HJURP (NM_001282963) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FAKTS; hFLEG1; URLC9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337147 representing NM_001282963. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGGGTACGCTGCGCGCCATGGAGGGCGAGGACGTGGAAGACGACCAGCTGCTGCAGAAGCTCAGG GCCAGTCGCCGCCGCTTCCAGAGGCGCATGCAGCGGCTGATAGAGAAGTACAACCAGCCCTTCGAGGAC ACCCCGGTGGTGCAAATGGCCACGCTGACCTACGAGACGCCACAGGGATTGAGAATTTGGGGTGGAAGA CTAATAAAGGAAAGAAACGAAGGAGAGATCCAGTGTGCAGGTAACAGAGCTGGAAGGGATGTACGTGTG ACTCCGCTGCCTTCACTGGCCTCACCTGCCGTGCCTGCCCCCGGATACTGCAGTCGTATCTCCAGAAAG AGTCCTGGTGACCCAGCGAAACCAGCTTCATCTCCCAGAGAATGGGATCCTTTGCATCCTTCCTCCACA GACATGGCCTTAGTACCTAGAAATGACAGCCTCTCCCTACAAGAGACCAGTAGCAGCAGCTTCTTAAGC AGCCAGCCCTTTGAAGATGATGACATTTGCAATGTGACCATCAGTGACCTGTACGCAGGGATGCTGCAC TCCATGAGCCGGCTGTTGAGCACAAAGCCATCAAGCATCATCTCCACCAAAACGTTCATCATGCAAAAC TGGAACTCCAGGAGGAGGCACAGATATAAGAGCAGGATGAACAAAACATATTGCAAAGGAGCCAGACGT TCTCAGAGGAGCTCCAAGGAGAACTTCATACCCTGCTCTGAGCCTGTGAAAGGGACAGGGGCATTAAGA GATTGCAAGAACGTATTAGATGTTTCTTGCCGTAAGACAGGTTTAAAATTGGAAAAAGCTTTTCTTGAA GTCAACAGACCCCAAATCCATAAGTTAGATCCAAGTTGGAAGGAGCGCAAAGTGACACCCTCGAAGTAT TCTTCCTTGATTTACTTCGACTCCAGTGCAACATATAATCTTGATGAGGAAAATAGATTTAGGACATTA AAATGGTTAATTTCTCCTGTAAAAATAGTTTCCAGACCAACAATACGACAGGGCCATGGAGAGAACCGT CAGAGGGAGATTGAAATCCGATTTGATCAGCTTCATCGGGAATATTGCCTGAGTCCCAGGAACCAGCCT CGCCGGATGTGCCTCCCGGACTCCTGGGCCATGAACATGTACAGAGGGGGTCCTGCGAGTCCTGGTGGC CTTCAGGGCTTAGAAACCCGCAGGCTGAGTTTACCTTCCAGCAAAGCAAAAGCAAAAAGTTTAAGTGAG GCTTTTGAAAACCTAGGCAAAAGATCTCTGGAAGCAGGTAGGTGCCTGCCCAAGAGCGATTCATCTTCA TCACTTCCAAAGACCAACCCCACACACAGCGCAACTCGCCCGCAGCAGACATCTGACCTTCACGTTCAG GGAAATAGTTCTGGAATATTTAGAAAGTCAGTGTCACCCAGCAAAACTCTTTCAGTCCCAGATAAAGAA GTGCCAGGCCACGGAAGGAATCGTTACGATGAAATTAAAGAAGAATTTGACAAGCTTCATCAAAAGTAT TGCCTCAAATCTCCTGGGCAGATGACAGTGCCTTTATGTATTGGAGTGTCTACAGATAAAGCAAGTATG GAAGTTCGATATCAAACAGAAGGCTTCTTAGGAAAATTAAATCCAGACCCTCACTTCCAGGGTTTCCAG AAGTTGCCATCATCACCCCTGGGGTGCAGAAAAAGTCTACTGGGCTCAACTGCAATTGAGGCTCCTTCA TCTACATGTGTTGCTCGTGCCATCACGAGGGATGGCACGAGGGACCATCAGTTCCCTGCAAAAAGACCC AGGCTATCAGAACCCCAGGGCTCCGGACGCCAGGGCAATTCCCTGGGTGCCTCAGATGGGGTGGACAAC ACCGTCAGACCGGGAGACCAGGGCAGCTCTTCACAGCCCAACTCAGAAGAGAGAGGAGAGAACACGTCT TACAGGATGGAAGAGAAAAGTGATTTCATGCTAGAAAAATTGGAAACTAAAAGTGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282963
Insert Size	1992 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282963.1
RefSeq Size	2934 bp
RefSeq ORF	1992 bp
Locus ID	55355
UniProt ID	Q8NCD3
Protein Families	Druggable Genome
MW	74.4 kDa
Gene Summary	Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks three alternate exons that result in the loss of an in-frame segment in the 5' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a.

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