IL12RB1 (NM_001290023) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD212; IL-12R-BETA1; IL12RB; IMD30
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337139 representing NM_001290023. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCCGCTGGTGACCTGGGTGGTCCCCCTCCTCTTCCTCTTCCTGCTGTCCAGGCAGGGCGCTGCC TGCAGAACCAGTGAGTGCTGTTTTCAGGACCCGCCATATCCGGATGCAGACTCAGGCTCGGCCTCGGGC CCTAGGGACCTGAGATGCTATCGGATATCCAGTGATCGTTACGAGTGCTCCTGGCAGTATGAGGGTCCC ACAGCTGGGGTCAGCCACTTCCTGCGGTGTTGCCTTAGCTCCGGGCGCTGCTGCTACTTCGCCGCCGGC TCAGCCACCAGGCTGCAGTTCTCCGACCAGGCTGGGGTGTCTGTGCTGTACACTGTCACACTCTGGGTG GAATCCTGGGCCAGGAACCAGACAGAGAAGTCTCCTGAGGTGACCCTGCAGCTCTACAACTCAGTTAAA TATGAGCCTCCTCTGGGAGACATCAAGGTGTCCAAGTTGGCCGGGCAGCTGCGTATGGAGTGGGAGACC CCGGATAACCAGGTTGGTGCTGAGGTGCAGTTCCGGCACCGGACACCCAGCAGCCCATGGAAGTTGGGC GACTGCGGACCTCAGGATGATGATACTGAGTCCTGCCTCTGCCCCCTGGAGATGAATGTGGCCCAGGAA TTCCAGCTCCGACGACGGCAGCTGGGGAGCCAAGGAAGTTCCTGGAGCAAGTGGAGCAGCCCCGTGTGC GTTCCCCCTGAAAACCCCCCACAGCCTCAGGTGAGATTCTCGGTGGAGCAGCTGGGCCAGGATGGGAGG AGGCGGCTGACCCTGAAAGAGCAGCCAACCCAGCTGGAGCTTCCAGAAGGCTGTCAAGGGCTGGCGCCT GGCACGGAGGTCACTTACCGACTACAGCTCCACATGCTGTCCTGCCCGTGTAAGGCCAAGGCCACCAGG ACCCTGCACCTGGGGAAGATGCCCTATCTCTCGGGTGCTGCCTACAACGTGGCTGTCATCTCCTCGAAC CAATTTGGTCCTGGCCTGAACCAGACGTGGCACATTCCTGCCGACACCCACACAGAACCAGTGGCTCTG AATATCAGCGTCGGAACCAACGGGACCACCATGTATTGGCCAGCCCGGGCTCAGAGCATGACGTATTGC ATTGAATGGCAGCCTGTGGGCCAGGACGGGGGCCTTGCCACCTGCAGCCTGACTGCGCCGCAAGACCCG GATCCGGCTGGAATGGCAACCTACAGCTGGAGTCGAGAGTCTGGGGCAATGGGGCAGGAAAAGTGTTAC TACATTACCATCTTTGCCTCTGCGCACCCCGAGAAGCTCACCTTGTGGTCTACGGTCCTGTCCACCTAC CACTTTGGGGGCAATGCCTCAGCAGCTGGGACACCGCACCACGTCTCGGTGAAGAATCATAGCTTGGAC TCTGTGTCTGTGGACTGGGCACCATCCCTGCTGAGCACCTGTCCCGGCGTCCTAAAGGAGTATGTTGTC CGCTGCCGAGATGAAGACAGCAAACAGGTGTCAGAGCATCCCGTGCAGCCCACAGAGACCCAAGTTACC CTCAGTGGCCTGCGGGCTGGTGTAGCCTACACGGTGCAGGTGCGAGCAGACACAGCGTGGCTGAGGGGT GTCTGGAGCCAGCCCCAGCGCTTCAGCATCGAAGTGCAGGTTTCTGATTGGCTCATCTTCTTCGCCTCC CTGGGGAGCTTCCTGAGCATCCTTCTCGTGGGCGTCCTTGGCTACCTTGGCCTGAACAGGGCCGCACGG CACCTGTGCCCGCCGCTGCCCACACCCTGTGCCAGCTCCGCCATTGAGTTCCCTGGAGGGAAGGAGACT TGGCAGTGGATCAACCCAGTGGACTTCCAGGAAGAGGCATCCCTGCAGGAGGCCCTGGTGGTAGAGATG TCCTGGGACAAAGGCGAGAGGACTGAGCCTCTCGAGAAGACAGAGCTACCTGAGGGTGCCCCTGAGCTG GCCCTGGATACAGAGTTGTCCTTGGAGGATGGAGACAGATGTGATCGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290023
Insert Size	1983 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290023.1
RefSeq Size	2787 bp
RefSeq ORF	1983 bp
Locus ID	3594
UniProt ID	P42701
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
MW	72.9 kDa
Gene Summary	The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (3) represents the use of an alternate promoter and has multiple differences compared to variant 4, one of which results in a translational frameshift. These differences result in distinct 5' and 3' UTRs and the use of a downstream start codon, causing the encoded isoform (3, also known as Short) to contain a shorter N-terminus and a distinct C-terminus compared to isoform 4. Sequence Note: The 5' UTR was inferred from partial sequence data.

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