PIB5PA (INPP5J) (NM_001284289) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	INPP5; PIB5PA; PIPP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337074 representing NM_001284289. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCTCCCAAGGCTTGGCACACAGAGTACAGGGCCTGGCAGGTGCCTGAGCCCCAACCTTCAGGCC CAAGAAGCCCCAGCCCCAGTCACCACCTCCTCTTCTACATCCACCCTGTCATCCTCCCCTTGGTCAGCT CAGCCTACCTGGAAGAGCGACCCCGGCTTCCGGATCACTGTGGTCACATGGAACGTGGGCACTGCCATG CCCCCAGACGATGTCACATCCCTCCTCCACCTGGGCGGTGGTGACGACAGCGACGGCGCAGACATGATC GCCATAGGGTTGCAGGAAGTGAACTCCATGCTCAACAAGCGACTCAAGGACGCCCTCTTCACGGACCAG TGGAGTGAGCTGTTCATGGATGCGCTAGGGCCCTTCAACTTCGTGCTGGTGAGTTCGGTGAGGATGCAG GGTGTCATCCTGCTGCTGTTCGCCAAGTACTACCACCTGCCCTTCCTGCGAGACGTGCAGACCGACTGC ACGCGCACTGGCCTGGGCGGCTACTGGGGTAACAAGGGTGGCGTGAGCGTGCGCCTGGCGGCCTTCGGG CACATGCTCTGCTTCCTGAACTGCCACTTGCCTGCGCATATGGACAAGGCGGAGCAGCGCAAAGACAAC TTCCAGACCATCCTCAGCCTCCAGCAGTTCCAAGGGCCGGGCGCACAGGGCATCCTGGATCATGACCTC GTGTTCTGGTTCGGGGACCTGAACTTCCGCATTGAGAGCTATGACCTGCACTTTGTCAAGTTTGCCATC GACAGTGACCAGCTCCATCAGCTCTGGGAGAAGGACCAGCTCAACATGGCCAAGAACACCTGGCCCATT CTGAAGGGCTTTCAGGAGGGGCCCCTCAACTTCGCTCCCACCTTCAAGTTTGATGTGGGTACCAACAAA TACGATACCAGTGCCAAGAAACGGAAGCCAGCTTGGACAGACCGTATCCTATGGAAGGTCAAGGCTCCA GGTGGGGGTCCCAGCCCCTCAGGACGGAAGAGCCACCGACTCCAGGTGACGCAGCACAGCTACCGCAGC CACATGGAATACACAGTCAGCGACCACAAGCCTGTGGCTGCCCAGTTCCTCCTGCAGTTTGCCTTCAGG GACGACATGCCACTGGTGCGGCTGGAGGTGGCAGATGAGTGGGTGCGGCCCGAGCAGGCGGTGGTGAGG TACCGCATGGAAACAGTGTTCGCCCGCAGCTCCTGGGACTGGATCGGCTTATACCGGGTGGGTTTCCGC CATTGCAAGGACTATGTGGCTTATGTCTGGGCCAAACATGAAGATGTGGATGGGAATACCTACCAGGTA ACATTCAGTGAGGAATCACTGCCCAAGGGCCATGGAGACTTCATCCTGGGCTACTATAGTCACAACCAC AGCATCCTCATCGGCATCACTGAACCCTTCCAGATCTCGCTGCCTTCCTCGGAGTTGGCCAGCAGCAGC ACAGACAGCTCAGGCACCAGCTCAGAGGGAGAGGATGACAGCACACTGGAGCTCCTTGCACCCAAGTCC CGCAGCCCCAGTCCTGGCAAGTCCAAGCGACACCGCAGCCGCAGCCCGGGACTGGCCAGGTTCCCTGGG CTTGCCCTACGGCCCTCATCCCGTGAACGCCGTGGTGCCAGCCGTAGCCCCTCACCCCAGAGCCGCCGC CTGTCCCGAGTGGCTCCTGACAGGAGCAGTAATGGCAGCAGCCGGGGCAGTAGTGAAGAGGGGCCCTCT GGGTTGCCTGGCCCCTGGGCCTTCCCACCAGCTGTGCCTCGAAGCCTGGGCCTGTTGCCCGCCTTGCGC CTAGAGACTGTAGACCCTGGTGGTGGTGGCTCCTGGGGACCTGATCGGGAGGCCCTGGCGCCCAACAGC CTGTCTCCTAGTCCCCAGGGCCATCGGGGGCTGGAGGAAGGGGGCCTGGGGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001284289
Insert Size	1920 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284289.1
RefSeq Size	2603 bp
RefSeq ORF	1920 bp
Locus ID	27124
UniProt ID	Q15735
Protein Families	Druggable Genome
Protein Pathways	Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	70.5 kDa
Gene Summary	Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in its 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (e) is shorter at the N-terminus, compared to isoform a. Both variants 5 and 6 encode isoform e.

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