PCDH1 (NM_001278615) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PC42; PCDH42
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278615, the custom clone sequence may differ by one or more nucleotides ATGAATGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCATCAAGATGGGATGGCTA ACATCTCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCTGACCGAGATGAGGGAGA GAATGCAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCCAGGCCAGTGAGACAGGC AGTGACAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGAGAAGGTCAAAGACTACA CCATTGAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAACTCCCTCAAGGTGCAGGT GGTGGACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCGCCTTCCCGGAAAACAAC AAGCCTGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTCTAATGCTGAGCTGGTTT ACTCTCTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAGACTGGAGAGATCCAGGT GAAGACATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGGCAGCTGACCGGGGCAGT CCTAGCCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGACAATGACCCCAAATTTA TGCTGAGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCAGTGGGCATGGTGACTGT CATTGATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGGACAACGGTGACTTTGTT ATCCAGAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCAACAAAGCACCTACACCT TCCAGCTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGTGTCACCATCAATGTGCT GGACGAGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACAAGCTGCTGACCCCCCAG ACACGTCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGGTGTCAATGCTGAGCTGA TCTACAGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACATTCAGGTGCCATCACCCT GGAGAAGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGGTCAGTGACCGCGGCAAG CCCCCACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGCCAACCGCACGCTGCTGG AGACCCTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGGGATCCAGAATATGAGCG CTCCAAGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCGTGGCCTTGCTCATCGCC CTGGCGGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCAGGCTGGTAAGAAGGAGA CCAAGGACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAAAGCAAAGGCAAGAAGAG CAAGTCCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGCAGAAGTCCCTCAAGTTC AACCTGATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAACTACCCACCAGGCAGCC CTGACCTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTGCAGCCCCAGTCACCCTC AGCCTCCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCGTGGGCACCGGGGACACC ACGTCCACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCCCAAATACCCCAGCAAGC AGGTAGGCCAGCCCTTTCAGCTCAGCACACCCCAGCCCCTACCCCACCCCTACCACGGAGCCATCTGGAC CGAGGTGTGGGAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001278615
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278615.1, NP_001265544.1
RefSeq Size	2925 bp
RefSeq ORF	2046 bp
Locus ID	5097
UniProt ID	Q08174
Protein Families	Transcription Factors, Transmembrane
Gene Summary	This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) lacks two 5' and 3' exons, respectively. It has an alternate 5' exon and its transcription extends past a splice site that is used in variant 2, resulting in a novel 3' coding region and 3' UTR compared to variant 2. It encodes isoform 4 which has a shorter N-terminus and a shorter and distinct C-terminus, compared to isoform 2.

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