CPSF6 (NM_001300947) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336896 representing NM_001300947. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGACGGCGTGGACCACATAGACATTTACGCGGATGTCGGCGAAGAGTTCAACCAGGAAGCTGAA TATGGTGGGCATGATCAGATAGATTTGTATGACGATGTCATATCTCCATCTGCAAATAATGGAGATGCC CCAGAAGACCGAGATTACATGGATACTCTCCCACCAACTGTTGGTGATGATGTGGGTAAAGGAGCAGCA CCAAATGTTGTCTATACATATACTGGAAAGAGAATTGCATTATATATTGGAAATCTAACATGGTGGACA ACAGATGAAGACTTAACTGAAGCAGTTCATTCTTTGGGAGTAAATGATATTTTGGAGATAAAATTTTTT GAAAATCGGGCAAATGGCCAGTCAAAGGGGTTTGCCCTTGTTGGTGTTGGATCTGAAGCATCTTCAAAA AAGTTAATGGATCTGTTACCTAAAAGAGAACTTCATGGTCAGAATCCTGTTGTAACTCCATGCAATAAA CAGTTCCTGAGTCAATTTGAAATGCAGTCCAGGAAAACTACACAATCAGGACAAATGTCTGGGGAAGGT AAAGCTGGTCCTCCAGGAGGCAGTTCCCGTGCAGCATTTCCACAAGGTGGTAGAGGACGGGGCCGTTTT CCAGGGGCTGTTCCTGGTGGGGACAGATTTCCTGGGCCAGCAGGACCAGGAGGGCCACCCCCACCTTTT CCAGGAAATTTGATCAAGCATCTTGTTAAAGGAACTCGGCCTTTGTTCCTGGAAACTAGGATTCCATGG CATATGGGGCACAGCATAGAGGAAATACCCATTTTTGGCCTAAAAGCTGGACAGACTCCACCACGTCCA CCCTTAGGTCCTCCAGGCCCACCTGGTCCACCAGGTCCTCCACCTCCTGGTCAGGTTCTGCCTCCTCCT CTAGCTGGGCCTCCTAATCGAGGAGATCGCCCTCCACCACCAGTTCTTTTTCCTGGACAACCTTTTGGG CAGCCTCCATTGGGTCCACTTCCTCCTGGCCCTCCACCTCCAGTTCCAGGCTACGGCCCCCCTCCTGGC CCACCACCTCCACAACAGGGACCACCTCCACCTCCAGGCCCCTTTCCACCTCGTCCACCCGGTCCACTT GGGCCACCCCTTACACTAGCTCCTCCTCCGCATCTTCCTGGACCACCTCCAGGTGCCCCACCGCCAGCT CCGCATGTGAACCCAGCTTTCTTTCCTCCACCAACTAACAGTGGCATGCCTACATCAGATAGCCGAGGT CCACCACCAACAGATCCATATGGGCGACCTCCACCATATGATAGGGGTGACTATGGCCCCCCTGGAAGG GAAATGGATACTGCAAGAACGCCATTGAGTGAAGCTGAATTTGAAGAAATCATGAATAGAAATAGGGCA ATCTCAAGCAGTGCTATTTCGAGAGCTGTGTCTGATGCCAGTGCTGGTGATTATGGGAGTGCTATTGAG ACACTGGTAACTGCAATTTCTTTAATTAAACAATCCAAAGTATCTGCTGATGATCGTTGCAAAGTTCTT ATTAGTTCTTTGCAAGATTGCCTTCATGGAATTGAGTCCAAGTCTTATGGTTCTGGATCAAGACGTGAA CGATCAAGAGAGAGGGACCATAGTAGATCACGAGAAAAGAGTCGACGTCATAAATCCCGTAGTAGAGAC CGTCATGACGATTATTACAGAGAGAGAAGCAGAGAACGAGAGAGGCACCGGGATCGTGACCGAGACCGT GACCGAGAGCGTGACCGAGAGCGCGAATATCGTCATCGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300947
Insert Size	1767 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300947.1
RefSeq Size	6741 bp
RefSeq ORF	1767 bp
Locus ID	11052
UniProt ID	Q16630
MW	63.5 kDa
Gene Summary	The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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