EYA4 (NM_001301012) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMD1J; DFNA10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336883 representing NM_001301012. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGACTCCCAGGATTTAAATGAACAATCAGTAAAGAAAACGTGCACAGAATCAGATGTTTCACAA TCTCAGAATTCCAGGTCTATGGAAATGCAGGACCTAGCAAGTCCTCATACTCTTGTTGGAGGTGGTGAT ACTCCAGGTAGCTCCAAACTGGAAAAATCTAATCTCAGCAGCACATCAGTTACTACAAATGGGACAGGA GTAATTACAAGTAGTGGCTACAGCCCCAGATCAGCACATCAGTATTCCCCACAGCTGTATCCTTCCAAG CCCTATCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCTGCCTATGCAGGCCAGACTCAGTAT TCGGGGATGCAGCAGCCAGCCGTCTACACAGCCTACTCACAGACAGGACAGCCCTACAGCTTGCCCACT TACGATTTGGGTGTGATGTTGCCAGCCATCAAGACAGAGAGTGGACTTTCCCAAACTCAGTCCCCATTA CAGAGTGGCTGCCTCAGTTACAGCCCAGGGTTCTCTACCCCACAGCCAGGCCAGACACCTTATTCTTAC CAAATGCCAGGTTCTAGTTTTGCACCATCATCTACTATTTATGCAAATAATTCAGTTTCCAATTCAACG AATTTCAGTGGTTCACAACAGGATTATCCATCCTATACAGCCTTTGGCCAAAACCAGTATGCACAGTAT TATTCAGCATCAACGTATGGAGCGTATATGACATCGAATAACACAGCCGATGGCACACCCTCTTCAACC TCTACTTATCAGTTGCAGGAATCTCTCCCAGGACTGACTAACCAACCAGGAGAGTTCGATACCATGCAG AGTCCCTCCACACCCATCAAAGATCTTGATGAGAGAACCTGTAGGAGTTCTGGGTCAAAGTCCAGAGGA AGAGGCCGGAAAAATAATCCCTCCCCGCCTCCTGATAGTGACCTGGAGCGTGTGTTTGTCTGGGATTTG GATGAAACCATCATTGTTTTTCACTCACTGCTCACCGGGTCTTATGCACAGAAGTATGGCAAGGATCCC CCCATGGCTGTAACCCTTGGACTCCGCATGGAAGAAATGATTTTTAATCTTGCTGATACTCATTTGTTT TTTAATGATTTAGAGGAGTGTGATCAAGTTCATATAGATGATGTTTCCTCTGATGATAATGGGCAGGAC TTAAGTACCTACAGTTTTGCAACTGATGGCTTCCATGCAGCTGCAAGTAGTGCAAACCTTTGTTTGCCA ACAGGTGTAAGAGGAGGGGTTGACTGGATGAGGAAGTTGGCTTTTCGTTACAGAAGAGTAAAAGAATTA TATAACACCTACAAGAACAACGTTGGAGGACTCCTTGGCCCTGCCAAGAGGGATGCCTGGCTACAGTTA AGGGCAGAGATTGAAGGTCTGACAGATTCCTGGCTAACAAATGCACTTAAGTCTTTATCAATTATTAGC ACTAGGAGTAACTGCATAAATGTCTTGGTAACGACAACTCAACTGATCCCAGCACTTGCGAAGGTTCTA CTCTATAGTTTAGGAGGTGCTTTCCCCATTGAGAATATTTACAGTGCAACTAAAATAGGCAAGGAAAGC TGTTTTGAGCGTATAGTGTCCAGATTTGGCACTAACATAACTTATGTTGTGATTGGAGATGGCCGAGAT GAGGAGCATGCCGCTAACCAGCACAACATGCCCTTCTGGAGGATATCCAGTCACTCAGACCTCCTGGCT CTCCACCAAGCACTGGAATTAGAGTATTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301012
Insert Size	1758 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301012.1
RefSeq Size	5393 bp
RefSeq ORF	1758 bp
Locus ID	2070
UniProt ID	O95677
Protein Families	Druggable Genome, Phosphatase, Transcription Factors
MW	63.9 kDa
Gene Summary	This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (5) has multiple differences in its coding region, compared to variant 1. The resulting isoform (e) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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