Nicastrin (NCSTN) (NM_001290184) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATAG1874
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290184, the custom clone sequence may differ by one or more nucleotides ATGGACTTTAATCTCATTTTAGAAAGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATATATATCCCCT TAAATAAAACAGCTCCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATTGGCTGCCAGTCTTCAATTAG TGGAGACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAGGACCTACAGTGGGTATTGACTGATGGCCCC AACCCCCCTTACATGGTTCTGCTGGAGAGCAAGCATTTTACCAGGGATTTAATGGAGAAGCTGAAAGGGA GAACCAGCCGAATTGCTGGTCTTGCAGTGTCCTTGACCAAGCCCAGTCCTGCCTCAGGCTTCTCTCCTAG TGTACAGTGCCCAAATGATGGGTTTGGTGTTTACTCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGA GAAATACAGTGGAATTCGCTGGGCAATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATCTTTCTTCTTG AAGATGAAAATGAAACCAAAGTCATCAAGCAGTGCTATCAAGATCACAACCTGAGTCAGAATGGCTCAGC ACCAACCTTCCCACTATGTGCCATGCAGCTCTTTTCACACATGCATGCTGTCATCAGCACTGCCACCTGC ATGCGGCGCAGCTCCATCCAAAGCACCTTCAGCATCAACCCAGAAATCGTCTGTGACCCCCTGTCTGATT ACAATGTGTGGAGCATGCTAAAGCCTATAAATACAACTGGGACATTAAAGCCTGACGACAGGGTTGTGGT TGCTGCCACCCGGCTGGATAGTCGTTCCTTTTTCTGGAATGTGGCCCCAGGGGCTGAAAGCGCAGTGGCT TCCTTTGTCACCCAGCTGGCTGCTGCTGAAGCTTTGCAAAAGGCACCTGATGTGACCACCCTGCCCCGCA ATGTCATGTTTGTCTTCTTTCAAGGGGAAACTTTTGACTACATTGGCAGCTCGAGGATGGTCTACGATAT GGAGAAGGGCAAGTTTCCCGTGCAGTTAGAGAATGTTGACTCATTTGTGGAGCTGGGACAGGTGGCCTTA AGAACTTCATTAGAGCTTTGGATGCACACAGATCCTGTTTCTCAGAAAAATGAGTCTGTACGGAACCAGG TGGAGGATCTCCTGGCCACATTGGAGAAGAGTGGTGCTGGTGTCCCTGCTGTCATCCTCAGGAGGCCAAA TCAGTCCCAGCCTCTCCCACCATCTTCCCTGCAGCGATTTCTTCGAGCTCGAAACATCTCTGGCGTTGTT CTGGCTGACCACTCTGGTGCCTTCCATAACAAATATTACCAGAGTATTTACGACACTGCTGAGAACATTA ATGTGAGCTATCCCGAATGGCTGAGCCCTGAAGAGGACCTGAACTTTGTAACAGACACTGCCAAGGCCCT GGCAGATGTGGCCACGGTGCTGGGACGTGCTCTGTATGAGCTTGCAGGAGGAACCAACTTCAGCGACACA GTTCAGGCTGATCCCCAAACGGTTACCCGCCTGCTCTATGGGTTCCTGATTAAAGCCAACAACTCATGGT TCCAGTCTATCCTCAGGCAGGACCTAAGGTCCTACTTGGGTGACGGGCCTCTTCAACATTACATCGCTGT CTCCAGCCCCACCAACACCACTTATGTTGTACAGTATGCCTTGGCAAATTTGACTGGCACAGTGGTCAAC CTCACCCGAGAGCAGTGCCAGGATCCAAGTAAAGTCCCAAGTGAAAACAAGGATCTGTATGAGTACTCAT GGGTCCAGGGCCCTTTGCATTCTAATGAGACGGACCGACTCCCCCGGTGTGTGCGTTCTACTGCACGATT AGCCAGGGCCTTGTCTCCTGCCTTTGAACTGAGTCAGTGGAGCTCTACTGAATACTCTACATGGACTGAG AGCCGCTGGAAAGATATCCGTGCCCGGATATTTCTCATCGCCAGCAAAGAGCTTGAGTTGATCACCCTGA CAGTGGGCTTCGGCATCCTCATCTTCTCCCTCATCGTCACCTACTGCATCAATGCCAAAGCTGATGTCCT TTTCATTGCTCCCCGGGAGCCAGGAGCTGTGTCATACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001290184
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290184.1, NP_001277113.1
RefSeq Size	3083 bp
RefSeq ORF	2070 bp
Locus ID	23385
UniProt ID	Q92542
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Alzheimer's disease, Notch signaling pathway
Gene Summary	This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (2) has a different 5' structure resulting in translation initiation at an alternate AUG compared to variant 1; the 5'-most AUG, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the alternate AUG resulting in a shorter isoform (2) with a distinct N-terminus compared to isoform 1.

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