CDK5RAP1 (NM_001278169) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C20orf34; C42; CGI-05; HSPC167
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336791 representing NM_001278169. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCCGGCCTGAAGTAGCGGCGGAACGGAAGTCGCTTGTGTATGAACGCAGCGGCGGACCTGTGAGG GGATCCGACTTGCCGGCAGAACTTACGCTGCGGGACCCCGGGCACTGTTGCTGCTGCGGGAGTGCCTCA GCTCCTCAGGAGAAGCTGTCTTCAGAAGTGGAAGACCCACCTCCCTATCTCATGATGGATGAACTTCTT GGAAGGCAGAGAAAAGTCTACCTCGAGACCTATGGCTGCCAGATGAATGTGAATGACACAGAGATAGCC TGGTCCATCTTACAGAAGAGTGGCTACCTGCGGACCAGTAACCTCCAAGAGGCAGATGTGATTCTCCTT GTCACATGCTCTATCAGGGAGAAGGCTGAGCAGACCATCTGGAACCGTTTACATCAGCTTAAAGCCTTG AAGACAAGGCGGCCCCGCTCCCGGGTTCCTCTGAGGATTGGAATTCTAGGCTGCATGGCTGAGAGGTTG AAGGAGGAGATTCTCAACAGAGAGAAAATGGTAGATATTTTGGCTGGTCCTGATGCCTACCGGGACCTT CCCCGGCTGCTGGCTGTTGCTGAGTCGGGCCAGCAAGCTGCCAACGTGCTGCTCTCTCTGGACGAGACC TATGCTGATGTCATGCCAGTCCAGACAAGCGCCAGTGCCACGTCTGCCTTTGTGTCAATCATGCGAGGC TGTGACAACATGTGTAGCTACTGCATTGTTCCTTTCACCCGGGGCAGGGAGAGGAGTCGGCCTATTGCC TCCATTCTAGAGGAAGTGAAGAAGCTTTCTGAGCAGGGGCTGAAAGAAGTGACACTTCTTGGTCAGAAT GTTAATAGTTTTCGGGACAATTCGGAGGTCCAGTTCAACAGTGCAGTGCCTACCAATCTCAGTCGTGGC TTTACCACCAACTATAAAACCAAGCAAGGAGGACTTCGTTTTGCTCATCTTCTGGATCAGGTCTCCAGA GTAGATCCTGAAATGAGGATCCGTTTTACCTCTCCCCACCCCAAGGATTTTCCTGATGAGGTTCTGCAG CTGATTCATGAGAGAGATAACATCTGTAAACAGATCCACCTGCCAGCCCAGAGTGGAAGCAGCCGTGTG TTGGAGGCCATGCGGAGGGGATATTCAAGAGAAGCTTATGTGGAGTTAGTTCACCATATTAGAGAATCT ATTCCAGGTGTGAGCCTCAGCAGCGATTTCATTGCTGGCTTTTGTGGTGAGACGGAGGAAGATCACGTC CAGACAGTCTCTTTGCTCCGGGAAGTTCAGTACAACATGGGCTTCCTCTTTGCCTACAGCATGAGACAG AAGACACGGGCATATCATAGGCTGAAGGATGATGTCCCGGAAGAGGTAAAATTAAGGCGTTTGGAGGAA CTCATCACTATCTTCCGAGAAGAAGCAACAAAAGCCAATCAGACCTCTGTGGGCTGTACCCAGTTGGTG CTAGTGGAAGGGCTCAGTAAACGCTCTGCCACTGACCTGTGTGGCAGGAATGATGGAAACCTTAAGGTG ATCTTCCCTGATGCAGAGATGGAGGATGTCAATAACCCTGGGCTCAGGGTCAGAGCCCAGCCTGGGGAC TATGTGCTGGTGAAGATCACCTCAGCCAGTTCTCAGACACTTAGGGGACATGTTCTCTGCAGGACCACT CTGAGGGACTCTTCTGCATATTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278169
Insert Size	1683 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278169.1
RefSeq Size	1911 bp
RefSeq ORF	1683 bp
Locus ID	51654
UniProt ID	Q96SZ6
MW	62.9 kDa
Gene Summary	This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013] Transcript Variant: This variant (5) differs in the 5' UTR and has multiple coding region differences compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (e) with a distinct and shorter N-terminus compared to isoform a.

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