DGLUCY (NM_001286472) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C14orf159
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336870 representing NM_001286472. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCTTCACACTCCACCTGAGGTCCCGCCTTCCCTCTGCCATAAGGAGTTTGATTCTACAAAAGAAA CCAAACATCAGAAATACATCCAGCATGGCTGGAGAGCTCCGACCAGCCAGCCTGGTGGTCCTGCCCAGG TCCCTTGCTCCAGCTTTTGAAAGATTCTGCCAGGTCAACACTGGTCCTCTACCCCTGCTGGGCCAGAGT GAGCCAGAAAAGTGGATGCTGCCCCCTCAAGGTGCTATCTCAGAGACCAGGATGGGCCATCCCCAGTTC TGGAAATACGAGTTCGGTGCCTGCACCGGCAGCCTGGCTTCGCTGGAGCAGTACTCGGAGCAGCTGAAG GACATGGTGGCCTTCTTCCTGGGCTGCAGCTTCTCCCTGGAGGAGGCCTTGGAGAAAGCGGGGCTCCCC AGAAGAGACCCAGCAGGTCACAGCCAGGCGGGTGCATACAAGACAACAGTGCCTTGTGTTACCCATGCT GGCTTCTGCTGCCCTCTGGTGGTCACGATGAGGCCCATTCCCAAGGACAAGCTGGAAGGGCTGGTGCGG GCCTGCTGCTCCCTCGGAGGTGAGCAGGGGCAACCTGTTCACATGGGCGACCCAGAACTGTTGGGAATC AAAGAGCTTTCCAAACCTGCCTACGGGGATGCCATGGTGTGTCCCCCAGGGGAGGTTCCAGTGTTCTGG CCTTCTCCGCTGACCAGTCTCGGAGCTGTCAGCAGCTGTGAGACCCCACTGGCTTTTGCCAGCATCCCA GGCTGCACAGTTATGACTGACCTGAAGGATGCAAAGGCTCCACCTGGTTGTCTCACCCCAGAGAGAATT CCAGAGGTCCATCACATTTCCCAAGATCCTCTGCACTACAGCATCGCGTCAGTCTCTGCTTCTCAGAAG ATCAGAGAACTAGAGTCTATGATCGGCATAGACCCAGGGAACCGGGGGATTGGGCACCTGCTCTGTAAA GATGAGCTGCTGAAGGCCTCTCTCTCGCTGTCCCATGCCCGCTCAGTGCTCATCACCACTGGGTTCCCC ACACATTTCAATCATGAGCCTCCAGAAGAGACAGATGGCCCACCAGGAGCTGTTGCTCTGGTTGCCTTC CTGCAGGCCTTGGAGAAGGAGGTCGCCATAATCGTTGACCAGAGAGCCTGGAACTTGCACCAGAAGATT GTTGAAGATGCTGTTGAGCAAGGTGTTCTGAAGACGCAGATCCCGATATTAACTTACCAAGGTGGATCA GTGGAAGCTGCTCAGGCATTCCTGTGCAAAAATGGGGACCCGCAGACACCTAGATTTGACCACCTGGTG GCCATAGAGCGTGCCGGAAGAGCTGCTGATGGCAATTACTACAATGCAAGGAAGATGAACATCAAGCAC TTGGTTGACCCCATTGACGATCTTTTTCTTGCTGCGAAGAAGATTCCTGGAATCTCATCAACTGGTGTT TCTAACTGGGGAGGCTATGCCCTGGCCTGCGCACTCTACATCCTGTACTCATGTGCTGTCCACAGTCAG TACCTGAGGAAAGCAGTCGGACCCTCCAGGGCACCTGGAGATCAGGCCTGGACTCAGGCCCTCCCGTCG GTCATTAAGGAAGAAAAAATGCTGGGCATCTTGGTGCAGCACAAAGTCCGGAGTGGCGTCTCGGGCATC GTGGGCATGGAGGTGGATGGGCTGCCCTTCCACAACACCCACGCCGAGATGATCCAGAAGCTGGTGGAC GTCACCACGGCACAGGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286472
Insert Size	1746 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286472.1
RefSeq Size	2832 bp
RefSeq ORF	1746 bp
Locus ID	80017
UniProt ID	Q7Z3D6
MW	62.8 kDa
Gene Summary	D-glutamate cyclase that converts D-glutamate to 5-oxo-D-proline.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (8) differs in the 5' UTR, uses an alternate in-frame splice site, and lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (d) is shorter than isoform a.

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