ZNF595 (NM_001286052) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337004 representing NM_001286052. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAACTCGTAACATTCAGGGATGTGGCCATAGAATTCTCCCCTGAAGAGTGGAAATGTCTGGACCCT GCCCAGCAGAATTTGTATAGAGATGTGATGTTGGAGAACTACAGGAACCTGGTCTCCCTGGCTATATGT TCTCCTTTCAGCCAAGACCTTTCACCAGTGCAGGGGATAGAAGATTCATTCCACAAACTTATACTGAAA AGATACGAGAAATGTGGACATGAGAATTTACAATTAAGAAAAGGCTGTAAACGTGTGAATGAGTGTAAG GTGCAGAAAGGAGTTAATAATGGAGTTTACCAGTGCTTGTCAACTACCCAGAGCAAAATATTTCAATGT AATACATGTGTTAAAGTTTTTAGTAAATTTTCAAATTCAAACAAACATAAGATAAGACATACTGGAGAG AAACCCTTTAAATGTACAGAATGTGGCAGATCGTTTTACATGTCACACCTAACTCAACATACAGGAATT CATGCTGGAGAGAAACCCTACAAATGTGAAAAATGTGGCAAAGCCTTTAATAGGTCCACATCACTTAGT AAACATAAGAGAATTCATACTGGAGAGAAACCCTACACATGTGAAGAATGTGGCAAAGCCTTTAGACGG TCCACAGTTCTGAACGAACATAAGAAAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGC AAAGCCTTTACAAGGTCCACAACACTGAATGAACACAAGAAAATTCATACTGGAGAGAAACCCTACAAA TGTAAAGAATGTGGCAAAGCCTTTAGATGGTCCACAAGCCTGAATGAACATAAGAATATTCATACTGGA GAGAAACCCTACAAATGTAAAGAATGTGGCAAAGCCTTTAGACAGTCCAGGAGCCTGAATGAACATAAA AATATTCATACTGGCGAAAAACCCTACACATGTGAAAAATGTGGCAAAGCTTTTAACCAATCCTCAAGT CTTATTATACACAGGAGCATTCATTCTGAACAAAAACTTTACAAATGTGAAGAATGTGGCAAAGCCTTT ACTTGGTCCTCATCCCTTAATAAACATAAGAGAATTCATACTGGAGAGAAACCCTACACATGTGAAGAA TGTGGCAAAGCTTTTTATAGGTCCTCACACCTTGCTAAACATAAGAGAATTCATACTGGAGAGAAACCC TACACGTGCGAAGAATGTGGCAAAGCTTTTAACCAATCCTCAACTCTTATATTACACAAGAGAATCCAT TCTGGGCAAAAACCTTACAAATGTGAAGAATGTGGCAAAGCCTTTACACGGTCCACAACACTGAACGAA CATAAGAAAATTCATACTGGCGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTCATATGGTCC GCAAGCCTGAATGAACATAAGAATATTCATACTGGAGAGAAACCCTACAAATGTAAAGAATGTGGCAAA GCTTTTAACCAATCCTCAGGCCTTATTATACACAGGAGCATTCATTCTGAACAAAAACTTTACAAATGT GAAGAATGTGGCAAAGCCTTTACTCGGTCCACAGCCCTGAATGAACATAAGAAAATTCATTCTGGAGAG AAACCCTACAAATGCAAAGAATGTGGCAAAGCCTATAACTTATCCTCAACCCTTACTAAACATAAGAGA ATTCATACTGGAGAGAAACCCTTCACATGTGAAGAATGTGGCAAAGCCTTCAATTGGTCCTCATCCCTT ACTAAACATAAGATAATTCATACTGGAGAGAAATCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAT CGGCCCTCAACCCTTACTGTACACAAGCGAATTCATACTGGCAAGGAACATAGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286052
Insert Size	1851 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286052.1
RefSeq Size	2815 bp
RefSeq ORF	1851 bp
Locus ID	152687
UniProt ID	Q8IYB9
Protein Families	Transcription Factors
MW	70.8 kDa
Gene Summary	This gene encodes a protein belonging to the Cys2His2 zinc finger protein family, whose members function as transcription factors that can regulate a broad variety of developmental and cellular processes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

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