Myelin expression factor 2 (MYEF2) (NM_001301210) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HsT18564; MEF-2; MST156; MSTP156; myEF-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336852 representing NM_001301210. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGACGCCAACAAGGCCGAGGTGCCCGGGGCCACTGGTGGCGACAGCCCGCACCTGCAGCCCGCA GAGCCGCCGGGCGAGCCGCGGCGAGAGCCGCACCCCGCGGAGGCGGAGAAGCAGCAGCCGCAGCACAGC AGCAGCTCCAATGGCGTTAAAATGGAGAATGATGAATCAGCAAAAGAAGAGAAATCTGACTTAAAGGAA AAATCTACAGGAAGTAAGAAGGCCAATAGATTTCATCCTTATTCAAAAGACAAGAATTCGGGCACTGGA GAAAAGAAGGGTCCAAATCGTAACAGAGTTTTCATTAGCAACATCCCATATGACATGAAATGGCAAGCT ATTAAAGATCTAATGAGAGAGAAAGTTGGTGAGGTTACATACGTGGAGCTCTTTAAGGATGCGGAAGGA AAATCAAGGGGTTGTGGTGTGGTTGAATTCAAAGATGAAGAATTTGTAAAGAAAGCCCTAGAAACTATG AACAAATATGATCTTAGTGGAAGACCCCTTAATATTAAAGAGGATCCTGATGGAGAAAATGCTCGTAGG GCATTGCAGCGAACAGGAGGATCATTTCCAGGAGGACACGTCCCTGATATGGGATCAGGGTTGATGAAT TTACCACCTTCCATACTCAATAATCCAAACATTCCTCCTGAAGTCATCAGTAATTTGCAGGCCGGTAGA CTTGGTTCCACAATTTTTGTTGCCAATCTTGACTTCAAAGTTGGTTGGAAGAAGCTAAAGGAAGTGTTC AGCATAGCTGGAACTGTGAAGCGGGCAGATATTAAAGAAGACAAAGATGGCAAGAGCAGAGGAATGGGC ACTGTCACTTTTGAGCAAGCAATTGAAGCAGTTCAAGCAATTTCTATGTTCAATGGGCAGTTTTTATTT GATAGACCTATGCATGTGAAAATGGATGACAAGTCTGTTCCTCATGAAGAGTACCGTTCACATGATGGT AAAACACCACAATTACCACGTGGTCTTGGAGGCATTGGGATGGGACTTGGTCCGGGTGGACAGCCTATT AGTGCCAGCCAGTTGAACATAGGTGGAGTAATGGGAAATTTAGGTCCAGGTGGTATGGGAATGGATGGT CCAGGTTTTGGAGGAATGAATAGAATTGGAGGAGGAATAGGGTTTGGTGGTCTGGAAGCAATGAATAGC ATGGGAGGATTTGGAGGAGTTGGCCGAATGGGAGAGCTGTACCGTGGTGCGATGACTAGTAGCATGGAG CGAGATTTTGGACGTGGTGATATTGGAATAAATCGAGGCTTTGGAGATTCCTTTGGTAGACTTGGTGGT GGAATGGGTAGCATGAACAGTGTGACTGGAGGAATGGGGATGGGACTGGACCGGATGAGTTCCAGCTTT GATAGAATGGGACCAGGTATAGGAGCTATACTGGAAAGGAGCATCGATATGGATCGAGGATTTTTATCG GGTCCAATGGGAAGCGGAATGAGAGAGAGAATAGGCTCCAAAGGCAACCAGATATTTGTCAGAAATCTA CCTTTTGACTTGACTTGGCAGAAACTAAAAGAGAAATTCAGTCAGTGTGGTCATGTAATGTTTGCAGAA ATAAAAATGGAGAATGGAAAGTCAAAAGGCTGTGGAACAGTCAGATTTGACTCCCCAGAATCAGCTGAA AAAGCCTGCAGAATAATGAATGGCATAAAAATCAGTGGCAGAGAAATTGATGTTCGCTTGGATCGTAAT GCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301210
Insert Size	1731 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301210.1
RefSeq Size	5335 bp
RefSeq ORF	1731 bp
Locus ID	50804
UniProt ID	Q9P2K5
Protein Families	Transcription Factors
MW	62 kDa
Gene Summary	Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

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