ZNF189 (NM_001278231) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336979 representing NM_001278231. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTTCCCCGAGCCCCCCGCCGGAGTCGAAGGAGGAGTGGGATTATCTGGACCCAGCTCAGAGAAGC CTGTATAAAGATGTCATGATGGAGAATTATGGAAACCTGGTCTCACTGGATGTTTTGAACAGAGATAAG GATGAGGAGCCAACTGTAAAACAAGAGATTGAAGAAATTGAGGAAGAAGTGGAACCACAGGGTGTAATA GTTACAAGAATCAAAAGTGAAATTGACCAGGATCCTATGGGTAGAGAAACATTTGAACTTGTTGGTAGG TTAGATAAACAAAGAGGGATCTTCCTATGGGAAATACCAAGGGAATCTTTGACCCAGGAACAGAGAATG TTCAGAGAAAACACTAACATTATCCGTAAAAGACCAAACTCAGAAGAGAAATGCCATAAATGTGAAGAA TGTGGAAAGGGTTTTGTCCGCAAGGCCCATTTCATTCAACATCAAAGGGTCCATACTGGTGAGAAACCT TTTCAGTGCAATGAATGTGGGAAAAGTTTTAGTCGCAGTTCATTTGTTATTGAACATCAGAGAATTCAC ACTGGGGAAAGGCCCTATGAGTGTAATTACTGTGGAAAAACCTTTAGTGTGAGCTCAACCCTTATTAGA CATCAGAGAATCCACACTGGAGAAAGACCCTATCAGTGTAATCAGTGTAAACAGAGCTTCAGCCAGAGA AGGAGCCTTGTTAAACATCAAAGGATTCATACAGGTGAGAAACCCCATAAATGTAGTGACTGTGGGAAA GCCTTCAGTTGGAAATCACACCTTATTGAGCATCAAAGAACTCACACTGGTGAGAAACCTTATCACTGT ACCAAATGTAAGAAGAGCTTTAGTCGAAATTCATTGCTTGTTGAGCATCAAAGAATTCACACTGGGGAA AGACCCCATAAATGTGGTGAATGTGGGAAAGCCTTTCGATTAAGCACATACCTTATACAACACCAAAAA ATTCACACTGGCGAGAAGCCTTTTCTTTGTATTGAGTGTGGAAAAAGTTTCAGTCGGAGCTCATTCCTT ATTGAACATCAGAGGATCCATACTGGTGAAAGACCTTATCAGTGCAAAGAGTGTGGGAAAAGTTTCAGT CAGCTTTGCAACCTTACTCGTCATCAGAGAATTCACACAGGAGACAAGCCCCATAAATGTGAGGAATGT GGAAAAGCCTTTAGTAGAAGCTCAGGTCTTATTCAGCATCAGAGAATTCACACCAGGGAGAAGACTTAT CCATACAATGAAACTAAGGAAAGTTTTGATCCAAATTGCAGTCTTGTTATACAGCAGGAAGTCTACCCT AAGGAGAAATCTTATAAATGTGATGAATGTGGGAAAACTTTTAGTGTTAGTGCTCATCTTGTACAACAT CAAAGAATCCACACTGGTGAAAAGCCCTATCTATGTACTGTCTGTGGGAAAAGCTTCAGCCGGAGCTCA TTTCTTATTGAACATCAGAGAATCCACACTGGTGAGAGACCCTATCTGTGCAGACAGTGTGGAAAAAGC TTTAGTCAGCTTTGTAATCTTATTCGACATCAGGGTGTTCACACAGGTAATAAACCCCATAAATGTGAT GAATGTGGAAAGGCCTTTAGCCGGAACTCGGGTCTTATTCAGCATCAGAGAATACACACAGGAGAGAAA CCTTATAAGTGTGAGAAGTGCGACAAAAGTTTCAGTCAACAGCGCAGTCTTGTCAACCATCAGAAGATC CATGCAGAGGTGAAAACCCAAGAAACCCATGAATGTGACGCTTGTGGTGAAGCCTTTAATTGCCGTATT TCTCTTATTCAGCATCAGAAATTGCACACAGCATGGATGCAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278231
Insert Size	1839 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278231.1
RefSeq Size	3171 bp
RefSeq ORF	1839 bp
Locus ID	7743
UniProt ID	O75820
Protein Families	Transcription Factors
MW	71.4 kDa
Gene Summary	Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010] Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1.

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