NUMBL (NM_001289979) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CAG3A; CTG3a; NBL; NUMB-R; NUMBLIKE; NUMBR; TNRC23
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238926 representing NM_001289979 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAGTTACGGCAGAGCCTGCGGCGGAGGAAGCCAGCCTACGTGCCCGAGGCGTCGCGCCCGCACC AGTGGCAGGCAGACGAGGACGCGGTGCGGAAGGGCACGTGCAGCTTCCCGGTCAGGTACCTGGGTCACGT GGAGGTAGAGGAGTCCCGGGGAATGCACGTGTGTGAAGATGCGGTGAAGAAGCTGAAGGCGATGGGCCGA AAGTCCGTGAAGTCTGTCCTGTGGGTGTCAGCCGATGGGCTCCGAGTGGTGGACGACAAAACCAAGGATC TTCTGGTCGACCAGACCATCGAAAAGGTCTCCTTTTGTGCTCCTGACCGCAACCTGGACAAGGCTTTCTC CTATATCTGTCGTGACGGGACTACCCGCCGCTGGATCTGCCACTGTTTTCTGGCACTGAAGGACTCCGGC GAGAGGCTGAGCCACGCTGTGGGCTGTGCTTTTGCCGCCTGCCTGGAGCGAAAACAGCGACGGGAGAAGG AATGTGGGGTCACGGCCGCCTTCGATGCCAGCCGCACCAGCTTCGCCCGCGAGGGCTCCTTCCGCCTGTC TGGGGGTGGGCGGCCTGCTGAGCGAGAGGCCCCGGACAAGAAGAAAGCAGAGGCAGCAGCTGCCCCCACT GTGGCTCCTGGCCCTGCCCAGCCTGGGCACGTGTCCCCGACACCAGCCACCACATCCCCTGGTGAGAAGG GTGAGGCAGGCACCCCTGTGGCTGCAGGCACCACTGCGGCCGCCATCCCCCGGCGCCATGCACCCCTGGA GCAGCTGGTTCGCCAGGGCTCCTTCCGTGGGTTCCCAGCACTCAGCCAGAAGAACTCGCCTTTCAAACGG CAGCTGAGCCTACGGCTGAATGAGCTGCCATCCACGCTGCAGCGCCGCACTGACTTCCAGGTGAAGGGCA CAGTGCCTGAGATGGAGCCTCCTGGTGCCGGCGACAGTGACAGCATCAACGCTCTGTGCACACAGATCAG TTCATCTTTTGCCAGTGCTGGAGCGCCAGCACCAGGGCCACCACCTGCCACAACAGGGACTTCTGCCTGG GGTGAGCCCTCCGTGCCCCCTGCAGCTGCCTTCCAGCCTGGGCACAAGCGGACACCTTCAGAGGCTGAGC GATGGCTGGAGGAGGTGTCACAGGTGGCCAAGGCCCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCA GCAGCAGCAGCAGCAACAGCAGCAAGCAGCCTCAGTGGCCCCAGTGCCCACCATGCCTCCTGCCCTGCAG CCTTTCCCCGCCCCCGTGGGGCCCTTTGACGCTGCACCTGCCCAAGTGGCCGTGTTCCTGCCACCCCCAC ACATGCAGCCCCCTTTTGTGCCCGCCTACCCGGGCTTGGGCTACCCACCGATGCCCCGGGTGCCCGTGGT GGGCATCACACCCTCACAGATGGTGGCAAACGCCTTCTGCTCAGCCGCCCAGCTCCAGCCTCAGCCTGCC ACTCTGCTTGGGAAAGCTGGGGCCTTCCCGCCCCCTGCCATACCCAGTGCCCCTGGGAGCCAGGCCCGCC CTCGCCCCAATGGGGCCCCCTGGCCCCCTGAGCCAGCGCCTGCCCCAGCTCCAGAGTTGGACCCCTTTGA GGCCCAGTGGGCGGCATTAGAAGGCAAAGCCACTGTAGAGAAACCCTCCAACCCCTTTTCTGGCGACCTG CAAAAGACATTCGAGATTGAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238926 representing NM_001289979 Red=Cloning site Green=Tags(s) MNKLRQSLRRRKPAYVPEASRPHQWQADEDAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGR KSVKSVLWVSADGLRVVDDKTKDLLVDQTIEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSG ERLSHAVGCAFAACLERKQRREKECGVTAAFDASRTSFAREGSFRLSGGGRPAEREAPDKKKAEAAAAPT VAPGPAQPGHVSPTPATTSPGEKGEAGTPVAAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKR QLSLRLNELPSTLQRRTDFQVKGTVPEMEPPGAGDSDSINALCTQISSSFASAGAPAPGPPPATTGTSAW GEPSVPPAAAFQPGHKRTPSEAERWLEEVSQVAKAQQQQQQQQQQQQQQQQQQQQAASVAPVPTMPPALQ PFPAPVGPFDAAPAQVAVFLPPPHMQPPFVPAYPGLGYPPMPRVPVVGITPSQMVANAFCSAAQLQPQPA TLLGKAGAFPPPAIPSAPGSQARPRPNGAPWPPEPAPAPAPELDPFEAQWAALEGKATVEKPSNPFSGDL QKTFEIEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289979
ORF Size	1704 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289979.2
RefSeq Size	3483 bp
RefSeq ORF	1707 bp
Locus ID	9253
UniProt ID	Q9Y6R0
Protein Pathways	Notch signaling pathway
MW	61.3 kDa
Gene Summary	Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.[UniProtKB/Swiss-Prot Function]

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