BIN2 (NM_001290008) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRAP-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336810 representing NM_001290008. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGAGGCATGCCGGGAGCCCGCACTTCCTCCTCGGGGGCCTCAGAAAACCACAGGGCGCGGGGCCAG GGCGGCGGCCCCCAGGGAGTTGGCAGGATGGCAGAGGGCAAGGCAGGCGGCGCGGCCGGCCTCTTCGCC AAGCAGGTGCAGAAGAAGTTTAGCAGGGCCCAGGAGAAGGTGCTGCAGAAATTGGGGAAAGCTGTAGAA ACCAAAGATGAACGATTTGAACAAAGCGCTAGCAACTTCTACCAACAACAGGCAGAAGGCCACAAGCTG TACAAGGACCTGAAGAACTTCCTTAGTGCAGTCAAAGTGATGCATGAAAGTTCAAAAAGAGTGTCAGAA ACCCTGCAGGAGATCTACAGCAGCGAGTGGGACGGTCATGAGGAGCTGAAGGCCATCGTATGGGAGAGA ATTGCCAAGCGGGGTCGGAAACTCGTGGACTATGACAGTGCCCGACACCACCTGGAGGCAGTGCAGAAT GCCAAGAAGAAAGATGAGGCCAAGACTGCCAAGGCAGAGGAAGAGTTCAACAAAGCCCAGACTGTGTTT GAAGATCTGAACCAAGAACTACTAGAGGAGCTGCCTATTCTTTATAATAGTCGTATTGGCTGCTATGTG ACCATCTTCCAAAACATTTCCAACTTGAGGGATGTCTTCTACAGGGAAATGAGCAAGCTGAACCACAAT CTCTACGAGGTGATGAGCAAACTGGAGAAGCAACATTCCAATAAAGTCTTTGTGGTGAAGGGACTGTCA AGCAGCAGCAGGCGCTCTTTAGTCATTTCTCCCCCAGTTCGAACAGCTACAGTCTCCAGTCCTCTTACC TCACCTACTAGTCCCTCTACACTTTCCTTGAAGAGTGAGAGTGAATCTGTCTCAGCAACTGAAGATCTG GCACCTGATGCAGCCCAAGGGGAAGACAATTCTGAGATCAAGGAGCTCTTAGAAGAGGAGGAAATAGAG AAGGAAGGATCTGAAGCAAGCTCCTCTGAGGAAGATGAGCCTCTACCAGCCTGCAATGGCCCCGCCCAG GCCCAGCCCTCTCCTACCACTGAAAGGGCCAAGTCCCAGGAGGAAGTTCTCCCCAGCTCCACAACTCCA TCACCAGGCGGAGCCCTGAGCCCTTCAGGGCAGCCTTCATCATCTGCCACAGAAGTAGTCCTCCGAACC CGCACCGCAAGTGAAGGATCTGAACAACCAAAGAAGAGAGCCTCTATCCAGAGGACCTCAGCACCCCCT AGTAGGCCTCCTCCACCCAGAGCCACTGCAAGCCCCAGGCCCTCCTCAGGGAACATACCTTCCAGCCCT ACAGCCTCTGGAGGGGGTTCACCCACCAGCCCTAGGGCCTCCTTGGGGACTGGGACTGCAAGTCCTAGG ACCTCCCTAGAGGTCTCTCCTAATCCAGAACCACCAGAGAAGCCAGTAAGAACTCCTGAGGCCAAAGAA AATGAAAACATCCACAATCAGAACCCTGAAGAACTTTGTACTTCCCCCACCTTAATGACATCTCAGGTT GCTTCAGAGCCTGGAGAGGCAAAGAAGATGGAAGACAAGGAAAAGGATAATAAGCTTATCTCAGCTAAC TCCTCGGAGGGCCAAGACCAGCTTCAAGTCTCCATGGTACCAGAAAACAACAACCTCACAGCACCTGAA CCTCAAGAAGAGGTATCCACAAGTGAAAATCCACAACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290008
Insert Size	1698 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290008.1
RefSeq Size	2168 bp
RefSeq ORF	1698 bp
Locus ID	51411
UniProt ID	Q9UBW5
MW	61.2 kDa
Gene Summary	Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded protein (isoform 3) is shorter, compared to isoform 1.

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