PCDHB6 (NM_001303145) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PCDH-BETA6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337129 representing NM_001303145. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTCCTGAAAATATCAGAAATTACTATGCCAGGAAAGATATTTCCTTTGAAAATGGCACACGATTTA GACACCGGCAGCAACGGCCTTCAGAGGTACACAATCAGCTCCAACCCTCACTTCCACGTTCTCACCCGC AATCGCAGCGAAGGCAGGAAGTTCCCGGAGCTGGTGCTAGACAAACCGTTGGACCGCGAGGAGCAGCCC CAACTCAGGCTAACGCTGATCGCGCTGGATGGCGGGTCTCCGCCCCGGTCAGGGACCTCCGAGATTCAG ATCCAGGTTTTGGACATCAATGACAACGTCCCCGAGTTTGCTCAGGAGCTCTATGAAGCACAAGTCCCT GAGAACAACCCCCTCGGCTCTCTGGTTATTACCGTCTCAGCCAGAGATTTAGATGCAGGATCGTTTGGG AAGGTATCTTACGCCCTGTTTCAAGTCGATGACGTCAACCAACCCTTCGAAATAAACGCAATCACAGGA GAAATTCGGCTGAGAAAGGCTTTGGATTTTGAGGAAATTCAGTCTTATGACGTGGATGTTGAGGCTACA GATGGTGGAGGCCTATCAGGAAAATGCTCTTTAGTCGTCAGGGTCCTGGACGTGAATGACAATGCCCCT GAACTCACCATGTCGTTCTTCATCAGCCTCATCCCAGAAAACTTACCAGAGATCACAGTGGCAGTTTTC AGTGTTTCAGATGCAGACTCTGGACATAACCAACAGGTTATTTGTTCAATAGAGAACAATCTCCCCTTT CTACTAAGACCTTCCGTGGAGAATTTCTACACCCTGGTAACAGAAGGCGCGCTGGACAGAGAGAGCAGA GCCGAGTACAACATCACTATCACGGTCACTGATTTGGGGACACCAAGGCTGAAAACCCAGCAGAGCATA ACTGTGCAGGTCTCCGACGTCAATGACAACGCCCCCGCCTTCACCCAAACCTCCTACACCCTGTTCGTC CGCGAGAACAACAGCCCCGCCCTGCACATCGGCAGCGTCAGCGCCACAGACAGAGACTCAGGCATCAAC GCCCAGGTCACCTACTCGCTGCTGCCGCCCCAGGACCCGCACCTGCCCCTCTCTTCCCTGGTCTCCATC AACGCGGACAACGGCCACCTGTTTGCCCTCAGGTCGCTGGACTACGAGGCCCTGCAGTCTTTCGAGTTC CGCGTGGGCGCCACAGACCGCGGCTCCCCGGCGTTGAGCAGCGAGGCGCTGGTGCGCTTGCTGGTGCTG GACGCCAACGACAACTCGCCCTTCGTGTTGTACCCGCTGCAGAACGGCTCCGCGCCCTGCACCGAGCTG GTGCCCCGGGCGGCCGAGCCGGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAG AACGCCTGGCTGTCGTACCAGCTGCTCAAGGCCACGGAGCTCGGTCTGTTCGGCGTGTGGGCGCACAAT GGCGAGGTGCGCACCGCCAGGCTGCTGAGCGAGCGAGACGCAGCCAAGCACAGGCTGGTGGTGCTTGTC AAGGACAATGGCGAGCCTCCGCGCTCGGCCACCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCC CAGCCCTACCTGCCTCTCCCTGAGGCGGCCCCGGCCCAAGCCCAGGCCGACTCTCTCACCGTCTACCTG GTGGTGGCGTTGGCCTCGGTGTCGTCGCTCTTCCTCTTTTCGGTGCTCCTGTTCGTGGCGGTGCGGCTG TGCAGGAGGAGCAGGGCGGCCTCGGTGGGTCGCTACTCGGTGCCCGAGGGTCCCTTTCCAGGGCATCTG GTGGATGTGAGCGGCACCGGGACCCTATCCCAGAGCTACCAGTACAAGGTGTGTCTGACGGGAGGCTCA GAAACAAATGAGTTCAAGTTCCTGAAGCCGATTATGCCCAACTTCCCTCCTCAGGGCACTGAGAGAGAA ATGGAAGAAACCCCCACCTCTCGGAATAGCTTCCCGTTCAGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303145
Insert Size	1977 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303145.1
RefSeq Size	3061 bp
RefSeq ORF	1977 bp
Locus ID	56130
UniProt ID	Q9Y5E3
Protein Families	Transmembrane
MW	71.8 kDa
Gene Summary	This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. Unlike the alpha and gamma clusters, the transcripts from these genes do not share common 3' exons. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell neural connections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) lacks a portion of the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.

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