CUL4A (NM_001278513) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC337134 representing NM_001278513. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTCTACAAGCAACTGCGTCAGGCCTGTGAAGACCACGTCCAGGCACAGATCCTTCCGTTTAGAGAA GACTCACTAGATAGTGTTTTATTTTTAAAGAAGATTAACACGTGCTGGCAGGACCACTGCAGACAAATG ATCATGATCAGAAGCATCTTCCTGTTCTTGGACCGCACCTATGTGCTGCAGAACTCCACGCTGCCCTCC ATCTGGGATATGGGATTAGAACTGTTTAGAACCCATATTATTAGTGATAAAATGGTTCAGAGTAAAACC ATTGATGGAATCCTACTGCTGATCGAGCGCGAGAGGAGCGGCGAGGCCGTGGACCGGAGCCTGTTGCGG AGCCTCCTGGGCATGCTGTCTGACCTGCAGGTGTATAAAGATTCATTTGAACTGAAATTTTTGGAAGAG ACTAATTGCTTATATGCTGCCGAAGGCCAAAGGTTAATGCAGGAAAGAGAGGTTCCAGAATATCTTAAC CATGTAAGTAAACGCTTAGAGGAAGAGGGAGACAGAGTAATCACTTACTTGGACCACAGCACACAGAAA CCACTGATTGCTTGTGTGGAGAAACAGCTATTAGGAGAACATTTAACAGCAATTCTGCAGAAAGGGCTC GACCACTTACTGGATGAGAACAGAGTGCCGGACCTCGCACAGATGTACCAGCTGTTCAGCCGGGTGAGG GGCGGGCAGCAGGCGCTGCTGCAGCACTGGAGCGAGTACATCAAGACTTTTGGAACAGCGATCGTAATC AATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGTTGGACTTCAAGGACAAGGTGGACCACGTGATC GAGGTCTGCTTCCAGAAGAATGAGCGGTTCGTCAACCTGATGAAGGAGTCCTTTGAGACGTTCATCAAC AAGAGACCCAACAAGCCTGCAGAACTGATCGCAAAGCATGTGGATTCAAAGTTAAGAGCAGGCAACAAA GAAGCCACAGACGAGGAGCTGGAGCGGACGTTGGACAAGATCATGATCCTGTTCAGGTTTATCCACGGT AAAGATGTCTTTGAAGCATTTTATAAAAAAGATTTGGCAAAAAGACTCCTTGTTGGGAAAAGTGCCTCA GTCGATGCTGAAAAGTCTATGTTGTCAAAGCTCAAGCATGAGTGCGGTGCAGCCTTCACCAGCAAGCTG GAAGGCATGTTCAAGGACATGGAGCTTTCGAAGGACATCATGGTTCATTTCAAGCAGCATATGCAGAAT CAGAGTGACTCAGGCCCTATAGACCTCACAGTGAACATACTCACAATGGGCTACTGGCCAACATACACG CCCATGGAAGTGCACTTAACCCCAGAAATGATTAAACTTCAGGAAGTATTTAAGGCATTTTATCTTGGA AAGCACAGTGGTCGAAAACTTCAGTGGCAAACTACTTTGGGACATGCTGTTTTAAAAGCGGAGTTTAAA GAAGGGAAGAAGGAATTCCAGGTGTCCCTCTTCCAGACACTGGTGCTCCTCATGTTCAACGAGGGAGAT GGCTTCAGCTTTGAGGAGATAAAAATGGCCACGGGGATAGAGGATAGTGAATTGCGCAGAACGCTGCAG TCCCTGGCCTGTGGCAAAGCACGTGTGCTGATTAAAAGTCCCAAAGGAAAGGAAGTGGAAGATGGAGAC AAGTTCATTTTTAATGGAGAGTTCAAGCACAAGTTGTTTAGAATAAAGATCAATCAAATTCAGATGAAG GAAACTGTTGAGGAACAGGTTAGCACCACTGAGAGAGTGTTTCAGGATAGACAATATCAGATTGATGCT GCTATCGTCAGAATAATGAAGATGAGAAAGACTCTTGGTCATAATCTTCTAGTTTCTGAATTATATAAT CAGCTGAAATTTCCAGTAAAGCCTGGAGATTTGAAAAAGAGAATTGAATCTCTGATAGACAGAGACTAT ATGGAGAGAGACAAAGACAATCCGAATCAGTACCACTACGTGGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001278513
Insert Size	1980 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278513.1
RefSeq Size	4053 bp
RefSeq ORF	1980 bp
Locus ID	8451
UniProt ID	Q13619
Protein Pathways	Nucleotide excision repair, Ubiquitin mediated proteolysis
MW	76.8 kDa
Gene Summary	CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009] Transcript Variant: This variant (3) utilizes an alternate 5' terminal exon, compared to variant 1, resulting in an isoform (2) with a shorter N-terminus. Variants 2, 3, 5, 6, and 7 all encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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