HGFAC (NM_001297439) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HGFA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337144 representing NM_001297439. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGCGCTGGGCCTGGGTCCCCAGCCCCTGGCCCCCACCGGGGCTGGGCCCCTTCCTCCTCCTCCTC CTGCTGCTGCTGCTGCTGCCACGGGGGTTCCAGCCCCAGCCTGGCGGGAACCGTACGGAGTCCCCAGAA CCTAATGCCACAGCGACCCCTGCGATCCCCACTATCCTGGTGACCTCTGTGACCTCTGAGACCCCAGCA ACAAGTGCTCCAGAGGCAGAGGGACCCCAAAGTGGGGGGCTCCCGCCCCCGCCCAGGGCAGTTCCCTCG AGCAGTAGCCCCCAGGCCCAAGCACTCACCGAGGACGGGAGGCCCTGCAGGTTCCCCTTCCGCTACGGG GGCCGCATGCTGCATGCCTGCACTTCGGAGGGCAGTGCACACAGGAAGTGGTGTGCCACAACTCACAAC TACGACCGGGACAGGGCCTGGGGCTACTGTGTGGAGGCCACCCCGCCTCCAGGGGGCCCAGCTGCCCTG GATCCCTGTGCCTCCGGCCCCTGCCTCAATGGAGGCTCCTGCTCCAATACCCAGGACCCCCAGTCCTAT CACTGCAGCTGCCCCCGGGCCTTCACCGGCAAGGACTGCGGCACAGAGAAATGCTTTGATGAGACCCGC TACGAGTACCTGGAGGGGGGCGACCGCTGGGCCCGCGTGCGCCAGGGCCACGTGGAACAGTGCGAGTGC TTCGGGGGCCGGACCTGGTGCGAAGGCACCCGACATACAGCTTGTCTGAGCAGCCCTTGCCTGAACGGG GGCACCTGCCACCTGATCGTGGCCACCGGGACCACCGTGTGTGCCTGCCCACCAGGCTTCGCTGGACGG CTCTGCAACATCGAGCCTGATGAGCGCTGCTTCTTGGGGAACGGCACTGGGTACCGTGGCGTGGCCAGC ACCTCAGCCTCGGGCCTCAGCTGCCTGGCCTGGAACTCCGATCTGCTCTACCAGGAGCTGCACGTGGAC TCCGTGGGCGCCGCGGCCCTGCTGGGCCTGGGCCCCCATGCCTACTGCCGGAATCCGGACAATGACGAG AGGCCCTGGTGCTACGTGGTGAAGGACAGCGCGCTCTCCTGGGAGTACTGCCGCCTGGAGGCCTGCGAT TTGGAAACTGAGGGCAGAGAATCCCTCACCAGAGTCCAACTGTCACCGGATCTCCTGGCGACCCTGCCT GAGCCAGCCTCCCCGGGGCGCCAGGCCTGCGGCAGGAGGCACAAGAAGAGGACGTTCCTGCGGCCACGT ATCATCGGCGGCTCCTCCTCGCTGCCCGGCTCGCACCCCTGGCTGGCCGCCATCTACATCGGGGACAGC TTCTGCGCCGGGAGCCTGGTCCACACCTGCTGGGTGGTGTCGGCCGCCCACTGCTTCTCCCACAGCCCC CCCAGGGACAGCGTCTCCGTGGTGCTGGGCCAGCACTTCTTCAACCGCACGACGGACGTGACGCAGACC TTCGGCATCGAGAAGTACATCCCGTACACCCTGTACTCGGTGTTCAACCCCAGCGACCACGACCTCGTC CTGATCCGGCTGAAGAAGAAAGGGGACCGCTGTGCCACACGCTCGCAGTTCGTGCAGCCCATCTGCCTG CCCGAGCCCGGCAGCACCTTCCCCGCAGGACACAAGTGCCAGATTGCGGGCTGGGGCCACTTGGATGAG AACGTGAGCGGCTACTCCAGCTCCCTGCGGGAGGCCCTGGTCCCCCTGGTCGCCGACCACAAGTGCAGC AGCCCTGAGGTCTACGGCGCCGACATCAGCCCCAACATGCTCTGTGCCGGCTACTTCGACTGCAAGTCC GACGCCTGCCAGGGGGACTCAGGGGGGCCCCTGGCCTGCGAGAAGAACGGCGTGGCTTACCTCTACGGC ATCATCAGCTGGGGTGACGGCTGCGGGCGGCTCCACAAGCCGGGGGTCTACACCCGCGTGGCCAACTAT GTGGACTGGATCAACGACCGGATACGGCCTCCCAGGCGGCTTGTGGCTCCCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297439
Insert Size	1989 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297439.1
RefSeq Size	2134 bp
RefSeq ORF	1989 bp
Locus ID	3083
UniProt ID	Q04756
Protein Families	Druggable Genome, Protease, Secreted Protein, Transmembrane
MW	71.5 kDa
Gene Summary	This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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