CKAP2 (NM_001286686) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LB1; se20-10; TMAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337054 representing NM_001286686. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTATCCAGTAGAGATCAGAGAGTTGTGACATCTGAGGACCAAGTTCAAGAAGGGACTAAAGTGCTG AAACTTAAAACAAAAATGGCTGATAAAGAAAACATGAAGAGACCTGCAGAGAGCAAAAATAATACAGTG GTGGGGAAACATTGTATTCCTTTAAAACCTTCAAATGAACTAACCAATTCAACTGTAGTAATTGACACA CATAAACCTAAGGATAGTAATCAAACTCCGCATTTGTTACTAACTGAAGATGATCCCCAAAGTCAACAT ATGACATTAAGCCAGGCATTTCACCTTAAAAACAATAGTAAAAAGAAACAAATGACTACAGAAAAACAA AAGCAAGATGCTAACATGCCCAAGAAACCTGTGCTTGGATCTTATCGTGGCCAGATTGTTCAGTCTAAG ATTAATTCATTTAGAAAACCTCTACAAGTCAAAGATGAGAGTTCTGCAGCAACAAAGAAACTTTCAGCC ACTATACCTAAAGCCACAAAACCTCAGCCTGTAAACACCAGCAGTGTAACAGTGAAAAGTAATAGATCC TCCAATATGACTGCCACTACTAAATTTGTGAGCACTACATCTCAGAACACACAACTTGTGCGACCTCCT ATTAGAAGTCATCACAGTAATACCCGGGACACTGTGAAACAAGGCATCAGTAGAACTTCTGCCAATGTT ACAATCCGGAAAGGGCCTCATGAAAAAGAACTATTACAATCAAAAACAGCTTTATCTAGTGTCAAAACC AGTTCTTCTCAAGGTATAATAAGAAATAAGACTCTATCAAGATCCATAGCATCTGAAGTTATAGCCAGG CCTGCTTCATTGTCTAATGATAAACTGATGGAAAAGTCAGAGCCCGTTGACCAGCGAAGACATACTGCA GGAAAAGCAATTGTTGATAGTAGATCAGCTCAGCCCAAAGAAACCTCGGAAGAGAGAAAAGCTCGTCTG AGTGAGTGGAAAGCTGGCAAAGGAAGAGTGCTAAAAAGGCCCCCTAATTCAGTAGTTACTCAGCATGAG CCTGCAGGACAAAATGAAAAACCAGTTGGGTCTTTTTGGACTACCATGGCAGAAGAAGATGAACAAAGA TTATTTACTGAAAAAGTAAACAACACATTTTCTGAATGCCTGAACTTGATTAATGAGGGATGTCCAAAA GAAGATATACTGGTCACACTGAATGACCTGATTAAAAATATTCCAGATGCCAAAAAGCTTGTTAAGTAT TGGATATGTCTTGCACTTATTGAACCAATCACAAGTCCTATTGAAAATATTATTGCAATCTATGAGAAA GCCATTCTGGCAGGGGCTCAGCCTATTGAAGAGATGCGACACACGATTGTAGATATTCTAACAATGAAG AGTCAAGAAAAAGCTAATTTAGGAGAAAATATGGAGAAGTCTTGTGCAAGCAAGGAAGAAGTCAAAGAA GTCAGTATTGAAGATACAGGTGTTGATGTAGATCCAGAAAAACTGGAAATGGAGAGTAAACTTCATAGA AATTTGCTATTTCAAGATTGTGAAAAAGAGCAAGACAACAAAACAAAAGATCCAACCCATGATGTTAAA ACCCCCAATACAGAAACGAGGACAAGTTGCTTAATTAAATATAATGTGTCTACTACGCCATACTTGCAA AGTGTGAAAAAAAAGGTGCAGTTTGATGGAACAAATTCCGCATTTAAAGAGCTGAAGTTTTTAACACCA GTGAGACGTTCTCGACGTCTTCAAGAGAAAACTTCTAAATTGCCAGATATGTTAAAAGATCATTATCCT TGTGTGTCTTCATTGGAACAGCTAACGGAGTTGGGAAGAGAAACTGATGCTTTTGTATGCCGCCCTAAT GCAGCACTGTGCCGGGTGTACTATGAGGCTGATACAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286686
Insert Size	1905 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286686.1
RefSeq Size	3677 bp
RefSeq ORF	1905 bp
Locus ID	26586
UniProt ID	Q8WWK9
Protein Families	Druggable Genome
MW	71.2 kDa
Gene Summary	This gene encodes a cytoskeleton-associated protein that stabalizes microtubules and plays a role in the regulation of cell division. The encoded protein is itself regulated through phosphorylation at multiple serine and threonine residues. There is a pseudogene of this gene on chromosome 14. Alternative splicing results in multiple transcript variations. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1.

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