EBF1 (NM_182708) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	COE1; EBF; O/E-1; OLF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336790 representing NM_182708. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCAGCATGAAGGAAGAGCCGCTGGGCAGCGGC ATGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACGGCGGCGCAGAGCGGG GTGGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCCAATCTGCGGAAATCCAACTTCTTCCAC TTCGTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATCGAGAGGACAGCGTTTGTGGGGTTC GTGGAGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAACGGAATTCACTACCGGCTTCAGCTTCTC TACAGCAATGGGATAAGGACGGAGCAGGATTTCTACGTGCGCCTCATTGACTCCATGACAAAACAAGCC ATAGTGTATGAAGGCCAAGACAAGAACCCAGAAATGTGCCGAGTCTTGCTCACACATGAGATCATGTGC AGGTTCTTCTTGAAATTTTTCCTCAAATGTAACCAAAATTGCCTAAAGAATGCGGGAAACCCACGTGAC ATGCGGAGATTCCAGGTCGTGGTGTCTACGACAGTCAATGTGGATGGCCATGTCCTGGCAGTCTCTGAT AACATGTTTGTCCATAATAATTCCAAGCATGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGCTACT CCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGTGCGACTGTGATCATCATAGGG GACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGTTGATCACTCCT CATGCCATCCGTGTGCAGACCCCTCCTCGGCACATCCCTGGTGTTGTGGAAGTCACACTGTCCTACAAA TCTAAGCAGTTCTGCAAAGGAACACCAGGCAGATTCATTTATACAGCGCTCAACGAACCCACCATCGAT TATGGTTTCCAGAGGTTACAGAAGGTCATTCCTCGGCACCCTGGTGACCCTGAGCGTTTGCCAAAGGAA GTAATACTCAAAAGGGCTGCGGATCTGGTAGAAGCACTGTATGGGATGCCACACAACAACCAGGAAATC ATTCTGAAGAGAGCGGCCGACATTGCCGAGGCCCTGTACAGTGTTCCCCGCAACCACAACCAACTCCCG GCCCTTGCTAACACCTCGGTCCACGCAGGGATGATGGGCGTGAATTCGTTCAGTGGACAACTGGCCGTG AATGTCTCCGAGGCATCACAAGCCACCAATCAGGGTTTCACCCGCAACTCAAGCAGCGTATCACCACAC GGGTACGTGCCGAGCACCACTCCCCAGCAGACCAACTATAACTCCGTCACCACGAGCATGAACGGATAC GGCTCTGCCGCAATGTCCAATTTGGGCGGCTCCCCCACCTTCCTCAACGGCTCAGCTGCCAACTCCCCC TATGCCATAGTGCCATCCAGCCCCACCATGGCCTCCTCCACAAGCCTCCCCTCCAACTGCAGCAGCTCC TCGGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCCGTGAAACAGAAGAGTGCTTTCGCACCA GTCGTCAGACCCCAGACCTCCCCACCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATA TCTGGCATGATTGTTCCTCCTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_182708
Insert Size	1683 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_182708.2
RefSeq Size	5171 bp
RefSeq ORF	1683 bp
Locus ID	1879
UniProt ID	Q9UH73
Protein Families	Transcription Factors
MW	61.1 kDa
Gene Summary	Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon and uses alternate in-frame splice junctions at the ends of two exons compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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