Estrogen induced gene 121 protein (KIAA1324) (NM_001284353) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIG121; KIAA1324
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284353, the custom clone sequence may differ by one or more nucleotides ATGTTTCATTCATCTCTGAGACACCAGACACAACTCATGTACAAATGGGCCAAGCCGAAAATCTGTAGCG AGGACCTTGAGGGGGCAGTGAAGCTGCCTGCCTCTGGTGTGAAGACCCACTGCCCACCCTGCAACCCAGG CTTCTTCAAAACCAACAACAGCACCTGCCAGCCCTGCCCATATGGTTCCTACTCCAATGGCTCAGACTGT ACCCGCTGCCCTGCAGGGACTGAACCTGCTGTGGGATTTGAATACAAATGGTGGAACACGCTGCCCACAA ACATGGAAACGACCGTTCTCAGTGGGATCAACTTCGAGTACAAGGGCATGACAGGCTGGGAGGTGGCTGG TGATCACATTTACACAGCTGCTGGAGCCTCAGACAATGACTTCATGATTCTCACTCTGGTTGTGCCAGGA TTTAGACCTCCGCAGTCGGTGATGGCAGACACAGAGAATAAAGAGGTGGCCAGAATCACATTTGTCTTTG AGACCCTCTGTTCTGTGAACTGTGAGCTCTACTTCATGGTGGGTGTGAATTCTAGGACCAACACTCCTGT GGAGACGTGGAAAGGTTCCAAAGGCAAACAGTCCTATACCTACATCATTGAGGAGAACACTACCACGAGC TTCACCTGGGCCTTCCAGAGGACCACTTTTCATGAGGCAAGCAGGAAGTACACCAATGACGTTGCCAAGA TCTACTCCATCAATGTCACCAATGTTATGAATGGTGTGGCCTCCTACTGCCGTCCCTGTGCCCTAGAAGC CTCTGATGTGGGCTCCTCCTGCACCTCTTGTCCTGCTGGTTACTATATTGACCGAGATTCAGGAACCTGC CACTCCTGCCCCACTAACACAATTCTGAAAGCCCACCAGCCTTATGGTGTCCAGGCCTGTGTGCCCTGTG GTCCAGGGACCAAGAACAACAAGATCCACTCTCTGTGCTACAACGATTGCACCTTCTCACGCAACACTCC GACCAGGACTTTCAACTACAACTTCTCCGCTTTGGCAAACACTGTCACTCTTGCTGGAGGGCCAAGCTTC ACTTCCAAAGGGCTGAAATACTTCCATCACTTTACCCTCAGTCTCTGTGGAAACCAGGGTAGGAAAATGT CTGTGTGCACCGACAATGTCACTGACCTCCGGATTCCTGAGGGTGAGTCAGGGTTCTCCAAATCTATCAC AGCCTACGTCTGCCAGGCAGTCATCATCCCCCCAGAGGTGACAGGCTACAAGGCCGGGGTTTCCTCACAG CCTGTCAGCCTTGCTGATCGACTTATTGGGGTGACAACAGATATGACTCTGGATGGAATCACCTCCCCAG CTGAACTTTTCCACCTGGAGTCCTTGGGAATACCGGACGTGATCTTCTTTTATAGGTCCAATGATGTGAC CCAGTCCTGCAGTTCTGGGAGATCAACCACCATCCGCGTCAGGTGCAGTCCACAGAAAACTGTCCCTGGA AGTTTGCTGCTGCCAGGAACGTGCTCGGATGGGACCTGTGATGGCTGCAACTTCCACTTCCTGTGGGAGA GCGCGGCTGCTTGCCCGCTCTGCTCAGTGGCTGACTACCATGCTATCGTCAGCAGCTGTGTGGCTGGGAT CCAGAAGACTACTTACGTGTGGCGAGAACCCAAGCTATGCTCTGGTGGCATTTCTCTGCCTGAGCAGAGA GTCACCATCTGCAAAACCATAGATTTCTGGCTGAAAGTGGGCATCTCTGCAGGCACCTGTACTGCCATCC TGCTCACCGTCTTGACCTGCTACTTTTGGAAAAAGAATCAAAAACTAGAGTACAAGTACTCCAAGCTGGT GATGAATGCTACTCTCAAGGACTGTGACCTGCCAGCAGCTGACAGCTGCGCCATCATGGAAGGCGAGGAT GTAGAGGACGACCTCATCTTTACCAGCAAGAAGTCACTCTTTGGGAAGATCAAATCATTTACCTCCAAGA GGACTCCTGATGGATTTGACTCAGTGCCGCTGAAGACATCCTCAGGAGGCCTAGACATGGACCTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001284353
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284353.1, NP_001271282.1
RefSeq Size	6914 bp
RefSeq ORF	2028 bp
Locus ID	57535
Protein Families	Transmembrane
Gene Summary	Expression of this gene is induced by estrogen and the encoded protein has been characterized as a transmembrane protein. The encoded protein has been found in to correlate with survival in certain carcinomas (PMID: 21102415) and may be important for cellular response to stress (PMID: 21072319). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012] Transcript Variant: This variant (5) represents use of an alternate promoter, and thus differs in the 5' UTR and 5' coding region. These differences cause translation initiation at an alternate start codon and result in an isoform (5) with a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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