ANKLE1 (NM_001278444) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANKRD41; LEM3; LEMD6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337102 representing NM_001278444. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGACCCCCGTGCGGCGCGGGGCAGCCTGGCTGGGAACCCTGCTCGGCCCTGGAGAGGGGCCCCCC GAGCCGAGGGGAGGAGCGGCGCATGCCCACAAGCCCCCCGCGGGAAGTAGGAAATCGACCGACCAGGCG GTGCGCTTCGGACCCAGCCAGGGCATGTGCTCGGAGGCCCGCCTGGCTCGCAGGTTGCGGGATGCGCTG CGGGAGGAGGAGCCGTGGGCAGTAGAGGAGCTGCTGCGCTGCGGCGCGGACCCTAATTTGGTGCTAGAG GACGGCGCAGCGGCTGTGCACTTGGCGGCCGGAGCCCGGCACCCGCGCGGCCTGCGTTGCCTCGGGGCC CTACTGCGCCAAGGCGGGGACCCCAACGCTCGATCTGTCGAGGCACTGACGCCGCTGCATGTGGCCGCC GCGTGGGGCTGCCGCCGCGGCCTGGAGCTGCTGCTGAGCCAAGGAGCGGACCCGGCGCTGCGCGACCAG GACGGACTCCGGCCGCTGGACCTGGCCCTGCAGCAGGGACACCTGGAGTGCGCGCGAGTCCTGCAGGAT CTCGACACGCGGACCAGGACCCGGACCCGGATCGGGGCAGAGACTCAGGAGCCCGAGCCTGCACCTGGC ACCCCAGGCCTCTCTGGACCTACCGATGAGACGCTGGACTCCATAGCACTCCAAAAGCAGCCATGCAGA GGTGACAACAGGGACATTGGCTTGGAGGCTGACCCAGGACCCCCCAGCCTCCCTGTTCCCCTTGAAACT GTGGACAAACATGGGAGCTCGGCGTCCCCTCCAGGGCACTGGGATTACAGCTCAGACGCCTCTTTCGTC ACAGCGGTTGAGGTCTCTGGAGCTGAGGACCCAGCCTCGGACACTCCCCCCTGGGCTGGGTCATTGCCA CCGACCAGGCAGGGACTTCTGCATGTTGTCCATGCCAACCAGAGGGTACCTAGGTCTCAGGGCACGGAG GCAGAACTGAATGCCCGTCTGCAGGCCCTGACTCTGACCCCACCAAATGCTGCTGGCTTCCAGTCCTCC CCTTCCTCCATGCCTCTCCTGGACAGGAGTCCAGCTCATAGCCCCCCACGGACACCAACCCCTGGAGCT TCTGACTGCCACTGCCTGTGGGAGCACCAGACATCCATTGATAGTGACATGGCCACGCTCTGGCTGACA GAGGATGAGGCAAGCTCTACAGGTGGCAGGGAACCTGTCGGCCCTTGCCGGCACCTGCCAGTCTCCACT GTGTCTGACTTGGAGTTGCTGAAGGGACTCCGAGCACTTGGCCCAGAGTTTTCAGGGCACAGCCTAGAA CTGGCTGCAGCCCTGCGGACGGGCTGTATTCCAGATGTCCAGGCAGATGAAGACGCGCTGGCCCAGCAG TTTGAGCAGCCAGATCCTGCCAGGAGGTGGCGGGAGGGGGTCGTGAAGTCTAGCTTCACCTATCTGCTG CTGGACCCCAGGGAGACTCAGGACCTGCCAGCCCGAGCCTTCTCACTGACCCCAGCTGAGCGCCTTCAG ACTTTCATCCGTGCCATCTTCTACGTGGGCAAAGGGACGAGGGCCCGGCCATATGTCCACCTCTGGGAG GCCCTTGGTCACCATGGGCGGTCAAGAAAACAGGGATCCAGACGCTCACCAACCAGAAGCAAGGGCACT GCTATGGAGTGGTGGCAGGCTGGCCACCTGCTCGTCGCCGGCGCTTGGGGGTGCACCTGCTGCACCGTG CCCTCCTTGTCTTCCTGGCTGAAGGCGAGCGACAGCTTCATCCCCAGGACATCCAGGCCCGGGGCTGAG TGCTGGGGAGTTGGCCATCCAGCCTGGGGAGAAATGTTTGAATGTTCCAGCTGCCCCATGCTGACAGCA GCCCCCATCTCTGGTTTCAGAAGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT TTGTGTGTGTGTGTGTGTGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278444
Insert Size	1956 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278444.1
RefSeq Size	3001 bp
RefSeq ORF	1956 bp
Locus ID	126549
MW	69.8 kDa
Gene Summary	Endonuclease that probably plays a role in the DNA damage response and DNA repair.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region and lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and contains a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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