ANKLE1 (NM_001278445) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANKRD41; LEM3; LEMD6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336974 representing NM_001278445. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCACAAGCCCCCCGCGGGAAGTAGGAAATCGACCGACCAGGCGGTGCGCTTCGGACCCAGCCAGG GCATGTGCTCGGAGGCCCGCCTGGCTCGCAGGTTGCGGGATGCGCTGCGGGAGGAGGAGCCGTGTAGAG GAGCTGCTGCGCTGCGGCGCGGACCCTAATTTGGTGCTAGAGGACGGCGCAGCGGCTGTGCACTTGGCG GCCGGAGCCCGGCACCCGCGCGGCCTGCGTTGCCTCGGGGCCCTACTGCGCCAAGGCGGGGACCCCAAC GCTCGATCTGTCGAGGCACTGACGCCGCTGCATGTGGCCGCCGCGTGGGGCTGCCGCCGCGGCCTGGAG CTGCTGCTGAGCCAAGGAGCGGACCCGGCGCTGCGCGACCAGGACGGACTCCGGCCGCTGGACCTGGCC CTGCAGCAGGGACACCTGGAGTGCGCGCGAGTCCTGCAGGATCTCGACACGCGGACCAGGACCCGGACC CGGATCGGGGCAGAGACTCAGGAGCCCGAGCCTGCACCTGGCACCCCAGGCCTCTCTGGACCTACCGAT GAGACGCTGGACTCCATAGCACTCCAAAAGCAGCCATGCAGAGGTGACAACAGGGACATTGGCTTGGAG GCTGACCCAGGACCCCCCAGCCTCCCTGTTCCCCTTGAAACTGTGGACAAACATGGGAGCTCGGCGTCC CCTCCAGGGCACTGGGATTACAGCTCAGACGCCTCTTTCGTCACAGCGGTTGAGGTCTCTGGAGCTGAG GACCCAGCCTCGGACACTCCCCCCTGGGCTGGGTCATTGCCACCGACCAGGCAGGGACTTCTGCATGTT GTCCATGCCAACCAGAGGGTACCTAGGTCTCAGGGCACGGAGGCAGAACTGAATGCCCGTCTGCAGGCC CTGACTCTGACCCCACCAAATGCTGCTGGCTTCCAGTCCTCCCCTTCCTCCATGCCTCTCCTGGACAGG AGTCCAGCTCATAGCCCCCCACGGACACCAACCCCTGGAGCTTCTGACTGCCACTGCCTGTGGGAGCAC CAGACATCCATTGATAGTGACATGGCCACGCTCTGGCTGACAGAGGATGAGGCAAGCTCTACAGGTGGC AGGGAACCTGTCGGCCCTTGCCGGCACCTGCCAGTCTCCACTGTGTCTGACTTGGAGTTGCTGAAGGGA CTCCGAGCACTTGGCCCAGAGTTTTCAGGGCACAGCCTAGAACTGGCTGCAGCCCTGCGGACGGGCTGT ATTCCAGATGTCCAGGCAGATGAAGACGCGCTGGCCCAGCAGTTTGAGCAGCCAGATCCTGCCAGGAGG TGGCGGGAGGGGGTCGTGAAGTCTAGCTTCACCTATCTGCTGCTGGACCCCAGGGAGACTCAGGACCTG CCAGCCCGAGCCTTCTCACTGACCCCAGCTGAGCGCCTTCAGACTTTCATCCGTGCCATCTTCTACGTG GGCAAAGGGACGAGGGCCCGGCCATATGTCCACCTCTGGGAGGCCCTTGGTCACCATGGGCGGTCAAGA AAACAGCCCCACCAGGCCTGCCCCAAGGTGCGTCAGATCTTGGACATCTGGGCCAGTGGTTGCGGTGTT GTGTCCCTACATTGCTTCCAGCACGTGGTCGCTGTGGAGGCTTATACACGGGAGGCGTGTATTGTGGAA GCCCTAGGGATCCAGACGCTCACCAACCAGAAGCAAGGGCACTGCTATGGAGTGGTGGCAGGCTGGCCA CCTGCTCGTCGCCGGCGCTTGGGGGTGCACCTGCTGCACCGTGCCCTCCTTGTCTTCCTGGCTGAAGGC GAGCGACAGCTTCATCCCCAGGACATCCAGGCCCGGGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278445
Insert Size	1836 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278445.1
RefSeq Size	3135 bp
RefSeq ORF	1836 bp
Locus ID	126549
UniProt ID	Q8NAG6
MW	66.1 kDa
Gene Summary	Endonuclease that probably plays a role in the DNA damage response and DNA repair.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses two alternate splice sites in the coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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