAAA TCAGAACTTAGTGGGGCTCAAGCTTCCAGACCTTAGTGAAGCAGCTGAGCAGGAAAAAGGGCTTCCTTCT GAACTCTCCCCAGCTATTGAGGAAGAAGAGTCAAAGAGTGGCTTAGATGTCATGCCTAATATTTCTGATG TGCTGCTGCGCAAACTGCGGGTCCACAGGAGTCTCCCTGGAAGTGCCCCTCCACTCACTGAAAAGGAAGT TGAGAACGTGTTTGTGCAACTGTCCTTGGCCTTTAGAAATGACAGCTACACTCTGGAATCTAGAATTAAC CAGGCTGAAAGGGAACGCAACCTGACAGAGGAGAACACTGAGAAAGAACTGGAAAACTTCAAAGCTTCCA TTACGTCCTCAGCTTCACTCTGGCACCACTGTGAGCACCGGGAAACCTACCAGAAGTTGCTGGAGGACAT CGCTGTCCTGCACCGCCTGGCTGCCCGCCTCTCCAGCCGAGCTGAGGTGGTAGGCGCCGTCCGCCAGGAA AAGCGCATGTCGAAAGCAACGGAAGTGATGATGCAGTATGTGGAGAATCTAAAGAGGACGTATGAGAAGG ACCATGCGGAGCTCATGGAGTTTAAAAAGCTTGCAAATCAGAATTCAAGCCGCAGCTGTGGCCCCTCTGA AGATGGGGTCCCTCGCACGGCACGGTCCATGTCCCTCACGCTGGGAAAGAATATGCCTCGCCGGAGGGTC AGCGTTGCTGTGGTTCCTAAGTTTAATGCCCTGAATCTGCCTGGCCAAACTCCCAGCTCATCATCCATTC CCTCCTTACCAGCCTTGTCGGAATCACCCAATGGGAAAGGCAGCCTACCTGTCACTTCAGCACTGCCTGC ACTTTTGGAAAATGGAAAGACAAATGGGGACCCAGATTGTGAAGCCTCTGCTCCTGCGCTGACCCTGAGC TGCCTGGAGGAGCTTAGTCAGGAGACCAAGGCCAGGATGGAGGAAGAAGCCTACAGCAAGGGATTCCAAG AAGGTCTAAAGAAGACCAAAGAACTTCAAGACCTGAAGGAGGAGGAGGAAGAACAGAAGAGTGAGAGTCC TGAGGAACCTGAAGAGGTAGAAGAAACTGAGGAAGAGGAAAAGGGCCCAAGAAGCAGCAAACTTGAAGAA TTGGTCCATTTCTTACAAGTCATGTATCCCAAACTGTGTCAGCACTGGCAAGTGATCTGGATGATGGCTG CAGTGATGCTGGTCTTGACTGTTGTGCTGGGGCTCTACAATTCCTATAACTCTTGTGCAGAGCAGGCTGA TGGGCCCCTTGGAAGATCCACTTGCTCGGCAGCCCAGAGGGACTCCTGGTGGAGCTCAGGACTCCAGCAT GAGCAGCCTACAGAGCAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001206880
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206880.1, NP_001193809.1
RefSeq Size	5758 bp
RefSeq ORF	2121 bp
Locus ID	10335
UniProt ID	Q9Y6F6
Protein Families	Transmembrane
Protein Pathways	Vascular smooth muscle contraction
Gene Summary	This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011] Transcript Variant: This variant (5) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (e) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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