ZNF417 (NM_001297734) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336842 representing NM_001297734. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGCGGCTGCGCCGAGGCGCCCGACTCAGGGCACTGTGACCTTTGAAGATGTGGCTGTGAACTTT TCCCAGGAGGAGTGGTGTCTTCTTAGTGAGGCTCAGAGGTGCTTGTACCGTGATGTGATGCTAGAGAAC CTGGCTCTCATATCCTCGCTGGGTTGTTGGTGTGGATCAAAAGATGAGGAGGCACCTTGTAAGCAGAGA ATTTCTGTACAAAGAGAGTCTCAGAGCAGGACTCCTAGGGCAGGTGTTTCTCCTAAGAAGGCTCACCCC TGTGAAATGTGTGGCCTCATCTTGGAGGATGTTTTTCACTTTGCTGACCACCAGGAAACTCATCACAAG CAGAAGCTGAACAGGAGTGGAGCATGTGGAAAAAACTTGGATGACACTGCATACCTTCATCAGCACCAG AAGCAGCATATTGGAGAGAAATTCTACAGAAAGAGTGTCAGAGAAGCATCGTTTGTAAAGAAACGTAAG CTCAGGGTGTCACAGGAGCCATTTGTCTTCCGCGAGTTTGGGAAGGACGTTCTGCCCAGTTCAGGATTG TGCCAAGAAGCAGCTGCTGTAGAGAAGACAGACAGTGAAACTATGCATGGCCCACCCTTTCAGGAGGGA AAAACTAATTACAGTTGTGGAAAACGCACAAAAGCCTTCAGCACCAAACACTCAGTTATTCCACACCAG AAACTTTTCACTAGAGATGGATGTTATGTGTGCAGTGATTGTGGAAAATCCTTTAGCAGATATGTCAGC TTCAGTAATCATCAGCGAGATCACACTGCAAAAGGACCTTATGATTGTGGAGAGTGTGGGAAATCTTAT AGTCGAAAGAGCAGCCTTATTCAACATCAGCGAGTCCACACTGGAAAGACAGCTTATCCCTGTGAGGAG TGCGGGAAATCTTTTAGTCAGAAGGGCAGCCTTATTAGCCATCAGCGTGTTCACACTGGAGAAAGGCCT TATGAGTGTAGAGAATATGGGAAATCTTTTGGTCAAAAGGGTAACCTCATTCAACATCAGCAAGGTCAC ACTGGAGAGAGAGCTTATCACTGTGGGGAATGTGGGAAATCTTTTCGTCAGAAGTTCTGCTTTATTAAC CATCAGCGTGTTCACACTGGAGAAAGGCCTTACAAGTGTGGAGAATGTGGAAAATCTTTTGGTCAAAAG GGCAACCTCGTTCAACATCAGCGAGGTCATACTGGAGAAAGGCCCTATGAGTGCAAGGAATGTGGGAAA TCATTTAGGTACAGATCCCACCTCACTGAACACCAGAGACTTCACACTGGGGAAAGACCTTACAATTGT AGGGAATGTGGGAAATTATTTAACAGGAAGTATCATCTTCTCGTTCATGAGAGAGTTCACACTGGAGAA AGGCCATATGCGTGTGAGGTATGTGGGAAATTATTTGGTAATAAGAACTGCGTGACTATACATCAGAGG ATTCACACTGGAGAAAGGCCGTATGAATGCAATGAATGTGGGAAATCATTTCTTTCCAGCTCTGCGCTT CATGTTCATAAAAGAGTTCATTCTGGACAAAAGCCTTATAAGTGCAGTGAATGTGGAAAATCCTTTGCT GAATGTTCCAGTCTCATTAAACACAGGAGAATTCACACTGGAGAAAGGCCTTATGAATGTACCAAATGT GGAAAAACATTTCAGCGAAGCTCTACCCTCCTTCATCATCAGAGTTCACACAGGAGAAAGGCCTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297734
Insert Size	1725 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297734.1
RefSeq Size	4706 bp
RefSeq ORF	1725 bp
Locus ID	147687
Protein Families	Transcription Factors
MW	65.6 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter by one amino acid, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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