DGLUCY (NM_001286471) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C14orf159
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC337001 representing NM_001286471. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCTTCACACTCCACCTGAGGTCCCGCCTTCCCTCTGCCATAAGGAGTTTGATTCTACAAAAGAAA CCAAACATCAGAAATACATCCAGCATGGCTGGAGAGCTCCGACCAGCCAGCCTGGTGGTCCTGCCCAGG TCCCTTGCTCCAGCTTTTGAAAGATTCTGCCAGGTCAACACTGGTCCTCTACCCCTGCTGGGCCAGAGT GAGCCAGAAAAGTGGATGCTGCCCCCTCAAGGTGCTATCTCAGAGACCAGGATGGGCCATCCCCAGTTC TGGAAATACGAGTTCGGTGCCTGCACCGGCAGCCTGGCTTCGCTGGAGCAGTACTCGGAGCAGCTGAAG GACATGGTGGCCTTCTTCCTGGGCTGCAGCTTCTCCCTGGAGGAGGCCTTGGAGAAAGCGGGGCTCCCC AGAAGAGACCCAGCAGGTCACAGCCAGACAACAGTGCCTTGTGTTACCCATGCTGGCTTCTGCTGCCCT CTGGTGGTCACGATGAGGCCCATTCCCAAGGACAAGCTGGAAGGGCTGGTGCGGGCCTGCTGCTCCCTC GGAGGTGAGCAGGGGCAACCTGTTCACATGGGCGACCCAGAACTGTTGGGAATCAAAGAGCTTTCCAAA CCTGCCTACGGGGATGCCATGGTGTGTCCCCCAGGGGAGGTTCCAGTGTTCTGGCCTTCTCCGCTGACC AGTCTCGGAGCTGTCAGCAGCTGTGAGACCCCACTGGCTTTTGCCAGCATCCCAGGCTGCACAGTTATG ACTGACCTGAAGGATGCAAAGGCTCCACCTGGTTGTCTCACCCCAGAGAGAATTCCAGAGGTCCATCAC ATTTCCCAAGATCCTCTGCACTACAGCATCGCGTCAGTCTCTGCTTCTCAGAAGATCAGAGAACTAGAG TCTATGATCGGCATAGACCCAGGGAACCGGGGGATTGGGCACCTGCTCTGTAAAGATGAGCTGCTGAAG GCCTCTCTCTCGCTGTCCCATGCCCGCTCAGTGCTCATCACCACTGGGTTCCCCACACATTTCAATCAT GAGCCTCCAGAAGAGACAGATGGCCCACCAGGAGCTGTTGCTCTGGTTGCCTTCCTGCAGGCCTTGGAG AAGGAGGTCGCCATAATCGTTGACCAGAGAGCCTGGAACTTGCACCAGAAGATTGTTGAAGATGCTGTT GAGCAAGGTGTTCTGAAGACGCAGATCCCGATATTAACTTACCAAGGTGGATCAGTGGAAGCTGCTCAG GCATTCCTGTGCAAAAATGGGGACCCGCAGACACCTAGATTTGACCACCTGGTGGCCATAGAGCGTGCC GGAAGAGCTGCTGATGGCAATTACTACAATGCAAGGAAGATGAACATCAAGCACTTGGTTGACCCCATT GACGATCTTTTTCTTGCTGCGAAGAAGATTCCTGGAATCTCATCAACTGGAGTCGGTGATGGAGGCAAC GAGCTTGGGATGGGTAAAGTCAAGGAGGCTGTGAGGAGGCACATACGGCACGGGGATGTCATCGCCTGC GACGTGGAGGCTGACTTTGCCGTCATTGCTGGTGTTTCTAACTGGGGAGGCTATGCCCTGGCCTGCGCA CTCTACATCCTGTACTCATGTGCTGTCCACAGTCAGTACCTGAGGAAAGCAGTCGGACCCTCCAGGGCA CCTGGAGATCAGGCCTGGACTCAGGCCCTCCCGTCGGTCATTAAGGAAGAAAAAATGCTGGGCATCTTG GTGCAGCACAAAGTCCGGAGTGGCGTCTCGGGCATCGTGGGCATGGAGGTGGATGGGCTGCCCTTCCAC AACACCCACGCCGAGATGATCCAGAAGCTGGTGGACGTCACCACGGCACAGGTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286471
Insert Size	1851 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286471.1
RefSeq Size	3017 bp
RefSeq ORF	1851 bp
Locus ID	80017
UniProt ID	Q7Z3D6
MW	66.4 kDa
Gene Summary	D-glutamate cyclase that converts D-glutamate to 5-oxo-D-proline.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 7 encode the same isoform (a).

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