EIF4B (NM_001300821) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIF-4B; PRO1843
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337006 representing NM_001300821. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGACTTTCTGGCTGAG GATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGCTGGGCTGATGAAACGGATGAC CTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGAC CGTTCCATCCTTCCCACTGCTCCACGGGCTGCTCGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAA TCGCCACCCTACACTGCTTTTCTAGGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTC TTTCGAGGATTAAATATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGT TTTGGTTATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAGTCTCTA GGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGGGATGATCGTTCTTTT GGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGGAGGGCTCGTCCTGCTACAGACAGC TTTGATGACTACCCACCTAGAAGAGGTGATGATAGCTTTGGAGACAAGTATCGAGATCGTTATGATTCA GACCGGTATCGGGATGGGTATCGGGATGGGTATCGGGATGGCCCACGCCGGGATATGGATCGATATGGT GGCCGGGATCGCTATGATGACCGAGGCAGCAGAGACTATGATAGAGGCTATGATTCCCGGATAGGCAGT GGCAGAAGAGCATTTGGCAGTGGGTATCGCAGGGATGATGACTACAGAGGAGGCGGGGACCGCTATGAA GACCGATATGACAGACGGGATGATCGGTCGTGGAGCTCCAGAGATGATTACTCTCGGGATGATTATAGG CGTGATGATAGAGGTCCCCCCCAAAGACCCAAACTGAATCTAAAGCCTCGGAGTACTCCTAAGGAAGAT GATTCCTCTGCTAGTACCTCCCAGTCCACTCGAGCTGCTTCTATCTTTGGAGGGGCAAAGCCTGTTGAC ACAGCTGCTAGAGAAAGAGAAGTAGAAGAACGGCTACAGAAGGAACAAGAGAAGTTGCAGCGTCAGCTG GATGAGCCAAAACTAGAACGACGGCCTCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAA CGGGAACGGTCGAGGACAGGAAGTGAGTCATCACAAACTGGGACCTCCACCACATCTAGCAGAAGTAAG TCAGACCAGGATGCACGAAGGAGAGAGAGTGAGAAGTCTCTAGAAAATGAAACACTCAATAAGGAGGAA GATTGCCACTCTCCAACTTCTAAACCTCCCAAACCTGATCAGCCCCTAAAGGTAATGCCAGCCCCTCCA CCAAAGGAGAATGCTTGGGTGAAGCGAAGTTCTAACCCTCCTGCTCGATCTCAGAGCTCAGACACAGAG CAGCAGTCCCCTACAAGTGGTGGGGGAAAAGTAGCTCCAGCTCAACCATCTGAGGAAGGACCAGGAAGG AAAGATGAAAATAAAGTAGATGGGATGAATGCCCCAAAAGGCCAAACTGGGAACTCTAGCCGTGGTCCA GGAGACGGAGGGAACAGAGACCACTGGAAGGAGTCAGATAGGAAAGATGGCAAAAAGGATCAAGACTCC AGATCTGCACCTGAGCCAAAGAAACCTGAGGAAAATCCAGCTTCCAAGTTCAGTTCTGCAAGCAAGTAT GCTGCTCTCTCTGTTGATGGTGAAGATGAAAATGAGGGAGAAGATTATGCCGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300821
Insert Size	1851 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300821.1
RefSeq Size	4076 bp
RefSeq ORF	1851 bp
Locus ID	1975
Protein Pathways	mTOR signaling pathway
MW	69.7 kDa
Gene Summary	Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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