EBF1 (NM_001290360) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	COE1; EBF; O/E-1; OLF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336912 representing NM_001290360. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCAGCATGAAGGAAGAGCCGCTGGGCAGCGGC ATGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACGGCGGCGCAGAGCGGG GTGGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCCAATCTGCGGAAATCCAACTTCTTCCAC TTCGTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATCGAGAGGACAGCGTTTGTGGGGTTC GTGGAGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAACGGAATTCACTACCGGCTTCAGCTTCTC TACAGCAATGGGATAAGGACGGAGCAGGATTTCTACGTGCGCCTCATTGACTCCATGACAAAACAAGCC ATAGTGTATGAAGGCCAAGACAAGAACCCAGAAATGTGCCGAGTCTTGCTCACACATGAGATCATGTGC AGCCGCTGTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTGAC AGGTTCTTCTTGAAATTTTTCCTCAAATGTAACCAAAATTGCCTAAAGAATGCGGGAAACCCACGTGAC ATGCGGAGATTCCAGGTCGTGGTGTCTACGACAGTCAATGTGGATGGCCATGTCCTGGCAGTCTCTGAT AACATGTTTGTCCATAATAATTCCAAGCATGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACG CCCTCTTATCTGGAACATGCAGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACG GGAGGTGCGACTGTGATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATG CTGGTCTGGAGTGAGTTGATCACTCCTCATGCCATCCGTGTGCAGACCCCTCCTCGGCACATCCCTGGT GTTGTGGAAGTCACACTGTCCTACAAATCTAAGCAGTTCTGCAAAGGAACACCAGGCAGATTCATTTAT ACAGCGCTCAACGAACCCACCATCGATTATGGTTTCCAGAGGTTACAGAAGGTCATTCCTCGGCACCCT GGTGACCCTGAGCGTTTGCCAAAGGAAGTAATACTCAAAAGGGCTGCGGATCTGGTAGAAGCACTGTAT GGGATGCCACACAACAACCAGGAAATCATTCTGAAGAGAGCGGCCGACATTGCCGAGGCCCTGTACAGT GTTCCCCGCAACCACAACCAACTCCCGGCCCTTGCTAACACCTCGGTCCACGCAGGGATGATGGGCGTG AATTCGTTCAGTGGACAACTGGCCGTGAATGTCTCCGAGGCATCACAAGCCACCAATCAGGGTTTCACC CGCAACTCAAGCAGCGTATCACCACACGGGTACGTGCCGAGCACCACTCCCCAGCAGACCAACTATAAC TCCGTCACCACGAGCATGAACGGATACGGCTCTGCCGCAATGTCCAATTTGGGCGGCTCCCCCACCTTC CTCAACGGCTCAGCTGCCAACTCCCCCTATGCCATAGTGCCATCCAGCCCCACCATGGCCTCCTCCACA AGCCTCCCCTCCAACTGCAGCAGCTCCTCGGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCC GTGAAACAGAAGAGTGCTTTCGCACCAGTCGTCAGACCCCAGACCTCCCCACCTCCCACCTGCACCAGC ACCAACGGGAACAGCCTGCAAGCGATATCTGGCATGATTGTTCCTCCTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290360
Insert Size	1779 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290360.2
RefSeq Size	5267 bp
RefSeq ORF	1779 bp
Locus ID	1879
Protein Families	Transcription Factors
MW	64.5 kDa
Gene Summary	Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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