COL8A2 (NM_001294347) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FECD; FECD1; PPCD; PPCD2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337068 representing NM_001294347. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTCTACCGCTGCTGCCGATGGACCTGAAGGGAGAGCCCGGCCCCCCTGGGAAGCCCGGGCCTCGG GGTCCCCCTGGCCCCCCTGGCTTCCCAGGAAAACCAGGCATGGGAAAGCCAGGACTCCATGGGCAGCCT GGCCCTGCTGGGCCCCCTGGCTTCTCCCGGATGGGCAAGGCTGGTCCCCCAGGGCTCCCTGGCAAGGTC GGGCCACCAGGGCAGCCGGGGCTTCGGGGGGAGCCAGGAATACGAGGGGACCAGGGCCTCCGGGGACCC CCAGGACCCCCTGGCCTCCCGGGCCCCTCAGGCATTACTATCCCTGGAAAACCAGGTGCCCAAGGGGTG CCAGGGCCCCCAGGATTCCAGGGGGAACCAGGGCCCCAGGGGGAGCCTGGGCCCCCAGGTGATCGAGGC CTCAAGGGGGATAATGGAGTGGGCCAGCCCGGGCTGCCTGGGGCCCCAGGGCAGGGGGGTGCCCCCGGC CCCCCCGGCCTCCCTGGTCCAGCTGGCTTAGGCAAACCTGGTTTGGATGGGCTTCCTGGGGCCCCAGGA GACAAGGGTGAGTCTGGGCCTCCTGGAGTTCCAGGCCCCAGGGGGGAGCCAGGAGCTGTGGGCCCAAAA GGACCTCCTGGAGTAGACGGTGTGGGAGTCCCAGGGGCAGCAGGGTTGCCAGGACCACAGGGCCCATCA GGGGCCAAAGGGGAGCCAGGGACCCGGGGCCCCCCTGGGCTGATAGGCCCCACTGGCTATGGGATGCCA GGACTGCCAGGCCCCAAGGGGGACAGGGGCCCAGCTGGGGTCCCAGGACTCTTGGGGGACAGGGGTGAG CCAGGGGAGGATGGGGAGCCAGGGGAGCAGGGCCCACAGGGTCTTGGGGGTCCCCCTGGACTTCCTGGG TCTGCAGGGCTTCCTGGCAGACGTGGGCCCCCTGGGCCTAAGGGTGAGGCAGGGCCTGGAGGACCCCCA GGAGTGCCTGGCATTCGAGGTGACCAGGGGCCTAGTGGCCTGGCTGGGAAACCAGGGGTCCCAGGTGAG AGGGGACTTCCTGGGGCCCATGGACCCCCTGGACCAACTGGGCCCAAGGGTGAGCCGGGTTTCACGGGT CGCCCTGGAGGACCAGGGGTGGCAGGAGCCCTGGGGCAGAAAGGTGACTTGGGGCTCCCTGGGCAGCCT GGCCTGAGGGGTCCCTCAGGAATCCCAGGACTCCAGGGTCCAGCTGGCCCTATTGGGCCCCAAGGCCTG CCGGGCCTGAAGGGGGAACCAGGCCTGCCAGGGCCCCCTGGAGAGGGGAGAGCAGGGGAACCTGGCACG GCTGGGCCCACGGGGCCCCCAGGGGTCCCTGGCTCCCCTGGAATCACGGGCCCTCCGGGGCCTCCCGGG CCCCCGGGACCCCCTGGTGCCCCTGGGGCCTTCGATGAGACTGGCATCGCAGGCTTGCACCTGCCCAAC GGCGGTGTGGAGGGTGCCGTGCTGGGCAAGGGGGGCAAGCCACAGTTTGGGCTGGGCGAGCTGTCTGCC CATGCCACACCGGCCTTCACTGCGGTGCTCACCTCGCCCTTCCCCGCCTCGGGCATGCCCGTGAAATTT GACCGGACTCTCTACAATGGCCACAGCGGCTACAACCCAGCCACTGGCATCTTCACCTGCCCTGTGGGC GGCGTCTACTACTTTGCTTACCATGTGCACGTCAAGGGCACCAACGTGTGGGTGGCCCTGTACAAGAAC AACGTGCCGGCCACCTATACCTACGATGAGTACAAGAAGGGCTACCTGGACCAGGCATCTGGTGGGGCC GTGCTCCAGCTGCGGCCCAACGACCAGGTCTGGGTGCAGATGCCGTCGGACCAGGCCAACGGCCTCTAC TCCACGGAGTACATCCACTCCTCCTTTTCAGGATTCTTGCTCTGCCCCACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001294347
Insert Size	1917 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001294347.1
RefSeq Size	4397 bp
RefSeq ORF	1917 bp
Locus ID	1296
UniProt ID	P25067
Protein Families	Druggable Genome
MW	60.7 kDa
Gene Summary	This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.

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