NUMBL (NM_001289979) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAG3A; CTG3a; NBL; NUMB-R; NUMBLIKE; NUMBR; TNRC23
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336820 representing NM_001289979. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACAAGTTACGGCAGAGCCTGCGGCGGAGGAAGCCAGCCTACGTGCCCGAGGCGTCGCGCCCGCAC CAGTGGCAGGCAGACGAGGACGCGGTGCGGAAGGGCACGTGCAGCTTCCCGGTCAGGTACCTGGGTCAC GTGGAGGTAGAGGAGTCCCGGGGAATGCACGTGTGTGAAGATGCGGTGAAGAAGCTGAAGGCGATGGGC CGAAAGTCCGTGAAGTCTGTCCTGTGGGTGTCAGCCGATGGGCTCCGAGTGGTGGACGACAAAACCAAG GATCTTCTGGTCGACCAGACCATCGAAAAGGTCTCCTTTTGTGCTCCTGACCGCAACCTGGACAAGGCT TTCTCCTATATCTGTCGTGACGGGACTACCCGCCGCTGGATCTGCCACTGTTTTCTGGCACTGAAGGAC TCCGGCGAGAGGCTGAGCCACGCTGTGGGCTGTGCTTTTGCCGCCTGCCTGGAGCGAAAACAGCGACGG GAGAAGGAATGTGGGGTCACGGCCGCCTTCGATGCCAGCCGCACCAGCTTCGCCCGCGAGGGCTCCTTC CGCCTGTCTGGGGGTGGGCGGCCTGCTGAGCGAGAGGCCCCGGACAAGAAGAAAGCAGAGGCAGCAGCT GCCCCCACTGTGGCTCCTGGCCCTGCCCAGCCTGGGCACGTGTCCCCGACACCAGCCACCACATCCCCT GGTGAGAAGGGTGAGGCAGGCACCCCTGTGGCTGCAGGCACCACTGCGGCCGCCATCCCCCGGCGCCAT GCACCCCTGGAGCAGCTGGTTCGCCAGGGCTCCTTCCGTGGGTTCCCAGCACTCAGCCAGAAGAACTCG CCTTTCAAACGGCAGCTGAGCCTACGGCTGAATGAGCTGCCATCCACGCTGCAGCGCCGCACTGACTTC CAGGTGAAGGGCACAGTGCCTGAGATGGAGCCTCCTGGTGCCGGCGACAGTGACAGCATCAACGCTCTG TGCACACAGATCAGTTCATCTTTTGCCAGTGCTGGAGCGCCAGCACCAGGGCCACCACCTGCCACAACA GGGACTTCTGCCTGGGGTGAGCCCTCCGTGCCCCCTGCAGCTGCCTTCCAGCCTGGGCACAAGCGGACA CCTTCAGAGGCTGAGCGATGGCTGGAGGAGGTGTCACAGGTGGCCAAGGCCCAGCAGCAGCAGCAGCAG CAACAGCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAAGCAGCCTCAGTGGCCCCAGTGCCCACC ATGCCTCCTGCCCTGCAGCCTTTCCCCGCCCCCGTGGGGCCCTTTGACGCTGCACCTGCCCAAGTGGCC GTGTTCCTGCCACCCCCACACATGCAGCCCCCTTTTGTGCCCGCCTACCCGGGCTTGGGCTACCCACCG ATGCCCCGGGTGCCCGTGGTGGGCATCACACCCTCACAGATGGTGGCAAACGCCTTCTGCTCAGCCGCC CAGCTCCAGCCTCAGCCTGCCACTCTGCTTGGGAAAGCTGGGGCCTTCCCGCCCCCTGCCATACCCAGT GCCCCTGGGAGCCAGGCCCGCCCTCGCCCCAATGGGGCCCCCTGGCCCCCTGAGCCAGCGCCTGCCCCA GCTCCAGAGTTGGACCCCTTTGAGGCCCAGTGGGCGGCATTAGAAGGCAAAGCCACTGTAGAGAAACCC TCCAACCCCTTTTCTGGCGACCTGCAAAAGACATTCGAGATTGAACTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289979
Insert Size	1707 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289979.1
RefSeq Size	3483 bp
RefSeq ORF	1707 bp
Locus ID	9253
UniProt ID	Q9Y6R0
Protein Pathways	Notch signaling pathway
MW	60.8 kDa
Gene Summary	Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation from a downstream start codon, compared to variant 1. The encoded protein (isoform b) has a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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